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The map label for this gene is acoL [H]

Identifier: 52784650

GI number: 52784650

Start: 870767

End: 872143

Strand: Direct

Name: acoL [H]

Synonym: BLi00852

Alternate gene names: 52784650

Gene position: 870767-872143 (Clockwise)

Preceding gene: 52784649

Following gene: 52784651

Centisome position: 20.62

GC content: 51.56

Gene sequence:

>1377_bases
GTGACACTTGCAATTATAGGAGGGGGACCTGCGGGTTACGTGGCGGCGATTACAGCGGCCAAAAATAGCAGAGAAGTCAT
CCTGATTGAAGATGGTTCCCTCGGAGGAACGTGTTTAAATGAAGGATGCATACCGACCAAAGCGCTTCTTGAAAGCGCAG
ATATGTTCGAGAAAATAAAATCCGCCGGCGCATTCGGAATCGGGCTGTCAGGCGAAAGCCCGACGGTTCAGTGGGAGGCC
GTCCAAAGCCGTAAGAGAGAGATCATTCAGCGGCTCGCCGGGGGTATTGAATATTTGATGAATAAAAATAAAATCAAGGT
TCTGCAAGGAAAAGCATCGTTTTTGTCCGAACACGACATACTTGTTGAAAAGGACGGAAAACAGGAGGTCATCAATGCCG
GGCATGTGATTATAGCGGCAGGGGCAGCACCTGCCGCACTGCCTTTTGCGTCTTTTGACGGGAGCTGGATCATCCACAGC
GGAGATGCCATGTCATTGCCGTCCATCCCTGAAACGATTTTTATTATCGGCGGAGGAATCATCGGCTGTGAATTTGCCAG
TATATACAGCAGGCTGGGAGCGAACGTGATCATAGCCGAGCAGGCGGAACAGATTCTGCCTGAGGAAGATCCGGATATTG
CCGCCTTTCTTCGCACAAGTCTCGAGGAGTCCGGCGTCCGTGTTTTCACATCTGTACAAGTGGAGCGTCTTGATCCTGCC
AGGCAGCGCGTCTTTCTGAAAAATGGCGATGACAGCTTTAATATTCAAGCTGATCGATGTCTCGTCGCCGTCGGCCGAAA
GCCGAGGACTGATGATCTGAAGCTTGACCATATCGGAGTCCAATATGACAGGAAAGGGATTCATGTCAACCAGCATATGC
AGACAAACATCCCGCATATTTATGCGTGCGGGGATATCACAGGCGGGGTTCAGCTGGCGCATGCCGCTTTTCACGAAGGA
ACCGTCGCCGCATCCCATGCCTCAGGCAGAGACATGAAGGTCAACAGTGACATTATTCCGCGCTGCATCTATACTTCGCC
TGAAATCGCAAGCGTCGGCTTAAATGAAAAAAAGGCGAGGGAGCGCTACAGGGATGTTCGTATCGGAGAGTTTCCGTTTT
CTTCAAACGGAAAAGCGCTTATTTTGAATGAAACTGCCGGCAAGGTCAAAATCATCATAGAGCCGGAGTATCAGGAAATT
GTCGGGGTTTCGATCGTGGGTCCGGGAGCAACCGAACTGATCGGCCAGGCAGCCGTAATGTTGCATGCAGAGCTGACGGC
AGACGCAATGGAGCACTTCATCGCGGCCCATCCGACTCTTTCGGAAACGATTCACGAAGCTTTGCTGCAAGCCGCAGGTC
GGGCCATTCACTGCTGA

Upstream 100 bases:

>100_bases
CGCGCTTGATGGTGCGCCTGCTGCAGCATTCCTTTCAGATGTGAAAAACTATTTGGAAGATCCGGCATCAATTCTTTTAT
AGAGAGAAGGTGGATAGACT

Downstream 100 bases:

>100_bases
ATAAGGGAGAACTTCGCACAGCGTGAGAGTGCGGAGTTTTTTTTATCGATATTGAAAGCGCTTTTATAATATAATGAGGA
AAAAGCATGAGGAGAGGATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTLAIIGGGPAGYVAAITAAKNSREVILIEDGSLGGTCLNEGCIPTKALLESADMFEKIKSAGAFGIGLSGESPTVQWEA
VQSRKREIIQRLAGGIEYLMNKNKIKVLQGKASFLSEHDILVEKDGKQEVINAGHVIIAAGAAPAALPFASFDGSWIIHS
GDAMSLPSIPETIFIIGGGIIGCEFASIYSRLGANVIIAEQAEQILPEEDPDIAAFLRTSLEESGVRVFTSVQVERLDPA
RQRVFLKNGDDSFNIQADRCLVAVGRKPRTDDLKLDHIGVQYDRKGIHVNQHMQTNIPHIYACGDITGGVQLAHAAFHEG
TVAASHASGRDMKVNSDIIPRCIYTSPEIASVGLNEKKARERYRDVRIGEFPFSSNGKALILNETAGKVKIIIEPEYQEI
VGVSIVGPGATELIGQAAVMLHAELTADAMEHFIAAHPTLSETIHEALLQAAGRAIHC

Sequences:

>Translated_458_residues
MTLAIIGGGPAGYVAAITAAKNSREVILIEDGSLGGTCLNEGCIPTKALLESADMFEKIKSAGAFGIGLSGESPTVQWEA
VQSRKREIIQRLAGGIEYLMNKNKIKVLQGKASFLSEHDILVEKDGKQEVINAGHVIIAAGAAPAALPFASFDGSWIIHS
GDAMSLPSIPETIFIIGGGIIGCEFASIYSRLGANVIIAEQAEQILPEEDPDIAAFLRTSLEESGVRVFTSVQVERLDPA
RQRVFLKNGDDSFNIQADRCLVAVGRKPRTDDLKLDHIGVQYDRKGIHVNQHMQTNIPHIYACGDITGGVQLAHAAFHEG
TVAASHASGRDMKVNSDIIPRCIYTSPEIASVGLNEKKARERYRDVRIGEFPFSSNGKALILNETAGKVKIIIEPEYQEI
VGVSIVGPGATELIGQAAVMLHAELTADAMEHFIAAHPTLSETIHEALLQAAGRAIHC
>Mature_457_residues
TLAIIGGGPAGYVAAITAAKNSREVILIEDGSLGGTCLNEGCIPTKALLESADMFEKIKSAGAFGIGLSGESPTVQWEAV
QSRKREIIQRLAGGIEYLMNKNKIKVLQGKASFLSEHDILVEKDGKQEVINAGHVIIAAGAAPAALPFASFDGSWIIHSG
DAMSLPSIPETIFIIGGGIIGCEFASIYSRLGANVIIAEQAEQILPEEDPDIAAFLRTSLEESGVRVFTSVQVERLDPAR
QRVFLKNGDDSFNIQADRCLVAVGRKPRTDDLKLDHIGVQYDRKGIHVNQHMQTNIPHIYACGDITGGVQLAHAAFHEGT
VAASHASGRDMKVNSDIIPRCIYTSPEIASVGLNEKKARERYRDVRIGEFPFSSNGKALILNETAGKVKIIIEPEYQEIV
GVSIVGPGATELIGQAAVMLHAELTADAMEHFIAAHPTLSETIHEALLQAAGRAIHC

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=33.1182795698925, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI50301238, Length=438, Percent_Identity=27.8538812785388, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=26.5734265734266, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=26.5734265734266, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=26.5734265734266, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=26.5734265734266, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI148277071, Length=429, Percent_Identity=26.5734265734266, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI291045266, Length=429, Percent_Identity=26.5734265734266, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=25.7009345794392, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=25.2955082742317, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1786307, Length=447, Percent_Identity=34.0044742729307, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=29.1759465478842, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=29.0672451193059, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=29.0617848970252, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=32.8326180257511, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17557007, Length=466, Percent_Identity=27.2532188841202, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=433, Percent_Identity=24.7113163972286, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=433, Percent_Identity=24.7113163972286, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=440, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=36.6525423728814, Blast_Score=259, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6325240, Length=467, Percent_Identity=29.3361884368308, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=25.9911894273128, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21358499, Length=465, Percent_Identity=34.1935483870968, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24640551, Length=464, Percent_Identity=26.9396551724138, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640549, Length=464, Percent_Identity=26.9396551724138, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640553, Length=464, Percent_Identity=26.9396551724138, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17737741, Length=468, Percent_Identity=25.4273504273504, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49026; Mature: 48894

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAIIGGGPAGYVAAITAAKNSREVILIEDGSLGGTCLNEGCIPTKALLESADMFEKIK
CEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SAGAFGIGLSGESPTVQWEAVQSRKREIIQRLAGGIEYLMNKNKIKVLQGKASFLSEHDI
HCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCE
LVEKDGKQEVINAGHVIIAAGAAPAALPFASFDGSWIIHSGDAMSLPSIPETIFIIGGGI
EEECCCCHHHHCCCCEEEECCCCCCCCEEECCCCCEEEECCCCCCCCCCCCEEEEEECCE
IGCEFASIYSRLGANVIIAEQAEQILPEEDPDIAAFLRTSLEESGVRVFTSVQVERLDPA
EHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCHH
RQRVFLKNGDDSFNIQADRCLVAVGRKPRTDDLKLDHIGVQYDRKGIHVNQHMQTNIPHI
HHEEEEECCCCCEEEECCEEEEEECCCCCCCCEEEEEECEEECCCCCEECCCHHCCCCEE
YACGDITGGVQLAHAAFHEGTVAASHASGRDMKVNSDIIPRCIYTSPEIASVGLNEKKAR
EEECCCCCCHHHHHHHHCCCCEEECCCCCCCEEECCCCCCEEEECCCCCEECCCCHHHHH
ERYRDVRIGEFPFSSNGKALILNETAGKVKIIIEPEYQEIVGVSIVGPGATELIGQAAVM
HHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECCCHHHHHHHHHEE
LHAELTADAMEHFIAAHPTLSETIHEALLQAAGRAIHC
EEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TLAIIGGGPAGYVAAITAAKNSREVILIEDGSLGGTCLNEGCIPTKALLESADMFEKIK
EEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SAGAFGIGLSGESPTVQWEAVQSRKREIIQRLAGGIEYLMNKNKIKVLQGKASFLSEHDI
HCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCE
LVEKDGKQEVINAGHVIIAAGAAPAALPFASFDGSWIIHSGDAMSLPSIPETIFIIGGGI
EEECCCCHHHHCCCCEEEECCCCCCCCEEECCCCCEEEECCCCCCCCCCCCEEEEEECCE
IGCEFASIYSRLGANVIIAEQAEQILPEEDPDIAAFLRTSLEESGVRVFTSVQVERLDPA
EHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCHH
RQRVFLKNGDDSFNIQADRCLVAVGRKPRTDDLKLDHIGVQYDRKGIHVNQHMQTNIPHI
HHEEEEECCCCCEEEECCEEEEEECCCCCCCCEEEEEECEEECCCCCEECCCHHCCCCEE
YACGDITGGVQLAHAAFHEGTVAASHASGRDMKVNSDIIPRCIYTSPEIASVGLNEKKAR
EEECCCCCCHHHHHHHHCCCCEEECCCCCCCEEECCCCCCEEEECCCCCEECCCCHHHHH
ERYRDVRIGEFPFSSNGKALILNETAGKVKIIIEPEYQEIVGVSIVGPGATELIGQAAVM
HHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECCCHHHHHHHHHEE
LHAELTADAMEHFIAAHPTLSETIHEALLQAAGRAIHC
EEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]