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The map label for this gene is acoC [H]

Identifier: 52784649

GI number: 52784649

Start: 869615

End: 870748

Strand: Direct

Name: acoC [H]

Synonym: BLi00851

Alternate gene names: 52784649

Gene position: 869615-870748 (Clockwise)

Preceding gene: 52784648

Following gene: 52784650

Centisome position: 20.59

GC content: 51.59

Gene sequence:

>1134_bases
ATGGCTGTCGAAGTGGTCATGCCGAAATTGGGGATGTCGATGAAAGAAGGTACGGTTTCCGTCTGGAATAAAAAAGTCGG
GGAAGCCGTTGAAAAAGGAGAAAGCATTGCCAGCATCAATTCCGAAAAAATCGAAATGGAAATCGAATCTCCGGCAAACG
GAACCGTGTTGGACATCCAAGTATCTGAAGGAGAGGGAGTGCCGCCGGGAACCGTCATCTGCCGCATCGGAAATGAAAAC
GAACAAACGCAGGAGAGCCAAACAAAACAGCCGGATCCCACGAAAGAACGGATTAAAATTTCTCCGGCTGCGCGCAAAAT
TGCGCAAAGCGCAAATATAGACATCAAAACGCTGAAGGGCACAGGCCCCGGGGGGAGAATTACGAAAGCGGATGTGTTGC
AGGCGCTTCCCGGCCGGCCGAACAAGCAGGCGGCTAAGGCTGAAGCTGAAGAGCGGCCGCCGGCAAGCCCGATGAGGAAA
ACGATAGCCGCAAGAATGACGGAAAGCCTGCAAAACAGCGCCCAGCTGACGATTACGATGAAGGCGGACATCACGAAATT
AACAGTCCTGCAAAAACAGCTTAATGAAACCGCTCAATCAAGATACGATACGAAGCTGACGATCACTGATTTTGCCGCCA
GGGCAGCTGTTTTTTCGCTTTTGGATCATCCGGCCATGAACAGTGTTTATCAAGACGGAAGGCTTGCGACCTTTGAGCAT
GTTCATTTAGGAGTGGCGGCCGCTTTGGACGAAGGCCTGGCCGTTCCCGTCATCCGCCATGCCGAGCGGCTCCCGCTGAT
CGAATTGGCCAAGAAAATCAAATGGTACGCCAAAAAAGCGCGGGAAGGCCGGCTTCTACACGATGAAATAGAAGGATCGA
CATTTACGATTACAAACCTCGGCGCATATGGTGTCGAGCACTTTACCCCGATCTTGAATCCGCCGGAAACCGGGATACTC
GGAGTCGGCCAAATGTATTCGGCACCCGTTTATCAAGACGGAGAACTGACAAAGGGCGCCATTCTGCCGCTCAGTCTGAC
TTTTGATCACCGCGCGCTTGATGGTGCGCCTGCTGCAGCATTCCTTTCAGATGTGAAAAACTATTTGGAAGATCCGGCAT
CAATTCTTTTATAG

Upstream 100 bases:

>100_bases
CTGTATTTGCCGACGCCGGAAAAAGTCGTGAACACCGTGCTTGAGATGCTCGGAGAAACATCAACACATACGGTGTAAAG
ATTGGAAAGGAGAGGAGAAC

Downstream 100 bases:

>100_bases
AGAGAAGGTGGATAGACTGTGACACTTGCAATTATAGGAGGGGGACCTGCGGGTTACGTGGCGGCGATTACAGCGGCCAA
AAATAGCAGAGAAGTCATCC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAVEVVMPKLGMSMKEGTVSVWNKKVGEAVEKGESIASINSEKIEMEIESPANGTVLDIQVSEGEGVPPGTVICRIGNEN
EQTQESQTKQPDPTKERIKISPAARKIAQSANIDIKTLKGTGPGGRITKADVLQALPGRPNKQAAKAEAEERPPASPMRK
TIAARMTESLQNSAQLTITMKADITKLTVLQKQLNETAQSRYDTKLTITDFAARAAVFSLLDHPAMNSVYQDGRLATFEH
VHLGVAAALDEGLAVPVIRHAERLPLIELAKKIKWYAKKAREGRLLHDEIEGSTFTITNLGAYGVEHFTPILNPPETGIL
GVGQMYSAPVYQDGELTKGAILPLSLTFDHRALDGAPAAAFLSDVKNYLEDPASILL

Sequences:

>Translated_377_residues
MAVEVVMPKLGMSMKEGTVSVWNKKVGEAVEKGESIASINSEKIEMEIESPANGTVLDIQVSEGEGVPPGTVICRIGNEN
EQTQESQTKQPDPTKERIKISPAARKIAQSANIDIKTLKGTGPGGRITKADVLQALPGRPNKQAAKAEAEERPPASPMRK
TIAARMTESLQNSAQLTITMKADITKLTVLQKQLNETAQSRYDTKLTITDFAARAAVFSLLDHPAMNSVYQDGRLATFEH
VHLGVAAALDEGLAVPVIRHAERLPLIELAKKIKWYAKKAREGRLLHDEIEGSTFTITNLGAYGVEHFTPILNPPETGIL
GVGQMYSAPVYQDGELTKGAILPLSLTFDHRALDGAPAAAFLSDVKNYLEDPASILL
>Mature_376_residues
AVEVVMPKLGMSMKEGTVSVWNKKVGEAVEKGESIASINSEKIEMEIESPANGTVLDIQVSEGEGVPPGTVICRIGNENE
QTQESQTKQPDPTKERIKISPAARKIAQSANIDIKTLKGTGPGGRITKADVLQALPGRPNKQAAKAEAEERPPASPMRKT
IAARMTESLQNSAQLTITMKADITKLTVLQKQLNETAQSRYDTKLTITDFAARAAVFSLLDHPAMNSVYQDGRLATFEHV
HLGVAAALDEGLAVPVIRHAERLPLIELAKKIKWYAKKAREGRLLHDEIEGSTFTITNLGAYGVEHFTPILNPPETGILG
VGQMYSAPVYQDGELTKGAILPLSLTFDHRALDGAPAAAFLSDVKNYLEDPASILL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=29.4663573085847, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=26.5486725663717, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=26.5486725663717, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=32.034632034632, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=26.3157894736842, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI260898739, Length=140, Percent_Identity=35, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786946, Length=401, Percent_Identity=29.925187032419, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1786305, Length=402, Percent_Identity=29.6019900497512, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=31.002331002331, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=394, Percent_Identity=27.9187817258883, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=24.4604316546763, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=31.3636363636364, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=28.428927680798, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=27.9642058165548, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=29.7945205479452, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=29.7945205479452, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=26.4018691588785, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 40734; Mature: 40603

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVVMPKLGMSMKEGTVSVWNKKVGEAVEKGESIASINSEKIEMEIESPANGTVLDIQ
CCEEEECHHHCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCCCEEEEE
VSEGEGVPPGTVICRIGNENEQTQESQTKQPDPTKERIKISPAARKIAQSANIDIKTLKG
ECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHEEECHHHHHHHHHCCCEEEEEEC
TGPGGRITKADVLQALPGRPNKQAAKAEAEERPPASPMRKTIAARMTESLQNSAQLTITM
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
KADITKLTVLQKQLNETAQSRYDTKLTITDFAARAAVFSLLDHPAMNSVYQDGRLATFEH
ECCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHH
VHLGVAAALDEGLAVPVIRHAERLPLIELAKKIKWYAKKAREGRLLHDEIEGSTFTITNL
HHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCEEEEECC
GAYGVEHFTPILNPPETGILGVGQMYSAPVYQDGELTKGAILPLSLTFDHRALDGAPAAA
CHHHHHHCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHH
FLSDVKNYLEDPASILL
HHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
AVEVVMPKLGMSMKEGTVSVWNKKVGEAVEKGESIASINSEKIEMEIESPANGTVLDIQ
CEEEECHHHCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCCCEEEEE
VSEGEGVPPGTVICRIGNENEQTQESQTKQPDPTKERIKISPAARKIAQSANIDIKTLKG
ECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHEEECHHHHHHHHHCCCEEEEEEC
TGPGGRITKADVLQALPGRPNKQAAKAEAEERPPASPMRKTIAARMTESLQNSAQLTITM
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
KADITKLTVLQKQLNETAQSRYDTKLTITDFAARAAVFSLLDHPAMNSVYQDGRLATFEH
ECCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHH
VHLGVAAALDEGLAVPVIRHAERLPLIELAKKIKWYAKKAREGRLLHDEIEGSTFTITNL
HHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCEEEEECC
GAYGVEHFTPILNPPETGILGVGQMYSAPVYQDGELTKGAILPLSLTFDHRALDGAPAAA
CHHHHHHCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHH
FLSDVKNYLEDPASILL
HHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]