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The map label for this gene is rocR [H]

Identifier: 52784241

GI number: 52784241

Start: 419599

End: 421104

Strand: Reverse

Name: rocR [H]

Synonym: BLi00421

Alternate gene names: 52784241

Gene position: 421104-419599 (Counterclockwise)

Preceding gene: 52784245

Following gene: 52784240

Centisome position: 9.97

GC content: 47.28

Gene sequence:

>1506_bases
ATGCAAAGGTGCAAAAATAAATGCATATTTTTTTGCACCCTTATCAAGTCTTCACTTAAAATGGAGACAGATACGAACAA
AGGGGGATATCAATTGCTGAAGGATTATGATTTTCTGAAACTTATTTTTCAAGGAATTATAGATGAAATCGATATCGGCT
TGCATGTCGTCGATGAAAACGGCACATCTGTTGTCTATAACAAAAAGATGAGCCAGATTGAAGGGATGGATGTCGGCGAT
GTGCTCGGCAAAAACGTGCTGGACGTGTTTACGTTTGCCAGCCAGCATGACAGCACCCTCCTGCAGGCGCTGCATCACGG
GAAAACGAACAAAAACGTCAAACAGACTTACTTTAATAACAAAGGACAGGAAATTACGACCGTCAATCATACATTCCCCA
TTATGGAAAACGGGAATACGAAAGGAGCGGTCGAGATTGCCAAAGATGTCACAAAGCTTGAACGCCTGATCAGAGAAAAT
ATGAACAAAACAGAAAGCACAAAGTATACGTTTGACAGCCTGATCGGCGTCAGTCCGGCGTTCAAGGAAGTCATTGAACA
TGCAAAGCGGGCGACCCGGACCTCTTCCTCCATTCTAATCGTTGGCGACACCGGAACCGGAAAAGAACTGTTCGCCCAAA
GTATACATAACGGAAGCCAGCGCTCAACGGGCCCATTCATCTCGCAAAACTGTGCAGCCCTCCCGGAAAGCCTTGTCGAA
GGCCTATTATTCGGCACGGTCAAAGGCGCATTCACGGGAGCCGTGGATCGTCCGGGTCTTTTCGAACAAGCTGACGGCGG
AACACTGCTCCTTGACGAAATCAATTCTTTGGACTTCAGATTGCAGGCAAAGCTTTTGCGCGCGATTCAAGAAAAAACGA
TCAGGCGGATCGGCGCTTCAAAGGATACTCCGATCGACGTCAGGATCATCGCGACGATGAACGAAGATCCAGTCGACGCT
GTCAGCGGCCAGAGGCTGCGAAAAGATTTGTATTACAGGCTGAGCGTCGTCACCTTGTTTATTCCTCCATTAAAAGACCG
GAAGGAAGATATTATGCCGCTCACGCAGCATTTTATCGACAAATACAACGCATTGTTCCAAATGGAGGTCAAGGGTTTTG
AAGAAGAGGTGCGGCGGTTCCTGCTTTCATACGATTGGCCGGGAAACGTCAGGGAGCTTGAACATTTAATTGAAGGCGCC
ATGAATTTGATGTCCTACGAAGACAAAATCGAGCTGACGCACCTTCCATTGCAATACAGGACGAAACCAGCGGCAAAAGA
GCAGCTGCCGCAGCAGGGCTATGATCTGTTCGCCCCGCTTCCTTCCGCCTCGGCCGCTCCTTTAAAGGAACAAATCGAGA
ATGCCGAAAAGTATTATATTCAAAAAACCGTTAAAAAATGCAATTACAATGTGTCACAAGCGGCTCGGGTGCTGGGGATC
AGCAGGCAAAGCCTGCAATACAGGCTTAAAAAATGGAAGATCCGCTTCGATCAGGAATCAGAATGA

Upstream 100 bases:

>100_bases
TTGTTTCACACTATCTTATATGCAAGTATCGTGCCAACTCTTAATACCGCAAATTTTCAGAACATTGCATGTGTAACTCG
CAAAAAATTTTGCGGTTTTT

Downstream 100 bases:

>100_bases
TCTTCTCTCCCTTTCATCACTAAAAACGTATTTCTTGACTCATACTAAAAAAGGTTTATGCTTTACTTTTGTAAGAGCCT
TAAGTAAAATCATATCAACT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MQRCKNKCIFFCTLIKSSLKMETDTNKGGYQLLKDYDFLKLIFQGIIDEIDIGLHVVDENGTSVVYNKKMSQIEGMDVGD
VLGKNVLDVFTFASQHDSTLLQALHHGKTNKNVKQTYFNNKGQEITTVNHTFPIMENGNTKGAVEIAKDVTKLERLIREN
MNKTESTKYTFDSLIGVSPAFKEVIEHAKRATRTSSSILIVGDTGTGKELFAQSIHNGSQRSTGPFISQNCAALPESLVE
GLLFGTVKGAFTGAVDRPGLFEQADGGTLLLDEINSLDFRLQAKLLRAIQEKTIRRIGASKDTPIDVRIIATMNEDPVDA
VSGQRLRKDLYYRLSVVTLFIPPLKDRKEDIMPLTQHFIDKYNALFQMEVKGFEEEVRRFLLSYDWPGNVRELEHLIEGA
MNLMSYEDKIELTHLPLQYRTKPAAKEQLPQQGYDLFAPLPSASAAPLKEQIENAEKYYIQKTVKKCNYNVSQAARVLGI
SRQSLQYRLKKWKIRFDQESE

Sequences:

>Translated_501_residues
MQRCKNKCIFFCTLIKSSLKMETDTNKGGYQLLKDYDFLKLIFQGIIDEIDIGLHVVDENGTSVVYNKKMSQIEGMDVGD
VLGKNVLDVFTFASQHDSTLLQALHHGKTNKNVKQTYFNNKGQEITTVNHTFPIMENGNTKGAVEIAKDVTKLERLIREN
MNKTESTKYTFDSLIGVSPAFKEVIEHAKRATRTSSSILIVGDTGTGKELFAQSIHNGSQRSTGPFISQNCAALPESLVE
GLLFGTVKGAFTGAVDRPGLFEQADGGTLLLDEINSLDFRLQAKLLRAIQEKTIRRIGASKDTPIDVRIIATMNEDPVDA
VSGQRLRKDLYYRLSVVTLFIPPLKDRKEDIMPLTQHFIDKYNALFQMEVKGFEEEVRRFLLSYDWPGNVRELEHLIEGA
MNLMSYEDKIELTHLPLQYRTKPAAKEQLPQQGYDLFAPLPSASAAPLKEQIENAEKYYIQKTVKKCNYNVSQAARVLGI
SRQSLQYRLKKWKIRFDQESE
>Mature_501_residues
MQRCKNKCIFFCTLIKSSLKMETDTNKGGYQLLKDYDFLKLIFQGIIDEIDIGLHVVDENGTSVVYNKKMSQIEGMDVGD
VLGKNVLDVFTFASQHDSTLLQALHHGKTNKNVKQTYFNNKGQEITTVNHTFPIMENGNTKGAVEIAKDVTKLERLIREN
MNKTESTKYTFDSLIGVSPAFKEVIEHAKRATRTSSSILIVGDTGTGKELFAQSIHNGSQRSTGPFISQNCAALPESLVE
GLLFGTVKGAFTGAVDRPGLFEQADGGTLLLDEINSLDFRLQAKLLRAIQEKTIRRIGASKDTPIDVRIIATMNEDPVDA
VSGQRLRKDLYYRLSVVTLFIPPLKDRKEDIMPLTQHFIDKYNALFQMEVKGFEEEVRRFLLSYDWPGNVRELEHLIEGA
MNLMSYEDKIELTHLPLQYRTKPAAKEQLPQQGYDLFAPLPSASAAPLKEQIENAEKYYIQKTVKKCNYNVSQAARVLGI
SRQSLQYRLKKWKIRFDQESE

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=352, Percent_Identity=39.7727272727273, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790437, Length=319, Percent_Identity=41.3793103448276, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI87082117, Length=364, Percent_Identity=38.4615384615385, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788905, Length=313, Percent_Identity=38.9776357827476, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789087, Length=349, Percent_Identity=37.5358166189112, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI87082152, Length=348, Percent_Identity=36.4942528735632, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789233, Length=472, Percent_Identity=31.1440677966102, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786524, Length=375, Percent_Identity=34.4, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790299, Length=327, Percent_Identity=35.7798165137615, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1787583, Length=263, Percent_Identity=39.5437262357414, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=36.3914373088685, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI87081858, Length=461, Percent_Identity=27.765726681128, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1789828, Length=341, Percent_Identity=31.6715542521994, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56696; Mature: 56696

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRCKNKCIFFCTLIKSSLKMETDTNKGGYQLLKDYDFLKLIFQGIIDEIDIGLHVVDEN
CCCHHHHHEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GTSVVYNKKMSQIEGMDVGDVLGKNVLDVFTFASQHDSTLLQALHHGKTNKNVKQTYFNN
CCEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHCCC
KGQEITTVNHTFPIMENGNTKGAVEIAKDVTKLERLIRENMNKTESTKYTFDSLIGVSPA
CCCEEEEECCEECEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHH
FKEVIEHAKRATRTSSSILIVGDTGTGKELFAQSIHNGSQRSTGPFISQNCAALPESLVE
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
GLLFGTVKGAFTGAVDRPGLFEQADGGTLLLDEINSLDFRLQAKLLRAIQEKTIRRIGAS
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
KDTPIDVRIIATMNEDPVDAVSGQRLRKDLYYRLSVVTLFIPPLKDRKEDIMPLTQHFID
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
KYNALFQMEVKGFEEEVRRFLLSYDWPGNVRELEHLIEGAMNLMSYEDKIELTHLPLQYR
HHHHEEEHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHC
TKPAAKEQLPQQGYDLFAPLPSASAAPLKEQIENAEKYYIQKTVKKCNYNVSQAARVLGI
CCCCHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
SRQSLQYRLKKWKIRFDQESE
CHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MQRCKNKCIFFCTLIKSSLKMETDTNKGGYQLLKDYDFLKLIFQGIIDEIDIGLHVVDEN
CCCHHHHHEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GTSVVYNKKMSQIEGMDVGDVLGKNVLDVFTFASQHDSTLLQALHHGKTNKNVKQTYFNN
CCEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHCCC
KGQEITTVNHTFPIMENGNTKGAVEIAKDVTKLERLIRENMNKTESTKYTFDSLIGVSPA
CCCEEEEECCEECEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHH
FKEVIEHAKRATRTSSSILIVGDTGTGKELFAQSIHNGSQRSTGPFISQNCAALPESLVE
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
GLLFGTVKGAFTGAVDRPGLFEQADGGTLLLDEINSLDFRLQAKLLRAIQEKTIRRIGAS
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
KDTPIDVRIIATMNEDPVDAVSGQRLRKDLYYRLSVVTLFIPPLKDRKEDIMPLTQHFID
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
KYNALFQMEVKGFEEEVRRFLLSYDWPGNVRELEHLIEGAMNLMSYEDKIELTHLPLQYR
HHHHEEEHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHC
TKPAAKEQLPQQGYDLFAPLPSASAAPLKEQIENAEKYYIQKTVKKCNYNVSQAARVLGI
CCCCHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
SRQSLQYRLKKWKIRFDQESE
CHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]