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The map label for this gene is yckK [H]

Identifier: 52784240

GI number: 52784240

Start: 418664

End: 419470

Strand: Reverse

Name: yckK [H]

Synonym: BLi00420

Alternate gene names: 52784240

Gene position: 419470-418664 (Counterclockwise)

Preceding gene: 52784241

Following gene: 52784239

Centisome position: 9.93

GC content: 46.34

Gene sequence:

>807_bases
ATGAAGAAAGCAATATTCGCTGTATTATTGACGCTGTTGATCACCGTGATGGCCGCATGCGGCGCGTCAGGGGACAATTC
CAAGGATAAAAACGCAGCCGGCGACAGCCTCTGGTCTTCAATTAAAGATAAAGGCGTATTGACAGTCGGTACTGAAGGAA
CGTACGAACCTTTTACATTCCACGATAAAAAAACAGATAAATTAACAGGTTATGATGTCGAAGTGATGCAGGAAGTCGCC
AAGCGCCTCAACCTGAAAGCTGAATTTAAAGAAACGCAGTGGGACAGCATGTTTGCCGGACTCAACTCAAAGCGTTTTGA
CGTCGTTGCCAACCAAGTCGGGAAAAAAGACCGTGAAGATCAGTACGATTTTTCTGATTCATATACAACTTCCCAAGCCG
TCGTTGTCACAAAAGCGGACAACAACGATGTAAAGTCTGAAGGAGATGTCAAAGGGAAAAAAGCCGCGCAGTCTTTGACA
AGCAACTATAATGACCTTGCGAAAAACGCCGGCGCTGAAATCGAAGGCGTAGAAGGAATGGCTCAAGCGTTTCAGCTGAT
TCAGCAGGGCCGTGTCGACCTCACCTATAACGATAAACTGGCGGTGCTGAACTATTTGAAGACGTCAGGAAACAAAAACC
TGAAAATCGCTTTTGAAACAGGCGAGCCGCAAACTACGCATTTTGCTTTCCGCAAAGGAAGCGGCGAACTGGTTGACAAG
GTCAACTCCGCGCTGAAGGACATGAAAGAGGATGGCACACTCGCGAAAATAGCGAAGAAATGGTTTGGCGAAGATGTTTC
TAAGTAA

Upstream 100 bases:

>100_bases
TATTTCTTGACTCATACTAAAAAAGGTTTATGCTTTACTTTTGTAAGAGCCTTAAGTAAAATCATATCAACTAAATCCAA
TCAGTAAAGTGGGGAATAAA

Downstream 100 bases:

>100_bases
TATACAAGCGGCGGCCATCGGCCAGGCGATCCCGTGGGATCTTGTGCGTGAGTCATTTTGGCCGATCTTTTTAAGGGGAA
TCTATTACTCGATTCCCCTT

Product: YckK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKKAIFAVLLTLLITVMAACGASGDNSKDKNAAGDSLWSSIKDKGVLTVGTEGTYEPFTFHDKKTDKLTGYDVEVMQEVA
KRLNLKAEFKETQWDSMFAGLNSKRFDVVANQVGKKDREDQYDFSDSYTTSQAVVVTKADNNDVKSEGDVKGKKAAQSLT
SNYNDLAKNAGAEIEGVEGMAQAFQLIQQGRVDLTYNDKLAVLNYLKTSGNKNLKIAFETGEPQTTHFAFRKGSGELVDK
VNSALKDMKEDGTLAKIAKKWFGEDVSK

Sequences:

>Translated_268_residues
MKKAIFAVLLTLLITVMAACGASGDNSKDKNAAGDSLWSSIKDKGVLTVGTEGTYEPFTFHDKKTDKLTGYDVEVMQEVA
KRLNLKAEFKETQWDSMFAGLNSKRFDVVANQVGKKDREDQYDFSDSYTTSQAVVVTKADNNDVKSEGDVKGKKAAQSLT
SNYNDLAKNAGAEIEGVEGMAQAFQLIQQGRVDLTYNDKLAVLNYLKTSGNKNLKIAFETGEPQTTHFAFRKGSGELVDK
VNSALKDMKEDGTLAKIAKKWFGEDVSK
>Mature_268_residues
MKKAIFAVLLTLLITVMAACGASGDNSKDKNAAGDSLWSSIKDKGVLTVGTEGTYEPFTFHDKKTDKLTGYDVEVMQEVA
KRLNLKAEFKETQWDSMFAGLNSKRFDVVANQVGKKDREDQYDFSDSYTTSQAVVVTKADNNDVKSEGDVKGKKAAQSLT
SNYNDLAKNAGAEIEGVEGMAQAFQLIQQGRVDLTYNDKLAVLNYLKTSGNKNLKIAFETGEPQTTHFAFRKGSGELVDK
VNSALKDMKEDGTLAKIAKKWFGEDVSK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=261, Percent_Identity=40.2298850574713, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787031, Length=220, Percent_Identity=36.3636363636364, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787085, Length=226, Percent_Identity=26.9911504424779, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=28.2700421940928, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788649, Length=273, Percent_Identity=23.4432234432234, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787088, Length=230, Percent_Identity=28.2608695652174, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29386; Mature: 29386

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAIFAVLLTLLITVMAACGASGDNSKDKNAAGDSLWSSIKDKGVLTVGTEGTYEPFTF
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEE
HDKKTDKLTGYDVEVMQEVAKRLNLKAEFKETQWDSMFAGLNSKRFDVVANQVGKKDRED
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
QYDFSDSYTTSQAVVVTKADNNDVKSEGDVKGKKAAQSLTSNYNDLAKNAGAEIEGVEGM
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHH
AQAFQLIQQGRVDLTYNDKLAVLNYLKTSGNKNLKIAFETGEPQTTHFAFRKGSGELVDK
HHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCHHHHH
VNSALKDMKEDGTLAKIAKKWFGEDVSK
HHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKAIFAVLLTLLITVMAACGASGDNSKDKNAAGDSLWSSIKDKGVLTVGTEGTYEPFTF
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEE
HDKKTDKLTGYDVEVMQEVAKRLNLKAEFKETQWDSMFAGLNSKRFDVVANQVGKKDRED
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
QYDFSDSYTTSQAVVVTKADNNDVKSEGDVKGKKAAQSLTSNYNDLAKNAGAEIEGVEGM
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHH
AQAFQLIQQGRVDLTYNDKLAVLNYLKTSGNKNLKIAFETGEPQTTHFAFRKGSGELVDK
HHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCHHHHH
VNSALKDMKEDGTLAKIAKKWFGEDVSK
HHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]