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The map label for this gene is yclA [H]

Identifier: 52784245

GI number: 52784245

Start: 425027

End: 425899

Strand: Reverse

Name: yclA [H]

Synonym: BLi00425

Alternate gene names: 52784245

Gene position: 425899-425027 (Counterclockwise)

Preceding gene: 52784250

Following gene: 52784241

Centisome position: 10.09

GC content: 50.52

Gene sequence:

>873_bases
ATGGATATTCGCCAGTTAAAATACTTCGTTACAATCGCCCGGGAAGGAAAAATTACAACGGCGGCGAAGCGGCTCCATAT
TGCACAGCCGCCGCTCTCCAGGCAGTTGAAGCAGATGGAAGAGGAGCTCGGAGTCGTTTTATTTGACCGGAACAAAAAGA
AAAACCTGACGTTGACCTACGAAGGAGAAGTTTTTTTGAAAAGAGCAAAAGAAATCCTGCACAAGCTTGAGGATTCCATC
TTGGAGGTGCGGGAGCTGCGTGAAGAAGTCAACGGAACCCTTGCCGTCGGCTCGACGATTTACTGCGCGGCCCTGCTGCT
CTCAAAAGTCTCGAAGATCAGAGATATGTATCCGAATTTAATGTTTAACATTTGGGAAGGTGAGCCTTCACGGCTAAGCG
AGCTCCTGGAAAGCCGGCAGATCGATGCAGCCGTCACGACAACGCCTATCCACAGCAAAAACACCGAATCAAAAAGCCTG
CCGGAGGTTCCATGCAAGCTTGTTCTTCCAGGGAATCAGTCGTATGGCTTAAAAGGAGAAGCCGGAATGAAAGAAATTGC
CGGCCTGCCGCTGATTCTGCTCAGGCCGTCGCACGGAAAAGGGATTTACGACGAGGTCGTCCATCACTTTCAATCATCTG
GCCTCGAACCGAGGGTCGTTTGTGAGTGCCATGATTCTACAACGCTTCTCAGCCTCGTCACTTCGGGATTCGGAGCGACG
ATTCTGCCGCTGTCCATGCTGCCGGCGCATTTTGCACGGCATGTTCAGACATTGGAAATTCAAAACAATCCGTTCATTCT
GCAGCCGTCTGTCGTTTGGAGGGCCGGCAGTTTTCTGCCGAAGCCTGTGAGAGAATTTTTGGCTTTATTTTGA

Upstream 100 bases:

>100_bases
GGTGCTTCAGTTAAAATGATACGCCTCTATTTACCATATTGAAAATATCTGTTAAATCTATATATAATACTCTAAAGGTA
TAAAAAGGAGTGCCGTGAAA

Downstream 100 bases:

>100_bases
TAAGCGCCCGGGAATGCGCCAATAAAAAAAGAGAAGGCATCATGCCGATGCCCTCTCCTTCGCATTGGTTACAGCAGTTT
CTTCCCTAACAATGATTCGA

Product: YclA

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDIRQLKYFVTIAREGKITTAAKRLHIAQPPLSRQLKQMEEELGVVLFDRNKKKNLTLTYEGEVFLKRAKEILHKLEDSI
LEVRELREEVNGTLAVGSTIYCAALLLSKVSKIRDMYPNLMFNIWEGEPSRLSELLESRQIDAAVTTTPIHSKNTESKSL
PEVPCKLVLPGNQSYGLKGEAGMKEIAGLPLILLRPSHGKGIYDEVVHHFQSSGLEPRVVCECHDSTTLLSLVTSGFGAT
ILPLSMLPAHFARHVQTLEIQNNPFILQPSVVWRAGSFLPKPVREFLALF

Sequences:

>Translated_290_residues
MDIRQLKYFVTIAREGKITTAAKRLHIAQPPLSRQLKQMEEELGVVLFDRNKKKNLTLTYEGEVFLKRAKEILHKLEDSI
LEVRELREEVNGTLAVGSTIYCAALLLSKVSKIRDMYPNLMFNIWEGEPSRLSELLESRQIDAAVTTTPIHSKNTESKSL
PEVPCKLVLPGNQSYGLKGEAGMKEIAGLPLILLRPSHGKGIYDEVVHHFQSSGLEPRVVCECHDSTTLLSLVTSGFGAT
ILPLSMLPAHFARHVQTLEIQNNPFILQPSVVWRAGSFLPKPVREFLALF
>Mature_290_residues
MDIRQLKYFVTIAREGKITTAAKRLHIAQPPLSRQLKQMEEELGVVLFDRNKKKNLTLTYEGEVFLKRAKEILHKLEDSI
LEVRELREEVNGTLAVGSTIYCAALLLSKVSKIRDMYPNLMFNIWEGEPSRLSELLESRQIDAAVTTTPIHSKNTESKSL
PEVPCKLVLPGNQSYGLKGEAGMKEIAGLPLILLRPSHGKGIYDEVVHHFQSSGLEPRVVCECHDSTTLLSLVTSGFGAT
ILPLSMLPAHFARHVQTLEIQNNPFILQPSVVWRAGSFLPKPVREFLALF

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=248, Percent_Identity=26.2096774193548, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=27.6, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=26.1016949152542, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=31.4516129032258, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=27.3092369477912, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=26.027397260274, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=25.6, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787601, Length=248, Percent_Identity=25.8064516129032, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789506, Length=291, Percent_Identity=21.3058419243986, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789204, Length=253, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=24.0157480314961, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=25.9109311740891, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=21.8623481781377, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788481, Length=244, Percent_Identity=25.8196721311475, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=142, Percent_Identity=29.5774647887324, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32520; Mature: 32520

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRQLKYFVTIAREGKITTAAKRLHIAQPPLSRQLKQMEEELGVVLFDRNKKKNLTLTY
CCHHHEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEE
EGEVFLKRAKEILHKLEDSILEVRELREEVNGTLAVGSTIYCAALLLSKVSKIRDMYPNL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCHHH
MFNIWEGEPSRLSELLESRQIDAAVTTTPIHSKNTESKSLPEVPCKLVLPGNQSYGLKGE
EEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
AGMKEIAGLPLILLRPSHGKGIYDEVVHHFQSSGLEPRVVCECHDSTTLLSLVTSGFGAT
CCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCHH
ILPLSMLPAHFARHVQTLEIQNNPFILQPSVVWRAGSFLPKPVREFLALF
HHHHHHHHHHHHHHHEEEEECCCCEEECCHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MDIRQLKYFVTIAREGKITTAAKRLHIAQPPLSRQLKQMEEELGVVLFDRNKKKNLTLTY
CCHHHEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEE
EGEVFLKRAKEILHKLEDSILEVRELREEVNGTLAVGSTIYCAALLLSKVSKIRDMYPNL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCHHH
MFNIWEGEPSRLSELLESRQIDAAVTTTPIHSKNTESKSLPEVPCKLVLPGNQSYGLKGE
EEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
AGMKEIAGLPLILLRPSHGKGIYDEVVHHFQSSGLEPRVVCECHDSTTLLSLVTSGFGAT
CCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCHH
ILPLSMLPAHFARHVQTLEIQNNPFILQPSVVWRAGSFLPKPVREFLALF
HHHHHHHHHHHHHHHEEEEECCCCEEECCHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]