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The map label for this gene is yckJ [H]

Identifier: 52784239

GI number: 52784239

Start: 417973

End: 418677

Strand: Reverse

Name: yckJ [H]

Synonym: BLi00419

Alternate gene names: 52784239

Gene position: 418677-417973 (Counterclockwise)

Preceding gene: 52784240

Following gene: 52784238

Centisome position: 9.92

GC content: 47.94

Gene sequence:

>705_bases
ATGTTTCTAAGTAATATACAAGCGGCGGCCATCGGCCAGGCGATCCCGTGGGATCTTGTGCGTGAGTCATTTTGGCCGAT
CTTTTTAAGGGGAATCTATTACTCGATTCCCCTTGCCATTCTCTCTTTTATATTCGGTATGATCATCGCATTGATCACGG
CGCTGGCGAGAATGTCCAAATCCCGCATTTTAAGAGCGGTTTTCGGCATTTATGTCTCAGCGATCCGCGGAACGCCGCTG
CTCGTTCAGCTGTTCATTATTTTTTACTTGTTTCCAGCGTTTCAAGTCACGATCGACCCGTTTCCGAGCGCAATCATTGC
CTTTTCATTAAATGTCGGCGCTTATGCGTCGGAAATCATCAGGGCTTCCATCCTTTCCGTTCCGAAAGGACAGTGGGAGG
CGGCTTATTCGATTGGAATGACTCAAAAAACGGCGCTTACCAGGGTCATCCTGCCGCAGGCTGTGCGCGTGTCAATTCCG
CCATTATCCAATACATTCATCAGCTTGGTTAAAGATACATCGCTTGCGTCACAGATTCTTGTTGCTGAATTATTCCGGAA
GGCCCAGGAAATCGGGGCCGGCAATCTTGACCAAATTTTAATCATTTATATGGAAGCCGCATTCATCTATTGGATCATCT
GCTTTGTTCTCGCGCTTGTTCAGCAGCAGATTGAACGGCGTTTGGACCGGTATGTGGCCAAGTAA

Upstream 100 bases:

>100_bases
AAAGGAAGCGGCGAACTGGTTGACAAGGTCAACTCCGCGCTGAAGGACATGAAAGAGGATGGCACACTCGCGAAAATAGC
GAAGAAATGGTTTGGCGAAG

Downstream 100 bases:

>100_bases
GGAGGGAAATAGACCATGCTTGCGATCAAAGGGCTGAATAAATCCTTCGGCGAAAATGAAATCTTAAAAAAAATCGATCT
GAACATCGAAAAAGGAAAAG

Product: YckJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFLSNIQAAAIGQAIPWDLVRESFWPIFLRGIYYSIPLAILSFIFGMIIALITALARMSKSRILRAVFGIYVSAIRGTPL
LVQLFIIFYLFPAFQVTIDPFPSAIIAFSLNVGAYASEIIRASILSVPKGQWEAAYSIGMTQKTALTRVILPQAVRVSIP
PLSNTFISLVKDTSLASQILVAELFRKAQEIGAGNLDQILIIYMEAAFIYWIICFVLALVQQQIERRLDRYVAK

Sequences:

>Translated_234_residues
MFLSNIQAAAIGQAIPWDLVRESFWPIFLRGIYYSIPLAILSFIFGMIIALITALARMSKSRILRAVFGIYVSAIRGTPL
LVQLFIIFYLFPAFQVTIDPFPSAIIAFSLNVGAYASEIIRASILSVPKGQWEAAYSIGMTQKTALTRVILPQAVRVSIP
PLSNTFISLVKDTSLASQILVAELFRKAQEIGAGNLDQILIIYMEAAFIYWIICFVLALVQQQIERRLDRYVAK
>Mature_234_residues
MFLSNIQAAAIGQAIPWDLVRESFWPIFLRGIYYSIPLAILSFIFGMIIALITALARMSKSRILRAVFGIYVSAIRGTPL
LVQLFIIFYLFPAFQVTIDPFPSAIIAFSLNVGAYASEIIRASILSVPKGQWEAAYSIGMTQKTALTRVILPQAVRVSIP
PLSNTFISLVKDTSLASQILVAELFRKAQEIGAGNLDQILIIYMEAAFIYWIICFVLALVQQQIERRLDRYVAK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788226, Length=205, Percent_Identity=50.2439024390244, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787030, Length=206, Percent_Identity=38.3495145631068, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787086, Length=215, Percent_Identity=31.1627906976744, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI87082239, Length=185, Percent_Identity=35.6756756756757, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788646, Length=209, Percent_Identity=29.6650717703349, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786873, Length=210, Percent_Identity=32.3809523809524, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788645, Length=212, Percent_Identity=30.188679245283, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786874, Length=211, Percent_Identity=27.9620853080569, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI48994930, Length=223, Percent_Identity=30.9417040358744, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26142; Mature: 26142

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSNIQAAAIGQAIPWDLVRESFWPIFLRGIYYSIPLAILSFIFGMIIALITALARMSK
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRILRAVFGIYVSAIRGTPLLVQLFIIFYLFPAFQVTIDPFPSAIIAFSLNVGAYASEII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCHHHHHHHHH
RASILSVPKGQWEAAYSIGMTQKTALTRVILPQAVRVSIPPLSNTFISLVKDTSLASQIL
HHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHH
VAELFRKAQEIGAGNLDQILIIYMEAAFIYWIICFVLALVQQQIERRLDRYVAK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLSNIQAAAIGQAIPWDLVRESFWPIFLRGIYYSIPLAILSFIFGMIIALITALARMSK
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRILRAVFGIYVSAIRGTPLLVQLFIIFYLFPAFQVTIDPFPSAIIAFSLNVGAYASEII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCHHHHHHHHH
RASILSVPKGQWEAAYSIGMTQKTALTRVILPQAVRVSIPPLSNTFISLVKDTSLASQIL
HHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHH
VAELFRKAQEIGAGNLDQILIIYMEAAFIYWIICFVLALVQQQIERRLDRYVAK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]