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The map label for this gene is radA [H]

Identifier: 52783943

GI number: 52783943

Start: 104859

End: 106238

Strand: Direct

Name: radA [H]

Synonym: BLi00105

Alternate gene names: 52783943

Gene position: 104859-106238 (Clockwise)

Preceding gene: 52783942

Following gene: 52783944

Centisome position: 2.48

GC content: 48.91

Gene sequence:

>1380_bases
ATGGCTAAAACAAAGACTAAATTCATTTGTCACTCATGCGGCTACGAATCCGCCAAGTGGATGGGGAAATGCCCCGGATG
CGGTGCATGGAATACCATGGTGGAAGAAACGATAAAAAAACCCGCCGCCAACAGAAGAACCGCTTTTTCACATTCCGTTC
AAACGGTGCAAAAGCCTTCACCTCTCACTTCAATTGAAACAACAGAAGAGCCGCGAATTAAAACGAAACTTGGCGAATTC
AACCGCGTCTTGGGAAACGGCATCGTCAAAGGTTCACTTGTTTTAATCGGAGGCGACCCCGGCATCGGGAAATCAACCTT
GCTGCTTCAAGTATCTGCCCAGCTCGCTGATTCAAAAGAAAATGTCCTGTACATCTCAGGCGAAGAATCGGTCAAGCAGA
CAAAGCTGAGAGCAGACCGTCTAGGTATCAACAGTCCCACTCTTCACGTTTTATCTGAAACCGATATGGAGTATATTACG
TCTTCTATAGAAGAGATGAACCCATCATTCGTAGTGGTTGATTCGATTCAAACCGTTTACCAAAGTGATATCACATCCGC
TCCGGGCAGCGTCTCCCAGGTCAGGGAATGCACCGCTGAGCTGATGAGAATTGCAAAAACGAAAGGGATTCCAATATTTA
TCGTCGGGCATGTCACAAAAGAAGGTTCGATTGCCGGACCGAGACTTCTGGAACATATGGTCGACACCGTCCTTTACTTT
GAAGGTGAGCGGCATCATACATTTCGGATTTTAAGAGCCGTCAAAAACCGGTTTGGATCAACGAATGAAATGGGAATCTT
TGAAATGAGGGAAGAGGGCCTGACAGAGGTGCTGAATCCGTCAGAAATCTTTCTCGAAGAGCGCTCGGCGGGAGCGGCCG
GATCGAGTGTCGTGGCCTCAATGGAAGGCACGAGGCCAGTCTTAGTAGAGATTCAGGCGCTGATTTCCCCCACGAGCTTT
GGGAATCCGCGCCGGATGGCAACCGGGATTGATCATAACCGCGTCTCATTGCTAATGGCAGTTTTAGAAAAAAGGGTTGG
ACTGCTGCTGCAAAATCAAGACGCCTATTTAAAAGTCGCCGGAGGCGTCAAGCTGGACGAGCCAGCGATTGACCTCGCCG
TTGCAGTGAGCATCGCCTCAAGCTTCAGAGACACCCCGCCTCATCCGACGGATTGTTTTATCGGAGAAGTCGGATTGACA
GGGGAGGTCCGCAGAGTATCAAGGATAGAACAGAGGGTGCAGGAAGCGGCGAAGCTTGGTTTTAAAAGAATGATTATTCC
TTCTGCAAATGTAGAAGGATGGACAAAGCCGAAAGGAATCGAAGTCGTCGGCGTTGAAAATGTAGCTGAGGCCCTTCGAG
CTTCATTAGGAGGATCATAA

Upstream 100 bases:

>100_bases
ACCGAAGCTAAAACGAACTAATATAGAGACAAAAGAAAGAGGCATACGATGTTCATCAGTATGCCTCACTTTCTTATAAA
GGGAGCGTTTTGAAAAATCA

Downstream 100 bases:

>100_bases
TATATGGACAAAGACAAAAAAGGAGCGAAACAGGATCTTTCAGAAATATTGCAGTTTGTCGCCCCCGGCACGCCTCTTCG
CGAAGGAATCGAAAACGTCC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAKTKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQTVQKPSPLTSIETTEEPRIKTKLGEF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKENVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS

Sequences:

>Translated_459_residues
MAKTKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQTVQKPSPLTSIETTEEPRIKTKLGEF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKENVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS
>Mature_458_residues
AKTKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQTVQKPSPLTSIETTEEPRIKTKLGEFN
RVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKENVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYITS
SIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYFE
GERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSFG
NPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLTG
EVRRVSRIEQRVQEAAKLGFKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=457, Percent_Identity=47.2647702407002, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49819; Mature: 49688

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQTVQKPS
CCCCCHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
PLTSIETTEEPRIKTKLGEFNRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKE
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEECEEEEEECCCCCCCCEEEEEEEHHHCCCCC
NVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYITSSIEEMNPSFVVVDSIQTVY
CEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEHHHHHHH
QSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVAS
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHEEE
MEGTRPVLVEIQALISPTSFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVA
CCCCCCCEEEHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCEEEEE
GGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLTGEVRRVSRIEQRVQEAAKLG
CCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
FKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS
CCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKTKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQTVQKPS
CCCCHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
PLTSIETTEEPRIKTKLGEFNRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKE
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEECEEEEEECCCCCCCCEEEEEEEHHHCCCCC
NVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYITSSIEEMNPSFVVVDSIQTVY
CEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEHHHHHHH
QSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVAS
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHEEE
MEGTRPVLVEIQALISPTSFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVA
CCCCCCCEEEHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCEEEEE
GGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLTGEVRRVSRIEQRVQEAAKLG
CCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
FKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS
CCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]