Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 52142904

GI number: 52142904

Start: 2468307

End: 2469380

Strand: Direct

Name: murE [H]

Synonym: BCZK2337

Alternate gene names: 52142904

Gene position: 2468307-2469380 (Clockwise)

Preceding gene: 52142903

Following gene: 52142901

Centisome position: 46.56

GC content: 32.22

Gene sequence:

>1074_bases
ATGGGGAATAACGGAGTGAAAGTTGGTTCAAAATGGTATCCTACTGATATTAATACGCAAGAACCTCCAACACTTCAACG
GAATTTGCAAATGATGAAAAATCGAAATGCAGATTATTGTGTTATGGAAGTAACGTCACAAGGTTTACATATGGAAAGAG
TGAAAGGATGTAACTTTAAAATAGCTGTTTTCACAAATTTAACGCAAGATCATTTGGATTATCATGGAACATTTCTTGAA
TATAAGCATGTAAAAGGTCTTCTTTTTGCAAGATTAGGAAATGATTTATCTACTACAGAGAAAAAAATTGCTGTTTTGAA
TGCAGACGATCCATCGGTAGAATACTTTAAAAAAATTACTTCAGCTGAGGTTATCACATATGGAATACATAATAGAGCAG
ATGTTCAAGCGAAGAACATAAATTTAAGCCCAAAAGGAATTCGTTTTTTAGTTTCGTCCTTTAATGGAGAAATAGAGGTT
AGCCTTAATCTTGTAGGTCGTTTTAATATTTATAATGCATTAGCAGCAATAACAGTTGCAATAGTTGAAGGTATTCCGCT
TACAAACATTTGTGATAGCTTATCAAACTTAAAGAGTATTGAAGGTAGAATGGAAATCATAGATGAAAATCAAGATTTTT
TTGTAATAGTGGACTATGCTCATACACCGGATGCATTAGAAAATGTTCTCTCTACAATTAGCGAGTTTTCAAAAGGTAAA
GTTATTACTGTTTTCGGTTGTGGTGGAGATAGAGACGCAAAAAAACGATCTATAATGGGCGGAATTGCTGGAAGTTATAG
TGATTTTGTTTTTATTACTTCTGATAATCCACGTTCGGAAGATCCGCAGGCAATAATGAAAGATATTGAAAAAGGATTTT
CGCAAAATAATAATTTGAATTATAAAGTGGAAGTAGATCGTGAACTTGCAATAAATCACGCAATAAACATGGCATCTTCT
AATGATATTGTTTTGATTGCAGGGAAAGGGCATGAAACGTATCAAATTTTAAAAGATTCAACTATTCATTTTGATGATAA
AGAAAAAGCTCGACAAGCAATTATTAATAAATGA

Upstream 100 bases:

>100_bases
TTACAGGTACAAATGGGAAAACAACTACTTCCTATTTACTTGAAAAAATTTTAAGTGATTATGGATATCACACGGGGCTT
ATGGATATCACACGGGGCTT

Downstream 100 bases:

>100_bases
ATGAATTCAATTACTAGCTGAATAGTATTTCTAATAAAAATAATGTTTTAGATGAAGTAATAACGAAGCAATTTTTTGAA
GAATTTATAATTGAAAGGTA

Product: UDP-N-acetylmuramoylananine-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MGNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNRNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFLE
YKHVKGLLFARLGNDLSTTEKKIAVLNADDPSVEYFKKITSAEVITYGIHNRADVQAKNINLSPKGIRFLVSSFNGEIEV
SLNLVGRFNIYNALAAITVAIVEGIPLTNICDSLSNLKSIEGRMEIIDENQDFFVIVDYAHTPDALENVLSTISEFSKGK
VITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAIMKDIEKGFSQNNNLNYKVEVDRELAINHAINMASS
NDIVLIAGKGHETYQILKDSTIHFDDKEKARQAIINK

Sequences:

>Translated_357_residues
MGNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNRNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFLE
YKHVKGLLFARLGNDLSTTEKKIAVLNADDPSVEYFKKITSAEVITYGIHNRADVQAKNINLSPKGIRFLVSSFNGEIEV
SLNLVGRFNIYNALAAITVAIVEGIPLTNICDSLSNLKSIEGRMEIIDENQDFFVIVDYAHTPDALENVLSTISEFSKGK
VITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAIMKDIEKGFSQNNNLNYKVEVDRELAINHAINMASS
NDIVLIAGKGHETYQILKDSTIHFDDKEKARQAIINK
>Mature_356_residues
GNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNRNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFLEY
KHVKGLLFARLGNDLSTTEKKIAVLNADDPSVEYFKKITSAEVITYGIHNRADVQAKNINLSPKGIRFLVSSFNGEIEVS
LNLVGRFNIYNALAAITVAIVEGIPLTNICDSLSNLKSIEGRMEIIDENQDFFVIVDYAHTPDALENVLSTISEFSKGKV
ITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAIMKDIEKGFSQNNNLNYKVEVDRELAINHAINMASSN
DIVLIAGKGHETYQILKDSTIHFDDKEKARQAIINK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=356, Percent_Identity=37.9213483146067, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 39782; Mature: 39650

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNRNADYCVMEVTSQGLHMERVKGCNFK
CCCCCEEECCEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCEE
IAVFTNLTQDHLDYHGTFLEYKHVKGLLFARLGNDLSTTEKKIAVLNADDPSVEYFKKIT
EEEEECCCHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHC
SAEVITYGIHNRADVQAKNINLSPKGIRFLVSSFNGEIEVSLNLVGRFNIYNALAAITVA
CCEEEEEECCCCCCEEEECCCCCCHHHHHHEECCCCEEEEEEEEEEEHHHHHHHHHHHHH
IVEGIPLTNICDSLSNLKSIEGRMEIIDENQDFFVIVDYAHTPDALENVLSTISEFSKGK
HHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
VITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAIMKDIEKGFSQNNNLN
EEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCE
YKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFDDKEKARQAIINK
EEEEECHHHHHHHHEEECCCCCEEEEECCCCCEEEEECCCEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
GNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNRNADYCVMEVTSQGLHMERVKGCNFK
CCCCEEECCEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCEE
IAVFTNLTQDHLDYHGTFLEYKHVKGLLFARLGNDLSTTEKKIAVLNADDPSVEYFKKIT
EEEEECCCHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHC
SAEVITYGIHNRADVQAKNINLSPKGIRFLVSSFNGEIEVSLNLVGRFNIYNALAAITVA
CCEEEEEECCCCCCEEEECCCCCCHHHHHHEECCCCEEEEEEEEEEEHHHHHHHHHHHHH
IVEGIPLTNICDSLSNLKSIEGRMEIIDENQDFFVIVDYAHTPDALENVLSTISEFSKGK
HHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
VITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAIMKDIEKGFSQNNNLN
EEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCE
YKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFDDKEKARQAIINK
EEEEECHHHHHHHHEEECCCCCEEEEECCCCCEEEEECCCEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA