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The map label for this gene is murE [H]

Identifier: 52142903

GI number: 52142903

Start: 2467882

End: 2468325

Strand: Direct

Name: murE [H]

Synonym: BCZK2336

Alternate gene names: 52142903

Gene position: 2467882-2468325 (Clockwise)

Preceding gene: 52142908

Following gene: 52142904

Centisome position: 46.56

GC content: 33.33

Gene sequence:

>444_bases
TTGAAACTAAAAGAGCTGGCGAATTTGTTATTGGTAAAAGAAACTATTGGCGATATGAATGTAAAAATTACGGGATTAGA
AATGGATTCTAGAAAGATAACTAATGGAAACCTATTTATATGTGTATCAGGTATCGATGGTTTTCTTGAAGATCGACATC
AATTTGTTGAAGATGCAGTGAAAAATGGTGCTGTAGCACTAATTGTAGAAAGAGATGTAAATATTGAGATACCTAAAATA
ATTGTTAAAGATGCTAGGTATGCTATGGCAGTTATTGCGTCACATTTCTATGGTTATCCATCTAATAAAATGAAGCTAAT
TGGAATTACAGGTACAAATGGGAAAACAACTACTTCCTATTTACTTGAAAAAATTTTAAGTGATTATGGATATCACACGG
GGCTTATGGATATCACACGGGGCTTATGGGGAATAACGGAGTGA

Upstream 100 bases:

>100_bases
AAGGGTGATTAAATTTAGTTCAAAAAATATGGAATTTTAAACCATTGTTTTTATACAAAGTGGTAAACTTAATAAGGTAA
TATGTAAGGGGGCGGGGGAT

Downstream 100 bases:

>100_bases
AAGTTGGTTCAAAATGGTATCCTACTGATATTAATACGCAAGAACCTCCAACACTTCAACGGAATTTGCAAATGATGAAA
AATCGAAATGCAGATTATTG

Product: UDP-N-acetylmuramoylananine-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLLEKILSDYGYHTGLMDITRGLWGITE

Sequences:

>Translated_147_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLLEKILSDYGYHTGLMDITRGLWGITE
>Mature_147_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLLEKILSDYGYHTGLMDITRGLWGITE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 16327; Mature: 16327

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAV
CCHHHHHHHHHHHHHCCCCEEEEEEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHH
KNGAVALIVERDVNIEIPKIIVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY
CCCEEEEEEECCCCEECCHHEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHH
LLEKILSDYGYHTGLMDITRGLWGITE
HHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAV
CCHHHHHHHHHHHHHCCCCEEEEEEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHH
KNGAVALIVERDVNIEIPKIIVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY
CCCEEEEEEECCCCEECCHHEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHH
LLEKILSDYGYHTGLMDITRGLWGITE
HHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]