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The map label for this gene is rocG [H]

Identifier: 51893943

GI number: 51893943

Start: 3017205

End: 3018521

Strand: Reverse

Name: rocG [H]

Synonym: STH2805

Alternate gene names: 51893943

Gene position: 3018521-3017205 (Counterclockwise)

Preceding gene: 51893944

Following gene: 51893942

Centisome position: 84.64

GC content: 69.86

Gene sequence:

>1317_bases
GTGAGAGGCGTCACGAATGGGGGGAAAGAGATGGGCGGCGCTCAAAATCCGTTTCTGATTGCGCAACAGCAGGTTCGGAA
CGCGGTGGCGGCGTTGGGCGAGAGCGAGGAGCTGTTCGAGCTCCTGAAGGCGCCGGCCCATTTCATCGAGGTGCAGATCC
CGGTGCGCATGGATGACGGGAGCCTGCGCGTGTTCACCGGGTACCGGAGCCAGCACCTCACGACCCTCGGGCCCGCCAAG
GGAGGCATCCGGTTCCACCCGGCGGTCACCGCCGATGAGGTGAAGGCCCTCTCCATGTGGATGACGTTCAAGACGTCCGT
GGTGGGGCTCCCCTACGGCGGCGGCAAGGGCGGCGTCGTCGTCGACCCGCGCAAGCTCAGCCTGGGCGAACTGGAGCGGC
TCTCCCGCGGCTACGTGCGGGCGATCTGGCCCTACCTCGGGCCCGACAAGGACATTCCTGCCCCAGACGTGAACACCAAC
GCGCAGATCATGGGCTGGATGACCGACGAGTACGAGACCATCGTGGGCGCCAGCTGTCCGGCCGTGTTCACGGGCAAGCC
GCTCTCCATGGGCGGCTCGCTGGGCCGCGAGGAGGCCACCGGCCGGGGCACCGTCATCACCATCCGCGAGGCCCTGGCGT
ACCTGGGGCTGCCCGTGGCGGGCGCCCGGGTGGCGGTGCAGGGCTTCGGCAACGCCGGACAGCACGCGGCGCGGCTGCTG
GAGGAGCTGGGAGCCGTCCTGGTCGCGGCCAGCGACAGCCGTGGCGGCATCGTCTGCCCCGGCGGAATGCGCGTGGCCGA
CCTCATCGCGTACAAGCAGGCGACGGGCAGCGTCGTCGGCTTCCCTGGCAGCCGGGCCACCGACCAGTTCGGGGTGCTGA
CGGCCGACTGCGACATCCTGGTGCCGGCGGCCCTGGAGAACCAGATCGACGCCGAGGTGGCCAGGTCCATCCGGGCCCGC
GTGGTGGCCGAGGCGGCCAACGGCCCGACGACGCCCGACGGTGACCACGTCCTGGCGCAGCGGAAGATTTTTCTCATCCC
GGACATCCTCGCCAACGCAGGCGGCGTGACCGTTTCGTATTTTGAGTGGGTGCAGAACCGGAACCAGTATTACTGGACGG
CGGAGGAGGTCAACGCCCGGCTGGAACAGCGCATGGTGGCCTCCTTCCGCGACGTGGTCGCCATGGCCGAGCGGCACGGT
GCCTACACCCGGACGGCGGCCTACATGTACGCCATCCACCGCATCGCCGAATCGCTGCGGATGCGGGGGCTGCTCAGGGC
GCCGGCCCGCCAGGCGCTCCGGGCGGCGGCCGGCTAA

Upstream 100 bases:

>100_bases
GGCGGCTTCGCCTTATTCGCTGTTCAAATCGATTTGATTCGTTTTTTTGTCTTGGCACAGCCCCTTTCCCTCCGCTATAC
TAAAGCCCTAAAGGCATAAA

Downstream 100 bases:

>100_bases
TCGGCCGGAACCTGCGCATATGCTGGAGCCGTCCCTCCCGGGCTCTGCCGGAGGGACGGTTTTTTGCGCGGGCGACGGGA
TTCGGCGGCGCAGCGGCGAA

Product: glutamate/leucine dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRGVTNGGKEMGGAQNPFLIAQQQVRNAVAALGESEELFELLKAPAHFIEVQIPVRMDDGSLRVFTGYRSQHLTTLGPAK
GGIRFHPAVTADEVKALSMWMTFKTSVVGLPYGGGKGGVVVDPRKLSLGELERLSRGYVRAIWPYLGPDKDIPAPDVNTN
AQIMGWMTDEYETIVGASCPAVFTGKPLSMGGSLGREEATGRGTVITIREALAYLGLPVAGARVAVQGFGNAGQHAARLL
EELGAVLVAASDSRGGIVCPGGMRVADLIAYKQATGSVVGFPGSRATDQFGVLTADCDILVPAALENQIDAEVARSIRAR
VVAEAANGPTTPDGDHVLAQRKIFLIPDILANAGGVTVSYFEWVQNRNQYYWTAEEVNARLEQRMVASFRDVVAMAERHG
AYTRTAAYMYAIHRIAESLRMRGLLRAPARQALRAAAG

Sequences:

>Translated_438_residues
MRGVTNGGKEMGGAQNPFLIAQQQVRNAVAALGESEELFELLKAPAHFIEVQIPVRMDDGSLRVFTGYRSQHLTTLGPAK
GGIRFHPAVTADEVKALSMWMTFKTSVVGLPYGGGKGGVVVDPRKLSLGELERLSRGYVRAIWPYLGPDKDIPAPDVNTN
AQIMGWMTDEYETIVGASCPAVFTGKPLSMGGSLGREEATGRGTVITIREALAYLGLPVAGARVAVQGFGNAGQHAARLL
EELGAVLVAASDSRGGIVCPGGMRVADLIAYKQATGSVVGFPGSRATDQFGVLTADCDILVPAALENQIDAEVARSIRAR
VVAEAANGPTTPDGDHVLAQRKIFLIPDILANAGGVTVSYFEWVQNRNQYYWTAEEVNARLEQRMVASFRDVVAMAERHG
AYTRTAAYMYAIHRIAESLRMRGLLRAPARQALRAAAG
>Mature_438_residues
MRGVTNGGKEMGGAQNPFLIAQQQVRNAVAALGESEELFELLKAPAHFIEVQIPVRMDDGSLRVFTGYRSQHLTTLGPAK
GGIRFHPAVTADEVKALSMWMTFKTSVVGLPYGGGKGGVVVDPRKLSLGELERLSRGYVRAIWPYLGPDKDIPAPDVNTN
AQIMGWMTDEYETIVGASCPAVFTGKPLSMGGSLGREEATGRGTVITIREALAYLGLPVAGARVAVQGFGNAGQHAARLL
EELGAVLVAASDSRGGIVCPGGMRVADLIAYKQATGSVVGFPGSRATDQFGVLTADCDILVPAALENQIDAEVARSIRAR
VVAEAANGPTTPDGDHVLAQRKIFLIPDILANAGGVTVSYFEWVQNRNQYYWTAEEVNARLEQRMVASFRDVVAMAERHG
AYTRTAAYMYAIHRIAESLRMRGLLRAPARQALRAAAG

Specific function: Catabolic GlutDH involved in the utilization of glutamate and other amino acids of the glutamate family [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=363, Percent_Identity=40.771349862259, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI4885281, Length=362, Percent_Identity=40.8839779005525, Blast_Score=281, Evalue=6e-76,
Organism=Escherichia coli, GI1788059, Length=408, Percent_Identity=33.3333333333333, Blast_Score=200, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17544676, Length=365, Percent_Identity=38.6301369863014, Blast_Score=254, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6319256, Length=427, Percent_Identity=30.2107728337237, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6324951, Length=417, Percent_Identity=30.9352517985612, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45553475, Length=452, Percent_Identity=35.8407079646018, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI45549226, Length=345, Percent_Identity=40.2898550724638, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649283, Length=345, Percent_Identity=37.6811594202899, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24649460, Length=282, Percent_Identity=38.6524822695035, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46821; Mature: 46821

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGVTNGGKEMGGAQNPFLIAQQQVRNAVAALGESEELFELLKAPAHFIEVQIPVRMDDG
CCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEEEECCC
SLRVFTGYRSQHLTTLGPAKGGIRFHPAVTADEVKALSMWMTFKTSVVGLPYGGGKGGVV
CEEEEECCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHEEHHEEEECCCCCCCCCEE
VDPRKLSLGELERLSRGYVRAIWPYLGPDKDIPAPDVNTNAQIMGWMTDEYETIVGASCP
ECCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCC
AVFTGKPLSMGGSLGREEATGRGTVITIREALAYLGLPVAGARVAVQGFGNAGQHAARLL
EEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
EELGAVLVAASDSRGGIVCPGGMRVADLIAYKQATGSVVGFPGSRATDQFGVLTADCDIL
HHCCCEEEEECCCCCCEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCEE
VPAALENQIDAEVARSIRARVVAEAANGPTTPDGDHVLAQRKIFLIPDILANAGGVTVSY
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEHHHHCCCCCCCHHH
FEWVQNRNQYYWTAEEVNARLEQRMVASFRDVVAMAERHGAYTRTAAYMYAIHRIAESLR
HHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MRGLLRAPARQALRAAAG
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRGVTNGGKEMGGAQNPFLIAQQQVRNAVAALGESEELFELLKAPAHFIEVQIPVRMDDG
CCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEEEECCC
SLRVFTGYRSQHLTTLGPAKGGIRFHPAVTADEVKALSMWMTFKTSVVGLPYGGGKGGVV
CEEEEECCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHEEHHEEEECCCCCCCCCEE
VDPRKLSLGELERLSRGYVRAIWPYLGPDKDIPAPDVNTNAQIMGWMTDEYETIVGASCP
ECCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCC
AVFTGKPLSMGGSLGREEATGRGTVITIREALAYLGLPVAGARVAVQGFGNAGQHAARLL
EEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
EELGAVLVAASDSRGGIVCPGGMRVADLIAYKQATGSVVGFPGSRATDQFGVLTADCDIL
HHCCCEEEEECCCCCCEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCEE
VPAALENQIDAEVARSIRARVVAEAANGPTTPDGDHVLAQRKIFLIPDILANAGGVTVSY
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEHHHHCCCCCCCHHH
FEWVQNRNQYYWTAEEVNARLEQRMVASFRDVVAMAERHGAYTRTAAYMYAIHRIAESLR
HHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MRGLLRAPARQALRAAAG
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 7565595; 9829940 [H]