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The map label for this gene is murD [H]

Identifier: 51598838

GI number: 51598838

Start: 604334

End: 605689

Strand: Direct

Name: murD [H]

Synonym: BG0598

Alternate gene names: 51598838

Gene position: 604334-605689 (Clockwise)

Preceding gene: 51598837

Following gene: 51598841

Centisome position: 66.83

GC content: 28.17

Gene sequence:

>1356_bases
GTGCGTTTAGACGAAATTAAAAATTTAAATTTTTTAGTCATGGGATTAGGTCTTAATGGAGGGGGAGTAGCTCTTTCTAG
ATTTTTATTAAAGCACGGGGCAAAGCTAGTAATTACTGATCTTAAAAGTGAAGCAGAATTAGCTTTAAGTATTGATTCTT
TAAAAGATTTTGATGATCAAATTAGGTACGTTTTAGGTAAACATGATATAAACGATTTTAAAAAAGCTGATATTGTTGTT
AAAAATCCTAGTGTTAAGCCTAATAATAAATATTTAAAGCTTGCAAAACGAGTTGAAACTGATATTAGCTTATTTTTGAT
ATTCAACAAGAACCCTATAATTGCAGTAACAGGCACTAAAGGTAAATCAACTCTTGTATCTCTTTTGTATCAGGTTTTGA
AAAAAAATTATCCAAGGGCAAAGCTTGGAGGAAATATCGGTGTATCTCCTTTAAGTTTTTTTGATCAACTTGATGGAATA
TCTCCCTTGATTTTAGAGCTTTCTTCTTGGCAGTTGCAATCTTTAGAGAATTTTAATCCTATTCTTAGTATTATTACAAA
TGTTTACAACGATCACCAAAACTATTATTTAAATTTTGATGACTATATTATTGATAAGTCAAAGATTTTTGTAAATCAAG
CTTCAGGAATTACGATTATTCAGGATAAGGCTTATTACAAATATTTTTCAAAATTTGAATCAAAAGCCAAAGTTATTTTA
TTTTCCGAATTTAATCCTTTCGTTTTAGATCAAGATGTTTTTTATTATAATAAAGGCGAAGTATATTTTAATGACAACTT
GATTGGAAGTTTTTCTGAGTCACAAGTTGTTTTTATGATTCCCAAGCTTATTACTTTTTTTGTTTCGTATTATTTAAATA
TGGACCTTAATCATACGTTTAAGATTTTAAATAAGTTTAGAGGTATTGAGCATAGAGTAGAGTTTGTTAAATTAGTTCAA
AATGTAATGTTTTATAATGATACAGCTTCAACTATTCCCGATTCTACGGTTTTATCTGTTAAAAGTTTAAAAACAAATGA
TAATTCTATTAACTTAATTGCTGGAGGAACTGATAAAGAGCTTGATTTTTCAAGTTTTCGTAAGATCATTAATCTTGTGA
AAACTTGGATCTTGATCAAAGGTAGTGCAACTGTAAAAATTATTAAGATTTTAGAAAAAAGTAGCATACAATATTTTGTA
TTTGATTCTTTAAGAGATGCAGTAAATTATGCTATCAAGATTTCAAGTCCAGGTGACATTGTATTATTTTCCCCCGCCAG
TGCTTCTTTTGAGCTTTTCAATAATGAGTTTGATAGAGGCTTACAATTCAAAAATTTAGTTAATATGCTTGGTTAA

Upstream 100 bases:

>100_bases
TGAGGATGCAATAAAACTTGCTATTTCGCTTCCTTATTTTTATAGTGAAAGGTGGATTAAACCTGTTAAAACAAGTTGGT
GGGTTTGATATAATCTTATT

Downstream 100 bases:

>100_bases
ATTTTTTTCCTTATTACTTTGTAATAAAAGTATCTGAGTTTTTCAAGATTTGCATAAATCTGATAAATAAAATTTTTGTA
AATAAAATCATAGCATCCAA

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRLDEIKNLNFLVMGLGLNGGGVALSRFLLKHGAKLVITDLKSEAELALSIDSLKDFDDQIRYVLGKHDINDFKKADIVV
KNPSVKPNNKYLKLAKRVETDISLFLIFNKNPIIAVTGTKGKSTLVSLLYQVLKKNYPRAKLGGNIGVSPLSFFDQLDGI
SPLILELSSWQLQSLENFNPILSIITNVYNDHQNYYLNFDDYIIDKSKIFVNQASGITIIQDKAYYKYFSKFESKAKVIL
FSEFNPFVLDQDVFYYNKGEVYFNDNLIGSFSESQVVFMIPKLITFFVSYYLNMDLNHTFKILNKFRGIEHRVEFVKLVQ
NVMFYNDTASTIPDSTVLSVKSLKTNDNSINLIAGGTDKELDFSSFRKIINLVKTWILIKGSATVKIIKILEKSSIQYFV
FDSLRDAVNYAIKISSPGDIVLFSPASASFELFNNEFDRGLQFKNLVNMLG

Sequences:

>Translated_451_residues
MRLDEIKNLNFLVMGLGLNGGGVALSRFLLKHGAKLVITDLKSEAELALSIDSLKDFDDQIRYVLGKHDINDFKKADIVV
KNPSVKPNNKYLKLAKRVETDISLFLIFNKNPIIAVTGTKGKSTLVSLLYQVLKKNYPRAKLGGNIGVSPLSFFDQLDGI
SPLILELSSWQLQSLENFNPILSIITNVYNDHQNYYLNFDDYIIDKSKIFVNQASGITIIQDKAYYKYFSKFESKAKVIL
FSEFNPFVLDQDVFYYNKGEVYFNDNLIGSFSESQVVFMIPKLITFFVSYYLNMDLNHTFKILNKFRGIEHRVEFVKLVQ
NVMFYNDTASTIPDSTVLSVKSLKTNDNSINLIAGGTDKELDFSSFRKIINLVKTWILIKGSATVKIIKILEKSSIQYFV
FDSLRDAVNYAIKISSPGDIVLFSPASASFELFNNEFDRGLQFKNLVNMLG
>Mature_451_residues
MRLDEIKNLNFLVMGLGLNGGGVALSRFLLKHGAKLVITDLKSEAELALSIDSLKDFDDQIRYVLGKHDINDFKKADIVV
KNPSVKPNNKYLKLAKRVETDISLFLIFNKNPIIAVTGTKGKSTLVSLLYQVLKKNYPRAKLGGNIGVSPLSFFDQLDGI
SPLILELSSWQLQSLENFNPILSIITNVYNDHQNYYLNFDDYIIDKSKIFVNQASGITIIQDKAYYKYFSKFESKAKVIL
FSEFNPFVLDQDVFYYNKGEVYFNDNLIGSFSESQVVFMIPKLITFFVSYYLNMDLNHTFKILNKFRGIEHRVEFVKLVQ
NVMFYNDTASTIPDSTVLSVKSLKTNDNSINLIAGGTDKELDFSSFRKIINLVKTWILIKGSATVKIIKILEKSSIQYFV
FDSLRDAVNYAIKISSPGDIVLFSPASASFELFNNEFDRGLQFKNLVNMLG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=461, Percent_Identity=24.5119305856833, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 51232; Mature: 51232

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDEIKNLNFLVMGLGLNGGGVALSRFLLKHGAKLVITDLKSEAELALSIDSLKDFDDQ
CCCCHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHCHHHH
IRYVLGKHDINDFKKADIVVKNPSVKPNNKYLKLAKRVETDISLFLIFNKNPIIAVTGTK
HHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
GKSTLVSLLYQVLKKNYPRAKLGGNIGVSPLSFFDQLDGISPLILELSSWQLQSLENFNP
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEHEECCHHHHHHCCCHH
ILSIITNVYNDHQNYYLNFDDYIIDKSKIFVNQASGITIIQDKAYYKYFSKFESKAKVIL
HHHHHHHHHCCCCCEEEECCCEEEECCEEEEEECCCEEEEECHHHHHHHHHHCCCCEEEE
FSEFNPFVLDQDVFYYNKGEVYFNDNLIGSFSESQVVFMIPKLITFFVSYYLNMDLNHTF
EECCCCEEECCCEEEEECCEEEECCCEECCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
KILNKFRGIEHRVEFVKLVQNVMFYNDTASTIPDSTVLSVKSLKTNDNSINLIAGGTDKE
HHHHHHHCHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCC
LDFSSFRKIINLVKTWILIKGSATVKIIKILEKSSIQYFVFDSLRDAVNYAIKISSPGDI
CCHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHCCCEEEEHHHHHHHCEEEEEECCCCCE
VLFSPASASFELFNNEFDRGLQFKNLVNMLG
EEECCCCCCHHHHCCHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MRLDEIKNLNFLVMGLGLNGGGVALSRFLLKHGAKLVITDLKSEAELALSIDSLKDFDDQ
CCCCHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHCHHHH
IRYVLGKHDINDFKKADIVVKNPSVKPNNKYLKLAKRVETDISLFLIFNKNPIIAVTGTK
HHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
GKSTLVSLLYQVLKKNYPRAKLGGNIGVSPLSFFDQLDGISPLILELSSWQLQSLENFNP
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEHEECCHHHHHHCCCHH
ILSIITNVYNDHQNYYLNFDDYIIDKSKIFVNQASGITIIQDKAYYKYFSKFESKAKVIL
HHHHHHHHHCCCCCEEEECCCEEEECCEEEEEECCCEEEEECHHHHHHHHHHCCCCEEEE
FSEFNPFVLDQDVFYYNKGEVYFNDNLIGSFSESQVVFMIPKLITFFVSYYLNMDLNHTF
EECCCCEEECCCEEEEECCEEEECCCEECCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
KILNKFRGIEHRVEFVKLVQNVMFYNDTASTIPDSTVLSVKSLKTNDNSINLIAGGTDKE
HHHHHHHCHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCC
LDFSSFRKIINLVKTWILIKGSATVKIIKILEKSSIQYFVFDSLRDAVNYAIKISSPGDI
CCHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHCCCEEEEHHHHHHHCEEEEEECCCCCE
VLFSPASASFELFNNEFDRGLQFKNLVNMLG
EEECCCCCCHHHHCCHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA