Click here to switch to the map view.

The map label for this gene is fdoI

Identifier: 51598214

GI number: 51598214

Start: 4692128

End: 4692802

Strand: Direct

Name: fdoI

Synonym: YPTB3929

Alternate gene names: 51598214

Gene position: 4692128-4692802 (Clockwise)

Preceding gene: 51598213

Following gene: 51598215

Centisome position: 98.89

GC content: 50.37

Gene sequence:

>675_bases
ATGAAAAAAGAAAAACCGATCCAGCGCTACAGCGCGCCGGAGCGCATTAACCACTGGATAGTTGCCTTCTGCTTTATATT
GGCGGCTATCAGTGGATTAGGGTTCTTCTTCCCTTCATTTAACTGGCTGATGAATATTTTTGGTACACCACAGATGGCGC
GCATTCTTCACCCCTTTGCCGGGGTGGTGATGTTCGCGGCCTTTTTGATCATGTTTTTCCGCTACTGGAAACATAACCTG
ATCAATAAAGACGACTTGGAGTGGGCCAAAAATATCCATAAAATCGCCATGAATGAGGAAGTGGGTGACACAGGGCGCTA
TAATTTCGGCCAAAAATGCGTATTCTGGGCTGCTATTATCAGTCTGGTGCTACTGTTAGTCAGTGGCGTGGTGATTTGGC
GGCCTTACTTTGCGCCATCCTTCCCTATCCCGCTGATCCGTCTGGCACTACTGGTGCATTCGCTGGCGGCGGTTGGGTTG
ATTATCGTGATTATGGTACATGTGTATGCGGCCCTGTGGGTGAAGGGGACGATTACCGCGATGGTGGAGGGCTGGGTACC
TGCAGCATGGGCTAAAAAGCACCATCCGCGTTGGTATCGTGAAGTCCGCAGTAAAGATGATGCTCGCGCCAACGCTAAAG
GCCAGCCGCAAGAGAAACAGAAAGAGAAACCCTGA

Upstream 100 bases:

>100_bases
AAGAAGACGATGAGACAACGGATCCTACCCCTTCCGAGACGGTAGCAAAGGCACCAGAGCAGACAACCTCTGAGCGCTCA
GACGAAGGGGAAACGCGGAA

Downstream 100 bases:

>100_bases
TGAGTATTCGTATTGTCCCTAAAGACCAATTAGGTAAACAACGCGAAAAAGGTACAACGGCGGGGAATATCCCGCCGTTA
CTTTTCGCAAATTTAAAAAG

Product: formate dehydrogenase-O subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKKEKPIQRYSAPERINHWIVAFCFILAAISGLGFFFPSFNWLMNIFGTPQMARILHPFAGVVMFAAFLIMFFRYWKHNL
INKDDLEWAKNIHKIAMNEEVGDTGRYNFGQKCVFWAAIISLVLLLVSGVVIWRPYFAPSFPIPLIRLALLVHSLAAVGL
IIVIMVHVYAALWVKGTITAMVEGWVPAAWAKKHHPRWYREVRSKDDARANAKGQPQEKQKEKP

Sequences:

>Translated_224_residues
MKKEKPIQRYSAPERINHWIVAFCFILAAISGLGFFFPSFNWLMNIFGTPQMARILHPFAGVVMFAAFLIMFFRYWKHNL
INKDDLEWAKNIHKIAMNEEVGDTGRYNFGQKCVFWAAIISLVLLLVSGVVIWRPYFAPSFPIPLIRLALLVHSLAAVGL
IIVIMVHVYAALWVKGTITAMVEGWVPAAWAKKHHPRWYREVRSKDDARANAKGQPQEKQKEKP
>Mature_224_residues
MKKEKPIQRYSAPERINHWIVAFCFILAAISGLGFFFPSFNWLMNIFGTPQMARILHPFAGVVMFAAFLIMFFRYWKHNL
INKDDLEWAKNIHKIAMNEEVGDTGRYNFGQKCVFWAAIISLVLLLVSGVVIWRPYFAPSFPIPLIRLALLVHSLAAVGL
IIVIMVHVYAALWVKGTITAMVEGWVPAAWAKKHHPRWYREVRSKDDARANAKGQPQEKQKEKP

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=211, Percent_Identity=79.6208530805687, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787750, Length=216, Percent_Identity=48.1481481481481, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 25779; Mature: 25779

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEKPIQRYSAPERINHWIVAFCFILAAISGLGFFFPSFNWLMNIFGTPQMARILHPFA
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVVMFAAFLIMFFRYWKHNLINKDDLEWAKNIHKIAMNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
SLVLLLVSGVVIWRPYFAPSFPIPLIRLALLVHSLAAVGLIIVIMVHVYAALWVKGTITA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVPAAWAKKHHPRWYREVRSKDDARANAKGQPQEKQKEKP
HHHCCCCHHHHHHCCCHHHHHHHCCCHHCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MKKEKPIQRYSAPERINHWIVAFCFILAAISGLGFFFPSFNWLMNIFGTPQMARILHPFA
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVVMFAAFLIMFFRYWKHNLINKDDLEWAKNIHKIAMNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
SLVLLLVSGVVIWRPYFAPSFPIPLIRLALLVHSLAAVGLIIVIMVHVYAALWVKGTITA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVPAAWAKKHHPRWYREVRSKDDARANAKGQPQEKQKEKP
HHHCCCCHHHHHHCCCHHHHHHHCCCHHCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]