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The map label for this gene is fdoH

Identifier: 51598213

GI number: 51598213

Start: 4691160

End: 4692131

Strand: Direct

Name: fdoH

Synonym: YPTB3928

Alternate gene names: 51598213

Gene position: 4691160-4692131 (Clockwise)

Preceding gene: 51598212

Following gene: 51598214

Centisome position: 98.87

GC content: 53.91

Gene sequence:

>972_bases
ATGTCACTGCAAACTCAAGACATTATCCGGCGCTCCGGTACTAATTCCCTTACGCCGCCACCTCAGGTCCGTGATCATCA
GGAACCGGTGGCTAAACTTATCGATGTCACCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACG
ATATCCGCGATGAAGTCGGTCATAACGTCGGGGTGTATGACAACCCTGCCGATTTGACCGCTAAGTCGTGGACAGTGATG
CGTTTCTCTGAAGTTGAAGATGAAGAGAGCGGCAAGCTGGAGTGGCTGATCCGCAAGGATGGCTGTATGCATTGCGCCGA
TCCGGGCTGCCTGAAGGCATGTCCATCGGAAGGGGCGATCATTCAGTACGCCAACGGTATCGTTGATTTCCAATCAGAAC
ATTGTATTGGTTGTGGCTACTGCATCGCAGGTTGTCCGTTCGATGTCCCGCGCATGAATAAAGATGACAATCGGGTGTAT
AAATGCACCTTGTGCGTCGATCGTGTCGGTGTTGGTCAGGAACCTGCTTGTGTGAAAACCTGCCCGACTGGAGCGATTCA
CTTTGGTACCAAAGAGTCGATGAAAGAAGTGGCGGCTGGCCGGGTTGCTGAGCTAAAAACCCGTGGGTTTGATAACGCAG
GGTTATATGACCCTGCGGGCGTCGGCGGTACCCATGTGATGTATGTACTGCATCATGCGGATAAACCCCAGCTTTATCAT
GGCCTGCCGGAGAATCCGACCATCAGTCCGACGGTGACTTTCTGGAAAGGCATCTGGAAACCGTTGGCTGCGGTAGGTTT
CGCGGCGACCTTCGCTGCCAGTATCTTCCATTACGTTGGCGTAGGCCCGAACCGGGTGGAGGAAGAGGAAGAAGACGATG
AGACAACGGATCCTACCCCTTCCGAGACGGTAGCAAAGGCACCAGAGCAGACAACCTCTGAGCGCTCAGACGAAGGGGAA
ACGCGGAAATGA

Upstream 100 bases:

>100_bases
GGCGAATACCCTGACACCGTATGTCGGTGATGCCAATACGCAAACGCCAGAGTTCAAGGCGTTTCTGGTCAATGTGGAAA
AGGTGTAACGGAGAGAATCT

Downstream 100 bases:

>100_bases
AAAAAGAAAAACCGATCCAGCGCTACAGCGCGCCGGAGCGCATTAACCACTGGATAGTTGCCTTCTGCTTTATATTGGCG
GCTATCAGTGGATTAGGGTT

Product: formate dehydrogenase-O, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSLQTQDIIRRSGTNSLTPPPQVRDHQEPVAKLIDVTTCIGCKACQVACSEWNDIRDEVGHNVGVYDNPADLTAKSWTVM
RFSEVEDEESGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVY
KCTLCVDRVGVGQEPACVKTCPTGAIHFGTKESMKEVAAGRVAELKTRGFDNAGLYDPAGVGGTHVMYVLHHADKPQLYH
GLPENPTISPTVTFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRVEEEEEDDETTDPTPSETVAKAPEQTTSERSDEGE
TRK

Sequences:

>Translated_323_residues
MSLQTQDIIRRSGTNSLTPPPQVRDHQEPVAKLIDVTTCIGCKACQVACSEWNDIRDEVGHNVGVYDNPADLTAKSWTVM
RFSEVEDEESGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVY
KCTLCVDRVGVGQEPACVKTCPTGAIHFGTKESMKEVAAGRVAELKTRGFDNAGLYDPAGVGGTHVMYVLHHADKPQLYH
GLPENPTISPTVTFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRVEEEEEDDETTDPTPSETVAKAPEQTTSERSDEGE
TRK
>Mature_322_residues
SLQTQDIIRRSGTNSLTPPPQVRDHQEPVAKLIDVTTCIGCKACQVACSEWNDIRDEVGHNVGVYDNPADLTAKSWTVMR
FSEVEDEESGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYK
CTLCVDRVGVGQEPACVKTCPTGAIHFGTKESMKEVAAGRVAELKTRGFDNAGLYDPAGVGGTHVMYVLHHADKPQLYHG
LPENPTISPTVTFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRVEEEEEDDETTDPTPSETVAKAPEQTTSERSDEGET
RK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=292, Percent_Identity=86.3013698630137, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI1787749, Length=293, Percent_Identity=78.1569965870307, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1789370, Length=185, Percent_Identity=32.4324324324324, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787122, Length=168, Percent_Identity=32.1428571428571, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787872, Length=168, Percent_Identity=32.1428571428571, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=159, Percent_Identity=31.4465408805031, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI226510944, Length=187, Percent_Identity=28.3422459893048, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082179, Length=174, Percent_Identity=28.1609195402299, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787740, Length=110, Percent_Identity=30.9090909090909, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=34.4827586206897, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787963, Length=150, Percent_Identity=25.3333333333333, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788811, Length=176, Percent_Identity=23.2954545454545, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082180, Length=164, Percent_Identity=27.4390243902439, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 35241; Mature: 35110

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQTQDIIRRSGTNSLTPPPQVRDHQEPVAKLIDVTTCIGCKACQVACSEWNDIRDEVG
CCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
HNVGVYDNPADLTAKSWTVMRFSEVEDEESGKLEWLIRKDGCMHCADPGCLKACPSEGAI
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCE
IQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKCTLCVDRVGVGQEPACVKT
EEECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEE
CPTGAIHFGTKESMKEVAAGRVAELKTRGFDNAGLYDPAGVGGTHVMYVLHHADKPQLYH
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHC
GLPENPTISPTVTFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRVEEEEEDDETTDPTP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SETVAKAPEQTTSERSDEGETRK
HHHHHCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLQTQDIIRRSGTNSLTPPPQVRDHQEPVAKLIDVTTCIGCKACQVACSEWNDIRDEVG
CCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
HNVGVYDNPADLTAKSWTVMRFSEVEDEESGKLEWLIRKDGCMHCADPGCLKACPSEGAI
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCE
IQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKCTLCVDRVGVGQEPACVKT
EEECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEE
CPTGAIHFGTKESMKEVAAGRVAELKTRGFDNAGLYDPAGVGGTHVMYVLHHADKPQLYH
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHC
GLPENPTISPTVTFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRVEEEEEDDETTDPTP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SETVAKAPEQTTSERSDEGETRK
HHHHHCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]