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The map label for this gene is cysD

Identifier: 51595115

GI number: 51595115

Start: 921656

End: 922564

Strand: Direct

Name: cysD

Synonym: YPTB0765

Alternate gene names: 51595115

Gene position: 921656-922564 (Clockwise)

Preceding gene: 51595114

Following gene: 51595116

Centisome position: 19.43

GC content: 49.39

Gene sequence:

>909_bases
ATGGACGAAAAACGACTCACTCATTTACGGCAATTGGAGGCGGAGAGTATCCATATCATCCGTGAAGTGGCCGCTGAATT
CGGTAACCCGGTGATGCTCTATTCTATCGGCAAGGACTCCTCTGTGATGCTGCATTTGGCGCGCAAGGCATTCTTCCCCG
GTCATTTACCGTTTCCCCTATTGCATGTGGATACGGGATGGAAATTTCGTGAGATGTATGAATTTCGCGATCACACGGTC
AAAGAGTTTGGCTGTGAGTTGTTGGTTCATCGCAATCCGGAAGGGGTGGCGATGGGGATTAACCCCTTTGTCCACGGTAG
TGCCAAACATACCGATATCATGAAAACTGAAGGTTTGAAGCAGGCGTTGAACAAATACGGTTTTGATGCCGCTTTCGGTG
GTGCGCGGCGTGATGAAGAAAAATCCCGTGCCAAAGAGCGTATTTATTCTTTCCGTGACCGTTTCCACCGTTGGGATCCT
AAAAATCAACGTCCTGAGCTGTGGCACAACTACAACGGCCAGATTAACAAAGGCGAAAGCATCCGCGTATTCCCTCTGTC
CAACTGGACCGAGTTGGATATCTGGCAATATATTTTCCTCGAGAAAATTGAGATTGTTCCGTTGTATCTGGCGAAGCCAC
GCCCGGTAGTTGAACGCGATGGCATGTTGTTAATGGTTGATGATGATCGTATCGATTTGCAGCCAGGTGAAGTCATTGTT
CAGAAGAAAGTGCGTTTCCGGACGTTAGGCTGCTGGCCGCTGACAGGGGCTGTCGAGTCTGAGGCTGAAACCTTACCGGC
GATTATCGAAGAGATGTTGATCTCTACCACCAGTGAACGCCAGGGTCGAATGATCGACCGCGATCAATCCGGCTCCATGG
AGCTTAAAAAGCGTCAGGGATATTTCTGA

Upstream 100 bases:

>100_bases
GTGGTGAATTTACATCACCAAATTGCCTGGTTTGGGCAACAACCGCAGACTGAATCGGCGATCAGCCCATCAGTCGTGAA
TTTGGCATAAAGGATCTGTT

Downstream 100 bases:

>100_bases
GGAGCGGCCCGATGAGCACGATATTGCAAAACAAGCAGACATTACCAAGTGAACCTATTGCTAAAAATCAGTCAGCATTA
GAAAGTAACTCAATGATTTT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGHLPFPLLHVDTGWKFREMYEFRDHTV
KEFGCELLVHRNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWHNYNGQINKGESIRVFPLSNWTELDIWQYIFLEKIEIVPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIV
QKKVRFRTLGCWPLTGAVESEAETLPAIIEEMLISTTSERQGRMIDRDQSGSMELKKRQGYF

Sequences:

>Translated_302_residues
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGHLPFPLLHVDTGWKFREMYEFRDHTV
KEFGCELLVHRNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWHNYNGQINKGESIRVFPLSNWTELDIWQYIFLEKIEIVPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIV
QKKVRFRTLGCWPLTGAVESEAETLPAIIEEMLISTTSERQGRMIDRDQSGSMELKKRQGYF
>Mature_302_residues
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGHLPFPLLHVDTGWKFREMYEFRDHTV
KEFGCELLVHRNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWHNYNGQINKGESIRVFPLSNWTELDIWQYIFLEKIEIVPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIV
QKKVRFRTLGCWPLTGAVESEAETLPAIIEEMLISTTSERQGRMIDRDQSGSMELKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=90.0662251655629, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35188; Mature: 35188

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGHLPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCE
LHVDTGWKFREMYEFRDHTVKEFGCELLVHRNPEGVAMGINPFVHGSAKHTDIMKTEGLK
EEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWHNYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCE
IRVFPLSNWTELDIWQYIFLEKIEIVPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIV
EEEEECCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEECCCEECCCCHHHE
QKKVRFRTLGCWPLTGAVESEAETLPAIIEEMLISTTSERQGRMIDRDQSGSMELKKRQG
ECCCEEEEECCEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHCCC
YF
CC
>Mature Secondary Structure
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGHLPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCE
LHVDTGWKFREMYEFRDHTVKEFGCELLVHRNPEGVAMGINPFVHGSAKHTDIMKTEGLK
EEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWHNYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCE
IRVFPLSNWTELDIWQYIFLEKIEIVPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIV
EEEEECCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEECCCEECCCCHHHE
QKKVRFRTLGCWPLTGAVESEAETLPAIIEEMLISTTSERQGRMIDRDQSGSMELKKRQG
ECCCEEEEECCEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHCCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA