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The map label for this gene is cysG

Identifier: 51595114

GI number: 51595114

Start: 920227

End: 921645

Strand: Direct

Name: cysG

Synonym: YPTB0764

Alternate gene names: 51595114

Gene position: 920227-921645 (Clockwise)

Preceding gene: 51595111

Following gene: 51595115

Centisome position: 19.39

GC content: 55.81

Gene sequence:

>1419_bases
GTGGACTATCTACCTCTATTTGCCGACTTGAAACAACGCCCGGTATTGATCGTTGGCGGCGGCGAAGTTGCTGCGCGAAA
AATTGAACTACTGCACCGCGCAGGTGCGCAGGTATGGGTAGTGGCGCAAACCCTCTCATCTGAACTCGAACAGCAGTATC
AGGATGGCCGTATCCATTGGCTGGCACAGGATTTTCTGCCTGAACAGTTGGACAACGTGTTTCTGGTGATTGCCGCAACT
AACGATACCGTACTGAATGCCGCTGTTTTCGCGGCGGCAGATCAACGGTGTATTTTGGCGAATGTGGTGGATGACCAACC
CCTTTGTTCGTTTATTTTCCCTTCGATTGTTGATCGTTCACCGCTGGTGGTCGCGATCTCGTCTTCCGGCCAGGCACCGG
TGCTGGCGCGGATACTGCGTGAGAAGTTAGAAGCCTTACTGCCCACTCGTCTCAGTGATATGGCCGCTATAGCTGGGCGC
TGGCGTGGGCGGGTTAAGCAGCATATGGCCTCTATGGGGGAGCGCCGCCGCTTTTGGGAACATGCATTCAGCGGGCGCTT
TGCCAGCCTTATCAGCCGTGGTCAGTTAACCGAGGCTGAAAATGAATTACAACTGTCGCTAGAGGGCCAACACCGTGCAC
TCGGCGAAGTGGCTTTGGTCGGTGCAGGTCCTGGCGATGCAGGGTTATTAACGTTACGTGGTTTGCAAGTGATGCAGCAG
GCAGATGTGGTGCTGTATGACCATTTAGTCAGTCCGGAAGTCTTGGATTTGGTGCGTCGCGATGCTGAACGCATCTGTGT
GGGGAAACGGGCGGGGGCGCATTCGGTTACCCAAGAGGCAACCAATCAGTTGTTGGTCACTCTGGCACAGCAAGGAAAAC
GGGTCGTACGGCTAAAAGGTGGCGATCCCTTTATTTTTGGTCGGGGGGGGGAAGAGTTACAAGTTGTGGCGCAAGCGGGG
ATCCCGTTTCAGGTGGTACCGGGGGTGACGGCTGCTGCGGGGGCGACAGCCTATGCCGGTATTCCCCTGACACACCGCGA
TCATGCGCAAAGCGTGACATTTATTACCGGGCATTGCCGTCCTGATGGCGATGATCTTGATTGGCAGGCGCTGGCCCGTG
GTCGTCAGACGCTGGCGATCTACATGGGAACAGTGAAAGCGGCGGCAATCAGCCAACAGTTGATCGCCCATGGCCGGTCC
AGTACCACGCCGGTGGCAGTGATTGGCCGTGGAACCCGTGTGGACCAGCAGGTGCTGATCGGTACGTTGGCACAACTTGA
ATCACTCGCACAGCAGGCACCGACACCAGCACTACTGGTGATTGGTGAAGTGGTGAATTTACATCACCAAATTGCCTGGT
TTGGGCAACAACCGCAGACTGAATCGGCGATCAGCCCATCAGTCGTGAATTTGGCATAA

Upstream 100 bases:

>100_bases
ATATATCATTATGGAACTTGTAATTACTTTTCGTCATTTCATAAGGTCATGGCCCCCCCCTATAGTCACCCTACGCTTTA
TCATGTAATCGAAGGCTGTT

Downstream 100 bases:

>100_bases
AGGATCTGTTATGGACGAAAAACGACTCACTCATTTACGGCAATTGGAGGCGGAGAGTATCCATATCATCCGTGAAGTGG
CCGCTGAATTCGGTAACCCG

Product: siroheme synthase, catalyses four separate reactions required for the transformation of uroporphyrinogen III into siroheme

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHWLAQDFLPEQLDNVFLVIAAT
NDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRSPLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGR
WRGRVKQHMASMGERRRFWEHAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAG
IPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRS
STTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAISPSVVNLA

Sequences:

>Translated_472_residues
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHWLAQDFLPEQLDNVFLVIAAT
NDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRSPLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGR
WRGRVKQHMASMGERRRFWEHAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAG
IPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRS
STTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAISPSVVNLA
>Mature_472_residues
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHWLAQDFLPEQLDNVFLVIAAT
NDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRSPLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGR
WRGRVKQHMASMGERRRFWEHAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAG
IPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRS
STTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAISPSVVNLA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=460, Percent_Identity=56.304347826087, Blast_Score=478, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322922, Length=255, Percent_Identity=34.5098039215686, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50875; Mature: 50875

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHW
CCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEE
LAQDFLPEQLDNVFLVIAATNDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRS
EHHHHCHHHCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC
PLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGRWRGRVKQHMASMGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
HHHCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKG
CCCCEEHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GDPFIFGRGGEELQVVAQAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCR
CCEEEECCCCCEEEEEEECCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEEECC
PDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRSSTTPVAVIGRGTRVDQQVLI
CCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
GTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAISPSVVNLA
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHW
CCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEE
LAQDFLPEQLDNVFLVIAATNDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRS
EHHHHCHHHCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC
PLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGRWRGRVKQHMASMGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
HHHCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKG
CCCCEEHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GDPFIFGRGGEELQVVAQAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCR
CCEEEECCCCCEEEEEEECCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEEECC
PDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRSSTTPVAVIGRGTRVDQQVLI
CCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
GTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAISPSVVNLA
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA