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The map label for this gene is poxA

Identifier: 51594766

GI number: 51594766

Start: 492257

End: 493234

Strand: Direct

Name: poxA

Synonym: YPTB0414

Alternate gene names: 51594766

Gene position: 492257-493234 (Clockwise)

Preceding gene: 51594761

Following gene: 51594770

Centisome position: 10.37

GC content: 49.39

Gene sequence:

>978_bases
ATGAGCGACACGGCAAGCTGGCAGCCAAGTGCACCTATCGCCAATTTGTTGAAGCGCGCAGCAATAATGGCAGAGATTCG
GCGCTTTTTCGCCGATCGGGGCGTATTAGAAGTAGAAACGCCAACCATGAGCCAAGCGACAGTGACGGACATTCATTTAG
TTCCATTCGAAACCCGGTTTGTTGGCCCGGGCGCTGCCGATGGTTTGACGCTGTACATGATGACCAGCCCGGAATATCAC
ATGAAGCGGTTGTTAGCGGCGGGCAGTGGCCCTATCTATCAACTGGGCCGCAGTTTCCGCAATGAAGAGGCTGGCCGCTA
TCATAATCCTGAATTCACCATGCTGGAATGGTACCGCCCTCATTATGATATGTATCGTTTAATGAATGAGGTGGATGACC
TGTTGCAGCAAATTTTGGATTGCAACAGCGCAGAGACCTTATCCTATCAGCAAGCGTTTTTGCGCCATTTAAATATTGAT
CCGTTGTCTGCTGAAAAAGCACAATTGCGTGAAGTGGCGGCAAAACTGGATTTAAGCAATATTGCGGATACCGAAGAAGA
TCGCGATACGTTGCTGCAATTGTTGTTTACTGTGGGTGTCGAACCTTATATTGGCCGTGATAAACCGGCATTTATTTATC
ATTTCCCGGCATCGCAGGCATCTCTGGCTGAAATCAGTACCGAAGACCACCGTGTGGCGGAACGCTTTGAGGTGTACTTC
AAGGGGATAGAACTAGCGAATGGTTTCCGAGAGCTGACTGACGGTGATGAGCAACTGCAACGCTTTGAGCAGGATAACCG
TAACCGTGCGAAACGGGGCTTACCACAGAACCCAATCGATATGAACCTTATCGCAGCACTAAAGCAGGGTTTACCCGATT
GTTCAGGTGTGGCTTTAGGGGTTGATCGCTTGGTGATGCTGGCGTTAAATGCGGAAAGGTTGAGTGATGTTATTGCCTTC
CCCGTCAATATCGCCTAA

Upstream 100 bases:

>100_bases
CTAGAACCAGACCGAGAATTCGGCGGATGAGTGACAGATTTGTCTGGGGCGCGGGATACAGGTAGACTGCATCCCCTGTT
TGATTTCGGAGTTAACCACC

Downstream 100 bases:

>100_bases
TTCCCCTTCGTCCTTGGCGCCGCAGCGGTGTTAGCGGCGTGCACTCACCCGAATCACTTACTGATGTAAGCTCATCGGGA
TTCGTTTACTTGCTGCCTTG

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSDTASWQPSAPIANLLKRAAIMAEIRRFFADRGVLEVETPTMSQATVTDIHLVPFETRFVGPGAADGLTLYMMTSPEYH
MKRLLAAGSGPIYQLGRSFRNEEAGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQILDCNSAETLSYQQAFLRHLNID
PLSAEKAQLREVAAKLDLSNIADTEEDRDTLLQLLFTVGVEPYIGRDKPAFIYHFPASQASLAEISTEDHRVAERFEVYF
KGIELANGFRELTDGDEQLQRFEQDNRNRAKRGLPQNPIDMNLIAALKQGLPDCSGVALGVDRLVMLALNAERLSDVIAF
PVNIA

Sequences:

>Translated_325_residues
MSDTASWQPSAPIANLLKRAAIMAEIRRFFADRGVLEVETPTMSQATVTDIHLVPFETRFVGPGAADGLTLYMMTSPEYH
MKRLLAAGSGPIYQLGRSFRNEEAGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQILDCNSAETLSYQQAFLRHLNID
PLSAEKAQLREVAAKLDLSNIADTEEDRDTLLQLLFTVGVEPYIGRDKPAFIYHFPASQASLAEISTEDHRVAERFEVYF
KGIELANGFRELTDGDEQLQRFEQDNRNRAKRGLPQNPIDMNLIAALKQGLPDCSGVALGVDRLVMLALNAERLSDVIAF
PVNIA
>Mature_324_residues
SDTASWQPSAPIANLLKRAAIMAEIRRFFADRGVLEVETPTMSQATVTDIHLVPFETRFVGPGAADGLTLYMMTSPEYHM
KRLLAAGSGPIYQLGRSFRNEEAGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQILDCNSAETLSYQQAFLRHLNIDP
LSAEKAQLREVAAKLDLSNIADTEEDRDTLLQLLFTVGVEPYIGRDKPAFIYHFPASQASLAEISTEDHRVAERFEVYFK
GIELANGFRELTDGDEQLQRFEQDNRNRAKRGLPQNPIDMNLIAALKQGLPDCSGVALGVDRLVMLALNAERLSDVIAFP
VNIA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=338, Percent_Identity=28.698224852071, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI5031815, Length=338, Percent_Identity=28.698224852071, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=84, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1790571, Length=322, Percent_Identity=30.4347826086957, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1789256, Length=329, Percent_Identity=30.3951367781155, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=335, Percent_Identity=28.3582089552239, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=335, Percent_Identity=28.3582089552239, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=341, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324256, Length=349, Percent_Identity=27.2206303724928, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640851, Length=336, Percent_Identity=28.2738095238095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24640849, Length=336, Percent_Identity=28.2738095238095, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJEA_YERP3 (A7FMZ1)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402619.1
- ProteinModelPortal:   A7FMZ1
- SMR:   A7FMZ1
- STRING:   A7FMZ1
- GeneID:   5386806
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3665
- eggNOG:   COG2269
- HOGENOM:   HBG631383
- OMA:   MPEASGI
- ProtClustDB:   PRK09350
- BioCyc:   YPSE349747:YPSIP31758_3665-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00174
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149
- PANTHER:   PTHR22594
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00462

Pfam domain/function: PF00152 tRNA-synt_2

EC number: 6.1.1.6

Molecular weight: Translated: 36700; Mature: 36569

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTASWQPSAPIANLLKRAAIMAEIRRFFADRGVLEVETPTMSQATVTDIHLVPFETRF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCEEE
VGPGAADGLTLYMMTSPEYHMKRLLAAGSGPIYQLGRSFRNEEAGRYHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEHHCC
HYDMYRLMNEVDDLLQQILDCNSAETLSYQQAFLRHLNIDPLSAEKAQLREVAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHH
IADTEEDRDTLLQLLFTVGVEPYIGRDKPAFIYHFPASQASLAEISTEDHRVAERFEVYF
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHHH
KGIELANGFRELTDGDEQLQRFEQDNRNRAKRGLPQNPIDMNLIAALKQGLPDCSGVALG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
VDRLVMLALNAERLSDVIAFPVNIA
HHHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDTASWQPSAPIANLLKRAAIMAEIRRFFADRGVLEVETPTMSQATVTDIHLVPFETRF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCEEE
VGPGAADGLTLYMMTSPEYHMKRLLAAGSGPIYQLGRSFRNEEAGRYHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEHHCC
HYDMYRLMNEVDDLLQQILDCNSAETLSYQQAFLRHLNIDPLSAEKAQLREVAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHH
IADTEEDRDTLLQLLFTVGVEPYIGRDKPAFIYHFPASQASLAEISTEDHRVAERFEVYF
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHHH
KGIELANGFRELTDGDEQLQRFEQDNRNRAKRGLPQNPIDMNLIAALKQGLPDCSGVALG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
VDRLVMLALNAERLSDVIAFPVNIA
HHHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA