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The map label for this gene is cbiX [H]

Identifier: 50841906

GI number: 50841906

Start: 468133

End: 469401

Strand: Direct

Name: cbiX [H]

Synonym: PPA0423

Alternate gene names: 50841906

Gene position: 468133-469401 (Clockwise)

Preceding gene: 50841905

Following gene: 50841907

Centisome position: 18.28

GC content: 64.62

Gene sequence:

>1269_bases
ATGACCGATCCCCTCACCAGCGGAACTCCGCTGGTCATCGCTGCCCACGGCACCCGCGACGAGGCAGGTGTGGCCGAGTG
CCGTGCTTTGGCTGAGCGCGTTGCTCGCAAGCTGCCTGGTATCCCGGTGGAACTGGGGTTCGTCGAGCTCGCCGAACCCG
GCATCCCCGAGGCCGTCGGTGCCGCCGTCGCCCAGGCCCCAGACAATCCCGAGGGGGCCGGCGGCGACGAGGAGGCAGCC
GCTGTCGTCGTGCCACTGATGTTCAACACTGGCGGTCACGTCCGCGAGGATATTCCGGAGGCAATTGACGAGGGCCGCGG
GGACGCCCGGGTGATGTATTCCGCCCCGCTGTTGCCGGACGTTCGGATGCGCAAGGCCCTCAACCATCGTCTCACCGAGA
CCCTTGCCGCTGGTGAAGGATGCGAGGAGTGGAGGCCTCGGGACACGTCGGCAGTGCTCGTGGGGCGTGGCGCCCTAGTG
CCTGACGCCAACGCCTCTCACTACACCCTGACGCGAATGTTCTGGGAGGAAAACGACCTGTCGCGGGTCGAGCCTGCTTT
CATTCAGGTGACTCGTCCGTCAGTCCCGGAAGCCTTGTCTGCCTTAGCCGCGCAGGGGGCCAACCAGATCGTCGTGCAGG
GCAATTTCTTGTTCCCAGGCCTGCTACATGTGTGGATGACCGAACAGGTGGAGGCCTGGCAAGCTAGCCACCCCGGAGTC
GAGATCCGTATTGCTCCGGTAATTGGTGACTGTGACGAGGTCGCAGATGTCGTCGTCGATCGTTACCGCGAGCCTCTCGA
CGAGGTCGGTCTGGGGGAAGGAGCTCCCGTTTATATGTCGGGCCTGAGGTTGCAGGGGCGCAAGGTGCTGGTCGTCGGTG
CGGGGCACGTGGCGGAGCGTCGCGTGGTGCGGTTGTTGGAGGCGGGCGCCGACGTGCATGTCGTGGCCCCAAATTCTGGC
ATCCAGCTTACCCGCATGTTCCGTAAAGGATTGGTGAACCTGGAGCGTCGGGCTTTCCAGACTGAGGATCTTGACGGAGT
CTGGTTCGTCCAGGCACTCACGAACGATCCAGAAGTCAATGCCCTGGTGGCCGAAGAGGCCGACAGGCGAGGCATCTTCT
GCGTGCGTGGCGATAACAGCCGCAAGGGCAGTGCTTTCACCCCGGCCACCCAGCAGGCTGGCGGCATGACAGTCAGCGTC
GTCGGTGACCGCACCCCACGTCGTTCTGCCAGGCTGCGGGATGAGGTGCTGCGAGCCTTGCAAGGCTGA

Upstream 100 bases:

>100_bases
ACAAGGACTTGCCCAAGGCCGACGAAGAGACTTATCTCTCCAAGCCTCCGGTTCACTCCACTCGCATGTCTGAGCCCATA
AACGACACAAAGGATTCGAA

Downstream 100 bases:

>100_bases
TCAATGGGGTCATTCATGACACGGCGTGGGATCGCTCGACCATGACGGGCGCGTCTGGTGGTGCATATCATCGTCGGGTG
ATTTCCCACGACCTGCATGC

Product: cobalamin synthesis protein, fusion CysG+CbiX

Products: NA

Alternate protein names: CbiXL [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTDPLTSGTPLVIAAHGTRDEAGVAECRALAERVARKLPGIPVELGFVELAEPGIPEAVGAAVAQAPDNPEGAGGDEEAA
AVVVPLMFNTGGHVREDIPEAIDEGRGDARVMYSAPLLPDVRMRKALNHRLTETLAAGEGCEEWRPRDTSAVLVGRGALV
PDANASHYTLTRMFWEENDLSRVEPAFIQVTRPSVPEALSALAAQGANQIVVQGNFLFPGLLHVWMTEQVEAWQASHPGV
EIRIAPVIGDCDEVADVVVDRYREPLDEVGLGEGAPVYMSGLRLQGRKVLVVGAGHVAERRVVRLLEAGADVHVVAPNSG
IQLTRMFRKGLVNLERRAFQTEDLDGVWFVQALTNDPEVNALVAEEADRRGIFCVRGDNSRKGSAFTPATQQAGGMTVSV
VGDRTPRRSARLRDEVLRALQG

Sequences:

>Translated_422_residues
MTDPLTSGTPLVIAAHGTRDEAGVAECRALAERVARKLPGIPVELGFVELAEPGIPEAVGAAVAQAPDNPEGAGGDEEAA
AVVVPLMFNTGGHVREDIPEAIDEGRGDARVMYSAPLLPDVRMRKALNHRLTETLAAGEGCEEWRPRDTSAVLVGRGALV
PDANASHYTLTRMFWEENDLSRVEPAFIQVTRPSVPEALSALAAQGANQIVVQGNFLFPGLLHVWMTEQVEAWQASHPGV
EIRIAPVIGDCDEVADVVVDRYREPLDEVGLGEGAPVYMSGLRLQGRKVLVVGAGHVAERRVVRLLEAGADVHVVAPNSG
IQLTRMFRKGLVNLERRAFQTEDLDGVWFVQALTNDPEVNALVAEEADRRGIFCVRGDNSRKGSAFTPATQQAGGMTVSV
VGDRTPRRSARLRDEVLRALQG
>Mature_421_residues
TDPLTSGTPLVIAAHGTRDEAGVAECRALAERVARKLPGIPVELGFVELAEPGIPEAVGAAVAQAPDNPEGAGGDEEAAA
VVVPLMFNTGGHVREDIPEAIDEGRGDARVMYSAPLLPDVRMRKALNHRLTETLAAGEGCEEWRPRDTSAVLVGRGALVP
DANASHYTLTRMFWEENDLSRVEPAFIQVTRPSVPEALSALAAQGANQIVVQGNFLFPGLLHVWMTEQVEAWQASHPGVE
IRIAPVIGDCDEVADVVVDRYREPLDEVGLGEGAPVYMSGLRLQGRKVLVVGAGHVAERRVVRLLEAGADVHVVAPNSGI
QLTRMFRKGLVNLERRAFQTEDLDGVWFVQALTNDPEVNALVAEEADRRGIFCVRGDNSRKGSAFTPATQQAGGMTVSVV
GDRTPRRSARLRDEVLRALQG

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG2138

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiX family. CbiXL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=145, Percent_Identity=33.7931034482759, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002762 [H]

Pfam domain/function: PF01903 CbiX [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 45334; Mature: 45203

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPLTSGTPLVIAAHGTRDEAGVAECRALAERVARKLPGIPVELGFVELAEPGIPEAVG
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEECEEEECCCCCCHHHH
AAVAQAPDNPEGAGGDEEAAAVVVPLMFNTGGHVREDIPEAIDEGRGDARVMYSAPLLPD
HHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCH
VRMRKALNHRLTETLAAGEGCEEWRPRDTSAVLVGRGALVPDANASHYTLTRMFWEENDL
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCC
SRVEPAFIQVTRPSVPEALSALAAQGANQIVVQGNFLFPGLLHVWMTEQVEAWQASHPGV
CCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCE
EIRIAPVIGDCDEVADVVVDRYREPLDEVGLGEGAPVYMSGLRLQGRKVLVVGAGHVAER
EEEEEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEECEEECCCEEEEEECCHHHHH
RVVRLLEAGADVHVVAPNSGIQLTRMFRKGLVNLERRAFQTEDLDGVWFVQALTNDPEVN
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCE
ALVAEEADRRGIFCVRGDNSRKGSAFTPATQQAGGMTVSVVGDRTPRRSARLRDEVLRAL
EEEECCCCCCCEEEEECCCCCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
QG
CC
>Mature Secondary Structure 
TDPLTSGTPLVIAAHGTRDEAGVAECRALAERVARKLPGIPVELGFVELAEPGIPEAVG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEECEEEECCCCCCHHHH
AAVAQAPDNPEGAGGDEEAAAVVVPLMFNTGGHVREDIPEAIDEGRGDARVMYSAPLLPD
HHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCH
VRMRKALNHRLTETLAAGEGCEEWRPRDTSAVLVGRGALVPDANASHYTLTRMFWEENDL
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCC
SRVEPAFIQVTRPSVPEALSALAAQGANQIVVQGNFLFPGLLHVWMTEQVEAWQASHPGV
CCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCE
EIRIAPVIGDCDEVADVVVDRYREPLDEVGLGEGAPVYMSGLRLQGRKVLVVGAGHVAER
EEEEEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEECEEECCCEEEEEECCHHHHH
RVVRLLEAGADVHVVAPNSGIQLTRMFRKGLVNLERRAFQTEDLDGVWFVQALTNDPEVN
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCE
ALVAEEADRRGIFCVRGDNSRKGSAFTPATQQAGGMTVSVVGDRTPRRSARLRDEVLRAL
EEEECCCCCCCEEEEECCCCCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
QG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9742225; 12917443 [H]