Click here to switch to the map view.

The map label for this gene is cbiF [H]

Identifier: 50841904

GI number: 50841904

Start: 464598

End: 465569

Strand: Direct

Name: cbiF [H]

Synonym: PPA0421

Alternate gene names: 50841904

Gene position: 464598-465569 (Clockwise)

Preceding gene: 50841903

Following gene: 50841905

Centisome position: 18.15

GC content: 63.27

Gene sequence:

>972_bases
GTGAGCGAACCTCAGGCCGCACGGCCCACCCAGCATGTGTCTGAGAAGGCTCTGGAGGATTTAGACCACAACGTCATCGA
GCCGCCGCCGGTGCCCCGTCGTGACATCCCCGAAGGAGCCGGCAAGGTCGTTTTCGTTGGAGCCGGACCGGGTGCCCCAG
ACTTGGTGAGTGTGCGCGGCGCTCGGGTCATTGAAACCGCCGACATCATCATCTGGGCCTCCAGCCTGGTACATCCCGAC
ATGGTTGCCCGTCACAAGGAGGGCGCTGAGCTCATCGACTCCGCCTCTATCCCCTTGGAGGACCTTGAACCTCTCTACAC
CCGCGCCCGCGACGAGGGCCTGATGGTGGCCAGGGTCCACACCGGTGACCCGTCCATCTACGGGGCTACCGCAGAACAGC
GTGACCTGTGTCGTCGTATTGGTATTGACTTCGAGACCATTCCTGGCATCTCTGCTTTCTCGGCTGCCGCTGCACGGATG
GACGTCGAGATCACTGTCCCGGAAGTCTCTCAGTCACTCATCCTCACCCGGCTCGAGGGCGGGCGCACACCCATGCCCGA
CGGTGAGACCGTTGCCTCCTTCGCCCGTCATGGGGCGACTATGGCAATCTACCTGTCAGCGGCCCGTAACAAGGCGCTTC
AGGAGGCCTTACTGGAGGGCGGATATCTGCCGGAGACCCCCTGCATCATCGGGTATGCGGTGACCTGGCCCGAGGAGATG
ATGTTCCGCTGCGACCTGGCTCACCTGTCGGAGACGATGCGTAACCACAAGCTGTGGAAGCATGCGGTCGTCCTCGTGGG
CCCGGCGCTGGCAGAGGGGCCGATCTCCACCCGCAGCCACCTCTATCATCCCGGTTTTCGTCACGAGTATCGCGCAGCCG
ACGCTCAGGCCCACGCTGACCTCAAGGCTCACGGTACCTGCGGCGTCTACCACCGGAACTCCACGAACCCCGACACGAAG
GGCAATTCATGA

Upstream 100 bases:

>100_bases
AGCAGATCATCACCCTTGACGAAGTGGCTGACGCGCAGACTCTGCCGTATTTCTCGACCGTCTTGACCACTCCGGAGCGC
ACCACCACAGGAGGGAAACT

Downstream 100 bases:

>100_bases
TTCGCGTCCACGGATATCTCGGCACTATTAGCGACGCCCTGCGCGAGGAAGTTACCCAGGCTGATCTCGTCGTCGGTGGT
CAGCGTCACCTTGATGCCCT

Product: tetrapyrrole methylase, putative precorrin-3 methylase, CbiF

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSEPQAARPTQHVSEKALEDLDHNVIEPPPVPRRDIPEGAGKVVFVGAGPGAPDLVSVRGARVIETADIIIWASSLVHPD
MVARHKEGAELIDSASIPLEDLEPLYTRARDEGLMVARVHTGDPSIYGATAEQRDLCRRIGIDFETIPGISAFSAAAARM
DVEITVPEVSQSLILTRLEGGRTPMPDGETVASFARHGATMAIYLSAARNKALQEALLEGGYLPETPCIIGYAVTWPEEM
MFRCDLAHLSETMRNHKLWKHAVVLVGPALAEGPISTRSHLYHPGFRHEYRAADAQAHADLKAHGTCGVYHRNSTNPDTK
GNS

Sequences:

>Translated_323_residues
MSEPQAARPTQHVSEKALEDLDHNVIEPPPVPRRDIPEGAGKVVFVGAGPGAPDLVSVRGARVIETADIIIWASSLVHPD
MVARHKEGAELIDSASIPLEDLEPLYTRARDEGLMVARVHTGDPSIYGATAEQRDLCRRIGIDFETIPGISAFSAAAARM
DVEITVPEVSQSLILTRLEGGRTPMPDGETVASFARHGATMAIYLSAARNKALQEALLEGGYLPETPCIIGYAVTWPEEM
MFRCDLAHLSETMRNHKLWKHAVVLVGPALAEGPISTRSHLYHPGFRHEYRAADAQAHADLKAHGTCGVYHRNSTNPDTK
GNS
>Mature_322_residues
SEPQAARPTQHVSEKALEDLDHNVIEPPPVPRRDIPEGAGKVVFVGAGPGAPDLVSVRGARVIETADIIIWASSLVHPDM
VARHKEGAELIDSASIPLEDLEPLYTRARDEGLMVARVHTGDPSIYGATAEQRDLCRRIGIDFETIPGISAFSAAAARMD
VEITVPEVSQSLILTRLEGGRTPMPDGETVASFARHGATMAIYLSAARNKALQEALLEGGYLPETPCIIGYAVTWPEEMM
FRCDLAHLSETMRNHKLWKHAVVLVGPALAEGPISTRSHLYHPGFRHEYRAADAQAHADLKAHGTCGVYHRNSTNPDTKG
NS

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=238, Percent_Identity=27.3109243697479, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322922, Length=306, Percent_Identity=22.5490196078431, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 35021; Mature: 34890

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPQAARPTQHVSEKALEDLDHNVIEPPPVPRRDIPEGAGKVVFVGAGPGAPDLVSVRG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCC
ARVIETADIIIWASSLVHPDMVARHKEGAELIDSASIPLEDLEPLYTRARDEGLMVARVH
CEEEEHHHEEEEHHHHCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEE
TGDPSIYGATAEQRDLCRRIGIDFETIPGISAFSAAAARMDVEITVPEVSQSLILTRLEG
CCCCCEEECCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHCC
GRTPMPDGETVASFARHGATMAIYLSAARNKALQEALLEGGYLPETPCIIGYAVTWPEEM
CCCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHH
MFRCDLAHLSETMRNHKLWKHAVVLVGPALAEGPISTRSHLYHPGFRHEYRAADAQAHAD
HHHHHHHHHHHHHHHHHHHHHHEEEECCHHCCCCCCCCCCCCCCCCCCHHHHCCHHHCCC
LKAHGTCGVYHRNSTNPDTKGNS
EECCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SEPQAARPTQHVSEKALEDLDHNVIEPPPVPRRDIPEGAGKVVFVGAGPGAPDLVSVRG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCC
ARVIETADIIIWASSLVHPDMVARHKEGAELIDSASIPLEDLEPLYTRARDEGLMVARVH
CEEEEHHHEEEEHHHHCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEE
TGDPSIYGATAEQRDLCRRIGIDFETIPGISAFSAAAARMDVEITVPEVSQSLILTRLEG
CCCCCEEECCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHCC
GRTPMPDGETVASFARHGATMAIYLSAARNKALQEALLEGGYLPETPCIIGYAVTWPEEM
CCCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHH
MFRCDLAHLSETMRNHKLWKHAVVLVGPALAEGPISTRSHLYHPGFRHEYRAADAQAHAD
HHHHHHHHHHHHHHHHHHHHHHEEEECCHHCCCCCCCCCCCCCCCCCCHHHHCCHHHCCC
LKAHGTCGVYHRNSTNPDTKGNS
EECCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]