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The map label for this gene is cobD [H]

Identifier: 50841900

GI number: 50841900

Start: 461019

End: 462047

Strand: Direct

Name: cobD [H]

Synonym: PPA0418

Alternate gene names: 50841900

Gene position: 461019-462047 (Clockwise)

Preceding gene: 50841898

Following gene: 50841901

Centisome position: 18.01

GC content: 68.03

Gene sequence:

>1029_bases
ATGTCGATCACGCCACGCCGCCACGTTGCCGTCGGTCTGCTCTGCCGGGCTGCTGGTCTGGGGCTGGGCATTATCGCCGA
CCACATCGTGCCTGACCCGCAAACCCACCATCCGGTGGCCGTCTTCGGTGCGGCGGTCGCCCGGCTAGAAAAGACCATGT
ACGCGGATTCGACGCGACGCGGGACCCTCTTTACTGTCACCTGCTTGACGCCGCTTGCGGTGATCGGAGGCGCCGTTGAT
CGCCTCACCCGAAACCGACCGGCGCTCCGTATCGCCACCACCGCCTTGGCCACGTGGGCCGTCGTCGGGTCCGCATCTCT
GGCTCGCGAAGGCCGGGCGATGGCCGGCCATTTGGCCAATGGCGACCTGGTTGCCGCGCGGAAGCGTCTACCGCACCTGT
GTGGGCGTGCCCCTGACGCCCTCGACGCCGCCGAGATAGCCCGGGGCGCCGTCGAGTCGATGGCCGAGAACGCCTCCGAC
GCCGCAGTCGCATCGCTCTGGTGGGGCGCGGTCGCCGGGTTGCCTGGGCTGCTCGTCCACCGCGGGGCCAACACCCTTGA
CGCCATGATCGGCCACCATAACGACCGCTACGAGCACTTCGGCAAGGTGGCCGCTTACCTCGACGACGTTGTCAACTGGG
TGCCAGCTCGGTTCACCGGAGCCTTGGCCGCTGGGTGTGCCTCAACGGTCGGAGGCGATCGGAAAACTACATGGCGAGTG
GTGCGGGCCGAGCATGGCCACCATCCCAGTCCCAACGGTGGCTGGTGCGAGTCGGCCTGGGCAGCGGCCCTCGGTGTTCA
ACTCGGCGGACGTAACGTCTACTACGGCAACCGGGTGGAGTTCCGCCCCTTACTGGGGTCCGGGACCCGTCCCGATGCTG
ACAAGACGGCCGACGCGGCGCGCCTGGTCACCACGGTCACCACGGCAGCCACCTGGTGTGCTGTCGCAGGGCTGGCCAGT
GTGGCCCATGTGCTGTTCTACGGCGGCCTGATTCCGCGCGGTCTTCTGCCGCACAGCACAAGGCGTTGA

Upstream 100 bases:

>100_bases
GATTCCATGTTGACTGTCCGTGCCTGACGATGACGATGCGGCTCACCTTAGGAAGGCTACCGGGGCCGCTCGATAACTCG
ATCTCGATAAGGTGTGAGGC

Downstream 100 bases:

>100_bases
TGACAAGGAGACTCACCATGACCGGAATCCTCGTTGCCGGAACATCGTCGGACGCGGGTAAATCCCTCGTCGTTACCGCC
TTGTGCCGGGTCGCCCGACG

Product: cobalamin biosynthesis CobD/CbiB protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSITPRRHVAVGLLCRAAGLGLGIIADHIVPDPQTHHPVAVFGAAVARLEKTMYADSTRRGTLFTVTCLTPLAVIGGAVD
RLTRNRPALRIATTALATWAVVGSASLAREGRAMAGHLANGDLVAARKRLPHLCGRAPDALDAAEIARGAVESMAENASD
AAVASLWWGAVAGLPGLLVHRGANTLDAMIGHHNDRYEHFGKVAAYLDDVVNWVPARFTGALAAGCASTVGGDRKTTWRV
VRAEHGHHPSPNGGWCESAWAAALGVQLGGRNVYYGNRVEFRPLLGSGTRPDADKTADAARLVTTVTTAATWCAVAGLAS
VAHVLFYGGLIPRGLLPHSTRR

Sequences:

>Translated_342_residues
MSITPRRHVAVGLLCRAAGLGLGIIADHIVPDPQTHHPVAVFGAAVARLEKTMYADSTRRGTLFTVTCLTPLAVIGGAVD
RLTRNRPALRIATTALATWAVVGSASLAREGRAMAGHLANGDLVAARKRLPHLCGRAPDALDAAEIARGAVESMAENASD
AAVASLWWGAVAGLPGLLVHRGANTLDAMIGHHNDRYEHFGKVAAYLDDVVNWVPARFTGALAAGCASTVGGDRKTTWRV
VRAEHGHHPSPNGGWCESAWAAALGVQLGGRNVYYGNRVEFRPLLGSGTRPDADKTADAARLVTTVTTAATWCAVAGLAS
VAHVLFYGGLIPRGLLPHSTRR
>Mature_341_residues
SITPRRHVAVGLLCRAAGLGLGIIADHIVPDPQTHHPVAVFGAAVARLEKTMYADSTRRGTLFTVTCLTPLAVIGGAVDR
LTRNRPALRIATTALATWAVVGSASLAREGRAMAGHLANGDLVAARKRLPHLCGRAPDALDAAEIARGAVESMAENASDA
AVASLWWGAVAGLPGLLVHRGANTLDAMIGHHNDRYEHFGKVAAYLDDVVNWVPARFTGALAAGCASTVGGDRKTTWRVV
RAEHGHHPSPNGGWCESAWAAALGVQLGGRNVYYGNRVEFRPLLGSGTRPDADKTADAARLVTTVTTAATWCAVAGLASV
AHVLFYGGLIPRGLLPHSTRR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35843; Mature: 35712

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITPRRHVAVGLLCRAAGLGLGIIADHIVPDPQTHHPVAVFGAAVARLEKTMYADSTRR
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GTLFTVTCLTPLAVIGGAVDRLTRNRPALRIATTALATWAVVGSASLAREGRAMAGHLAN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHCCCCC
GDLVAARKRLPHLCGRAPDALDAAEIARGAVESMAENASDAAVASLWWGAVAGLPGLLVH
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHH
RGANTLDAMIGHHNDRYEHFGKVAAYLDDVVNWVPARFTGALAAGCASTVGGDRKTTWRV
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEE
VRAEHGHHPSPNGGWCESAWAAALGVQLGGRNVYYGNRVEFRPLLGSGTRPDADKTADAA
EECCCCCCCCCCCCCHHHHHHHHHHHEECCCEEEECCCEEEEECCCCCCCCCCHHHHHHH
RLVTTVTTAATWCAVAGLASVAHVLFYGGLIPRGLLPHSTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SITPRRHVAVGLLCRAAGLGLGIIADHIVPDPQTHHPVAVFGAAVARLEKTMYADSTRR
CCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GTLFTVTCLTPLAVIGGAVDRLTRNRPALRIATTALATWAVVGSASLAREGRAMAGHLAN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHCCCCC
GDLVAARKRLPHLCGRAPDALDAAEIARGAVESMAENASDAAVASLWWGAVAGLPGLLVH
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHH
RGANTLDAMIGHHNDRYEHFGKVAAYLDDVVNWVPARFTGALAAGCASTVGGDRKTTWRV
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEE
VRAEHGHHPSPNGGWCESAWAAALGVQLGGRNVYYGNRVEFRPLLGSGTRPDADKTADAA
EECCCCCCCCCCCCCHHHHHHHHHHHEECCCEEEECCCEEEEECCCCCCCCCCHHHHHHH
RLVTTVTTAATWCAVAGLASVAHVLFYGGLIPRGLLPHSTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]