Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 50841867

GI number: 50841867

Start: 431282

End: 432754

Strand: Reverse

Name: cysS

Synonym: PPA0384

Alternate gene names: 50841867

Gene position: 432754-431282 (Counterclockwise)

Preceding gene: 50841869

Following gene: 50841866

Centisome position: 16.9

GC content: 60.08

Gene sequence:

>1473_bases
ATGGTAAGTGATGTGTCAGGGTACACCGGTAGCCTAAGTCATGTGACGTTTAAGCTCTACAACACCGCTACCCATGGCAT
CGAGACCTTTAAGCCCCTTCGCGAAGGCCAGGTGTCGATTTACCACTGTGGAATGACGGTGCAGTCCAGTCCACATCTGG
GCCACATCCGCAAGGAGGTCGTCTTCGACGTCCTGCGGCGCTGGTTGGAGTGTTCTGGCTACCGGGTGACCGTTGTCGCC
AACGTCACCGATATCGACGACAAGATCCTCGCTAAGTCCGCCGCTGCCGGCACCCCGTGGTGGGCCCATGCGTACCACTA
CGAAAATGAGCTTCACGCGGCGTATAAAGCATTGGGATGCCGTCCGCCGACCTATGAGCCTCGAGCCACCGGTCACATCC
CGGAAATGATCGAGCTGGTGAAGACCCTCATCGAAAAAGGTCACGCTTATCCTGCCCCCGATGGCTCCGGTGACGTCTAC
TTCGATGTTCGATCCTGGCCGCGCTACGGGGAATTGTCCGGGATACGTGTCGACGAAATGAGCCCGGCCGAGGATTCAGA
CCCGCGTGGTAAACGCGACCCACGTGACTTTGCACTGTGGAAGGGCCACAAAGAGGACGAGCCCGAAACAGCGTCGTGGG
TGTCTCCGTGGGGACGCGGTCGACCTGGGTGGCACCTGGAATGCTCGGCGATGTCCGGGAAGTATTTGGGCGAGGCCTTC
GATATTCACGGTGGCGGTATCGACTTGCGGTTCCCTCACCATGAAAACGAGTTGGCTCAGTCAGCAGCAGCTGGGCGTCC
TTTCGCCCACTATTGGATGCACAACGCTTGGGTGACGATGTCTGGGGAGAAGATGAGCAAGTCCCTGGGTAATACCGCCC
AAGTGTCTGAGGTCACCAAGACCTACAGCCCGCGAGCCGTCAGGTACTTCCTGCTGGCCCCGCATTATCGCTCCATGATC
GAGTTCAGCCCCTCCGACGAGGGCACCATGGGCTCCCTGGATGAGGCCACCAAAGCCATCGAGCGCATCGATTCCTTTCT
GGACCGCGCCAGCAACCTCGTTGGCGAGCAGGTGACGGCTTCGCATGAATTGTCGGAGGCGTTCGTCACTGCGATGGACG
ATGACCTTGGTACTCCCCAGGCGGTGGCGGCCCTGTTCAGCCAAATCACCGAAGGTAATAAGTCCGTGTCGATCGGCGAT
GCTGAGGCCACCCGTCATCACCTGTCCCAGGTTAAAGCCATGCTCGCGGTATTCGGACTCGATCCTCAGGCCCCCGAGTG
GGCCGACGTCGCAGGCAGCGACGACCGGTTAGAGCCGGTCGTTGACGGCTTGGTGCATGCCATGCTGGAGCAGCGTGCCG
AGGCTCGTGCCCGCAAGGATTGGGCTACAGCTGACGCCATCCGCGACACCTTGACAAACCTTGGATTGACGATCGAGGAC
ACCCCCGCTGGCGCCCATTGGTCGCTGAGCTGA

Upstream 100 bases:

>100_bases
CGGGGACAACCGGCTTGATGGATTCACCCATTGCGGCCTCCAAGGCCCGGGCGATGGGGCTGGACGACGGCTTGTGCTTG
CGAATCTCGGCCATACCCCC

Downstream 100 bases:

>100_bases
CAAGGAGACTGACATGGCAAGAAATTCTCAGCGGCGCGGCGCAGGGCACAAGGGCAAGAGTTCGGCTGTTGGCTCTGGCG
GACGAGGCCGCAAGGCTCTC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MVSDVSGYTGSLSHVTFKLYNTATHGIETFKPLREGQVSIYHCGMTVQSSPHLGHIRKEVVFDVLRRWLECSGYRVTVVA
NVTDIDDKILAKSAAAGTPWWAHAYHYENELHAAYKALGCRPPTYEPRATGHIPEMIELVKTLIEKGHAYPAPDGSGDVY
FDVRSWPRYGELSGIRVDEMSPAEDSDPRGKRDPRDFALWKGHKEDEPETASWVSPWGRGRPGWHLECSAMSGKYLGEAF
DIHGGGIDLRFPHHENELAQSAAAGRPFAHYWMHNAWVTMSGEKMSKSLGNTAQVSEVTKTYSPRAVRYFLLAPHYRSMI
EFSPSDEGTMGSLDEATKAIERIDSFLDRASNLVGEQVTASHELSEAFVTAMDDDLGTPQAVAALFSQITEGNKSVSIGD
AEATRHHLSQVKAMLAVFGLDPQAPEWADVAGSDDRLEPVVDGLVHAMLEQRAEARARKDWATADAIRDTLTNLGLTIED
TPAGAHWSLS

Sequences:

>Translated_490_residues
MVSDVSGYTGSLSHVTFKLYNTATHGIETFKPLREGQVSIYHCGMTVQSSPHLGHIRKEVVFDVLRRWLECSGYRVTVVA
NVTDIDDKILAKSAAAGTPWWAHAYHYENELHAAYKALGCRPPTYEPRATGHIPEMIELVKTLIEKGHAYPAPDGSGDVY
FDVRSWPRYGELSGIRVDEMSPAEDSDPRGKRDPRDFALWKGHKEDEPETASWVSPWGRGRPGWHLECSAMSGKYLGEAF
DIHGGGIDLRFPHHENELAQSAAAGRPFAHYWMHNAWVTMSGEKMSKSLGNTAQVSEVTKTYSPRAVRYFLLAPHYRSMI
EFSPSDEGTMGSLDEATKAIERIDSFLDRASNLVGEQVTASHELSEAFVTAMDDDLGTPQAVAALFSQITEGNKSVSIGD
AEATRHHLSQVKAMLAVFGLDPQAPEWADVAGSDDRLEPVVDGLVHAMLEQRAEARARKDWATADAIRDTLTNLGLTIED
TPAGAHWSLS
>Mature_490_residues
MVSDVSGYTGSLSHVTFKLYNTATHGIETFKPLREGQVSIYHCGMTVQSSPHLGHIRKEVVFDVLRRWLECSGYRVTVVA
NVTDIDDKILAKSAAAGTPWWAHAYHYENELHAAYKALGCRPPTYEPRATGHIPEMIELVKTLIEKGHAYPAPDGSGDVY
FDVRSWPRYGELSGIRVDEMSPAEDSDPRGKRDPRDFALWKGHKEDEPETASWVSPWGRGRPGWHLECSAMSGKYLGEAF
DIHGGGIDLRFPHHENELAQSAAAGRPFAHYWMHNAWVTMSGEKMSKSLGNTAQVSEVTKTYSPRAVRYFLLAPHYRSMI
EFSPSDEGTMGSLDEATKAIERIDSFLDRASNLVGEQVTASHELSEAFVTAMDDDLGTPQAVAALFSQITEGNKSVSIGD
AEATRHHLSQVKAMLAVFGLDPQAPEWADVAGSDDRLEPVVDGLVHAMLEQRAEARARKDWATADAIRDTLTNLGLTIED
TPAGAHWSLS

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=480, Percent_Identity=34.375, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI10835051, Length=277, Percent_Identity=36.101083032491, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI62240992, Length=277, Percent_Identity=36.101083032491, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI21269877, Length=277, Percent_Identity=36.101083032491, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI303304987, Length=277, Percent_Identity=36.101083032491, Blast_Score=169, Evalue=7e-42,
Organism=Escherichia coli, GI1786737, Length=474, Percent_Identity=44.7257383966245, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17509797, Length=433, Percent_Identity=30.0230946882217, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17509795, Length=433, Percent_Identity=30.0230946882217, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324082, Length=428, Percent_Identity=32.4766355140187, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI19922218, Length=526, Percent_Identity=32.5095057034221, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19922364, Length=331, Percent_Identity=33.8368580060423, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PROAC (Q6AAT0)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055094.1
- ProteinModelPortal:   Q6AAT0
- SMR:   Q6AAT0
- GeneID:   2932059
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0384
- NMPDR:   fig|267747.1.peg.371
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   PACN267747:PPA0384-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54074; Mature: 54074

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 287-287

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDVSGYTGSLSHVTFKLYNTATHGIETFKPLREGQVSIYHCGMTVQSSPHLGHIRKEV
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECEEECCCCCHHHHHHHH
VFDVLRRWLECSGYRVTVVANVTDIDDKILAKSAAAGTPWWAHAYHYENELHAAYKALGC
HHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
RPPTYEPRATGHIPEMIELVKTLIEKGHAYPAPDGSGDVYFDVRSWPRYGELSGIRVDEM
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCC
SPAEDSDPRGKRDPRDFALWKGHKEDEPETASWVSPWGRGRPGWHLECSAMSGKYLGEAF
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCEE
DIHGGGIDLRFPHHENELAQSAAAGRPFAHYWMHNAWVTMSGEKMSKSLGNTAQVSEVTK
EECCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCEEEECCHHHHHHCCCHHHHHHHHH
TYSPRAVRYFLLAPHYRSMIEFSPSDEGTMGSLDEATKAIERIDSFLDRASNLVGEQVTA
HCCCCCEEEEEECCHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHELSEAFVTAMDDDLGTPQAVAALFSQITEGNKSVSIGDAEATRHHLSQVKAMLAVFGL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCC
DPQAPEWADVAGSDDRLEPVVDGLVHAMLEQRAEARARKDWATADAIRDTLTNLGLTIED
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
TPAGAHWSLS
CCCCCCCCCC
>Mature Secondary Structure
MVSDVSGYTGSLSHVTFKLYNTATHGIETFKPLREGQVSIYHCGMTVQSSPHLGHIRKEV
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECEEECCCCCHHHHHHHH
VFDVLRRWLECSGYRVTVVANVTDIDDKILAKSAAAGTPWWAHAYHYENELHAAYKALGC
HHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
RPPTYEPRATGHIPEMIELVKTLIEKGHAYPAPDGSGDVYFDVRSWPRYGELSGIRVDEM
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCC
SPAEDSDPRGKRDPRDFALWKGHKEDEPETASWVSPWGRGRPGWHLECSAMSGKYLGEAF
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCEE
DIHGGGIDLRFPHHENELAQSAAAGRPFAHYWMHNAWVTMSGEKMSKSLGNTAQVSEVTK
EECCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCEEEECCHHHHHHCCCHHHHHHHHH
TYSPRAVRYFLLAPHYRSMIEFSPSDEGTMGSLDEATKAIERIDSFLDRASNLVGEQVTA
HCCCCCEEEEEECCHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHELSEAFVTAMDDDLGTPQAVAALFSQITEGNKSVSIGDAEATRHHLSQVKAMLAVFGL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCC
DPQAPEWADVAGSDDRLEPVVDGLVHAMLEQRAEARARKDWATADAIRDTLTNLGLTIED
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
TPAGAHWSLS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA