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The map label for this gene is wcaJ [H]

Identifier: 50841835

GI number: 50841835

Start: 399997

End: 401382

Strand: Direct

Name: wcaJ [H]

Synonym: PPA0349

Alternate gene names: 50841835

Gene position: 399997-401382 (Clockwise)

Preceding gene: 50841834

Following gene: 50841839

Centisome position: 15.62

GC content: 51.88

Gene sequence:

>1386_bases
GTGAAGAAGGATTATTTCAACCGCACCCAGAAATTGGTGACGGTCCTCATTGACGCCATCGTCTTTGTGGTCGGCATCTT
TTTGTCATTCTGGATGCGTTTCGGAATGGTCATTCCGCAGCGAAATCTAGATGACGGAAAAGCCGCGCTCATGGCGTCGG
TTATTGCGTTCCTTATTATCAATGTCTTGTCTGGTGTTTACGTCCTGTATAACAAGACATTGTTAGATATAGGAATTATC
ACGGTTGTCGACCAGGTCATGGTGACTATCGTGATTATGGCGTTGACCTTCTTTGGGCGATGGTTTGCCTTCCCGCGGTC
GGTCCTTCTCATCAACTTGGTGGTCGGCATCGTCTTGCTCCTTGTGTGGCGCAGTGTCGAGGTCGTAGCCTATCGCCATC
TACGAGGGGCTAAGCGGGTGATGTTGCTCGGTCCTCCTGAGATGCTTAACCGGGCCGTCATGAACTACATGGCGAATAAG
GCGACCCGTCATCGCCTTACTCATGTGGTGCGTGGCCACTACTTGGAGCAGATTCGATCTCATATCAACGAGTTTGATAC
TGCCCACGTTTCTGACGATATTCCTTCCTCTGAGCGAGTCGCTATTTATGATCTGCTGCTGAGTGAGGATAAGGAAATCT
TCATGTCCACCTCGTTTGAGCATATGATGCTCATCAATCCGACAATAATGTCGATTGAAGATGAGACGATCATTGCTGCG
AGCCCATTTAAGATTCCGGCAGAAATGGATATTGTCAAGAGGTTCTTTGACATTTTACTGTCGCTAATCGCCCTGGTTGT
CGCGTCGCCAATTATGTTCGTCACCGCGATCCTGGTGAAAGCCAGCTCTCCCGGTCCGGTTTTCTACCGTCAGACCCGCA
TCACTAAGGACGGGCGCGAGTTCAAGATCCTCAAATTCCGTTCTATGGGTGTGATGGCCGAAAAAGAGTCCGGCCCCATG
TTGGCCACTACCAACGACCCGCGCGTGACCACGGTGGGCAAATACTTGCGTAGCTTGCGCATCGACGAGATCCCGCAGCT
CATTAACGTGCTCATCGGCGACATGTCGATCGTCGGTCCTCGCCCCGAACGACCCTTCTTCGTCGACCAGTTCCAAGAGC
AGAACGCCCACTACACATTGCGGCACAACGTGCGCGCTGGCATCACCGGGTACGCGCAGGTCTACGGTAAATACGCTTCA
GACTTCGCCGCCAAACTCAACTTCGATCTTATTTACATCAAGCAGTACTCACTTGTTCTGGACGTCAAGATCATGATCCA
GACCATCAAGATCCTCTTCGACAAGGTCTCTTCGCGAGGTGTTGACGAGACAGCTGAGCCCGACGAGAACATCCACCTGC
CCGCGGAGGTCCAGCAGCTGAACTGA

Upstream 100 bases:

>100_bases
GCGGGTCAGCAGCCCCTCGTCGATCCTGAAGTTGGCTTGCACGTCTGCCCGGTAGTGTGAGTCTGTGCCCCTGTGCCCAC
CAGGGTCGAGGAGGAAGTAC

Downstream 100 bases:

>100_bases
CGGGCAGGGGAGTCCTCCTGAATCAGGATTCGCGTGCCACCTTGTACTGGGCGGCCTGGGCGCGAGGACGGACGATCATT
CGATCAATGTTGACGTGGCT

Product: putative glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKDYFNRTQKLVTVLIDAIVFVVGIFLSFWMRFGMVIPQRNLDDGKAALMASVIAFLIINVLSGVYVLYNKTLLDIGII
TVVDQVMVTIVIMALTFFGRWFAFPRSVLLINLVVGIVLLLVWRSVEVVAYRHLRGAKRVMLLGPPEMLNRAVMNYMANK
ATRHRLTHVVRGHYLEQIRSHINEFDTAHVSDDIPSSERVAIYDLLLSEDKEIFMSTSFEHMMLINPTIMSIEDETIIAA
SPFKIPAEMDIVKRFFDILLSLIALVVASPIMFVTAILVKASSPGPVFYRQTRITKDGREFKILKFRSMGVMAEKESGPM
LATTNDPRVTTVGKYLRSLRIDEIPQLINVLIGDMSIVGPRPERPFFVDQFQEQNAHYTLRHNVRAGITGYAQVYGKYAS
DFAAKLNFDLIYIKQYSLVLDVKIMIQTIKILFDKVSSRGVDETAEPDENIHLPAEVQQLN

Sequences:

>Translated_461_residues
MKKDYFNRTQKLVTVLIDAIVFVVGIFLSFWMRFGMVIPQRNLDDGKAALMASVIAFLIINVLSGVYVLYNKTLLDIGII
TVVDQVMVTIVIMALTFFGRWFAFPRSVLLINLVVGIVLLLVWRSVEVVAYRHLRGAKRVMLLGPPEMLNRAVMNYMANK
ATRHRLTHVVRGHYLEQIRSHINEFDTAHVSDDIPSSERVAIYDLLLSEDKEIFMSTSFEHMMLINPTIMSIEDETIIAA
SPFKIPAEMDIVKRFFDILLSLIALVVASPIMFVTAILVKASSPGPVFYRQTRITKDGREFKILKFRSMGVMAEKESGPM
LATTNDPRVTTVGKYLRSLRIDEIPQLINVLIGDMSIVGPRPERPFFVDQFQEQNAHYTLRHNVRAGITGYAQVYGKYAS
DFAAKLNFDLIYIKQYSLVLDVKIMIQTIKILFDKVSSRGVDETAEPDENIHLPAEVQQLN
>Mature_461_residues
MKKDYFNRTQKLVTVLIDAIVFVVGIFLSFWMRFGMVIPQRNLDDGKAALMASVIAFLIINVLSGVYVLYNKTLLDIGII
TVVDQVMVTIVIMALTFFGRWFAFPRSVLLINLVVGIVLLLVWRSVEVVAYRHLRGAKRVMLLGPPEMLNRAVMNYMANK
ATRHRLTHVVRGHYLEQIRSHINEFDTAHVSDDIPSSERVAIYDLLLSEDKEIFMSTSFEHMMLINPTIMSIEDETIIAA
SPFKIPAEMDIVKRFFDILLSLIALVVASPIMFVTAILVKASSPGPVFYRQTRITKDGREFKILKFRSMGVMAEKESGPM
LATTNDPRVTTVGKYLRSLRIDEIPQLINVLIGDMSIVGPRPERPFFVDQFQEQNAHYTLRHNVRAGITGYAQVYGKYAS
DFAAKLNFDLIYIKQYSLVLDVKIMIQTIKILFDKVSSRGVDETAEPDENIHLPAEVQQLN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=184, Percent_Identity=46.7391304347826, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52386; Mature: 52386

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDYFNRTQKLVTVLIDAIVFVVGIFLSFWMRFGMVIPQRNLDDGKAALMASVIAFLII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NVLSGVYVLYNKTLLDIGIITVVDQVMVTIVIMALTFFGRWFAFPRSVLLINLVVGIVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVWRSVEVVAYRHLRGAKRVMLLGPPEMLNRAVMNYMANKATRHRLTHVVRGHYLEQIRS
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HINEFDTAHVSDDIPSSERVAIYDLLLSEDKEIFMSTSFEHMMLINPTIMSIEDETIIAA
HHHHHCCCCCCCCCCCCCCEEHHHHHHCCCCHHHHCCCCCEEEEECCEEEEECCCEEEEE
SPFKIPAEMDIVKRFFDILLSLIALVVASPIMFVTAILVKASSPGPVFYRQTRITKDGRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
FKILKFRSMGVMAEKESGPMLATTNDPRVTTVGKYLRSLRIDEIPQLINVLIGDMSIVGP
EEEEEEECCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEECC
RPERPFFVDQFQEQNAHYTLRHNVRAGITGYAQVYGKYASDFAAKLNFDLIYIKQYSLVL
CCCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHH
DVKIMIQTIKILFDKVSSRGVDETAEPDENIHLPAEVQQLN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MKKDYFNRTQKLVTVLIDAIVFVVGIFLSFWMRFGMVIPQRNLDDGKAALMASVIAFLII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NVLSGVYVLYNKTLLDIGIITVVDQVMVTIVIMALTFFGRWFAFPRSVLLINLVVGIVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVWRSVEVVAYRHLRGAKRVMLLGPPEMLNRAVMNYMANKATRHRLTHVVRGHYLEQIRS
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HINEFDTAHVSDDIPSSERVAIYDLLLSEDKEIFMSTSFEHMMLINPTIMSIEDETIIAA
HHHHHCCCCCCCCCCCCCCEEHHHHHHCCCCHHHHCCCCCEEEEECCEEEEECCCEEEEE
SPFKIPAEMDIVKRFFDILLSLIALVVASPIMFVTAILVKASSPGPVFYRQTRITKDGRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
FKILKFRSMGVMAEKESGPMLATTNDPRVTTVGKYLRSLRIDEIPQLINVLIGDMSIVGP
EEEEEEECCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEECC
RPERPFFVDQFQEQNAHYTLRHNVRAGITGYAQVYGKYASDFAAKLNFDLIYIKQYSLVL
CCCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHH
DVKIMIQTIKILFDKVSSRGVDETAEPDENIHLPAEVQQLN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]