Click here to switch to the map view.

The map label for this gene is mutY [H]

Identifier: 50841788

GI number: 50841788

Start: 350176

End: 351051

Strand: Reverse

Name: mutY [H]

Synonym: PPA0300

Alternate gene names: 50841788

Gene position: 351051-350176 (Counterclockwise)

Preceding gene: 50841789

Following gene: 50841787

Centisome position: 13.71

GC content: 63.7

Gene sequence:

>876_bases
GTGCCTGACACCGGGCTGACTTCGCACGTCATCGAGGCAATCTGTGCCTGGTTCGACGCCAACGGACGCGATCTGCCGTG
GCGCCGACCCGGCACCTCCGCGTGGGGCGTACTTGTTAGCGAGGTCATGAGCCAACAGACCCCGATGTCCCGGGTGATCG
GACCGTGGCACGAGTGGATGAACCGCTGGCCCACCCCTGATGATTTAGCGGAGGAGGACTCTGGGGAAGCAGTTGCCGCG
TGGGGGCGCCTGGGTTACCCGCGTCGGGCCTTGCGCCTGCATTCCTGTGCCGTCACGATCGCCACCGAGCACGACGGGGT
TGTGCCCAACAGTTACGACGAGCTCGTCGCCCTCCCGGGTATTGGCGACTACACCGCTAGCGCAGTCGTCTCTTTTGCGT
TTGGCGGCCGCGCCACAGTGCTTGACACCAATGTACGTCGCCTCATTGCTAGAGCAGAGTCTGGGATCGCAAACTGTCCA
ACCTCGGTGACAAGAGCTGAGCGGATAGTCGCCGACGCGTTGGTTCCCGACGAAGACGCCCGAGCGGCCAAGTGGGCGGT
GGCGTCGATGGAATTGGGGGCACTGGTATGCACGGCGCGGTCTCCGCAGTGTGAGGTCTGCCCGATCCGGGATGGCTGCC
GGTGGGTGATCGACGGTAGGCCGGACAATGCCCCGGCCCGTCGAGGACAGCCATGGAAGGGCACGGATCGCCAGTGCCGC
GGCGTGATTATGGACGTGGTGCGCAACAGCCCTCACGGAGTGAAGGTCCAGATGGCTCTTTCCGCCTGGCCCGAGCTCGA
TCAGGCATCAAGGTGCCTGGAATCCTTACTCGATGACGGTTTAGTGCACCGACGAGGTAACCTTATTAGCCTGTGA

Upstream 100 bases:

>100_bases
TTTGAGGCCTGGTTCCTCTCGGACGCTCACGACGACGAAGCTTTCGAGGTTTTCCGCGCCGCCCTGCCGAGGGCTGCCCA
GGCGGCTGCCCAGGTGATCA

Downstream 100 bases:

>100_bases
CCTGAGAAATTCTTGGACCGACCACCCAAACAGACCGAGTCCAGCAGTGATGCCGCTGGGTTATCCTTAGAGGCGGTCCT
CAAATTGGATCAGCCAAACC

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPDTGLTSHVIEAICAWFDANGRDLPWRRPGTSAWGVLVSEVMSQQTPMSRVIGPWHEWMNRWPTPDDLAEEDSGEAVAA
WGRLGYPRRALRLHSCAVTIATEHDGVVPNSYDELVALPGIGDYTASAVVSFAFGGRATVLDTNVRRLIARAESGIANCP
TSVTRAERIVADALVPDEDARAAKWAVASMELGALVCTARSPQCEVCPIRDGCRWVIDGRPDNAPARRGQPWKGTDRQCR
GVIMDVVRNSPHGVKVQMALSAWPELDQASRCLESLLDDGLVHRRGNLISL

Sequences:

>Translated_291_residues
MPDTGLTSHVIEAICAWFDANGRDLPWRRPGTSAWGVLVSEVMSQQTPMSRVIGPWHEWMNRWPTPDDLAEEDSGEAVAA
WGRLGYPRRALRLHSCAVTIATEHDGVVPNSYDELVALPGIGDYTASAVVSFAFGGRATVLDTNVRRLIARAESGIANCP
TSVTRAERIVADALVPDEDARAAKWAVASMELGALVCTARSPQCEVCPIRDGCRWVIDGRPDNAPARRGQPWKGTDRQCR
GVIMDVVRNSPHGVKVQMALSAWPELDQASRCLESLLDDGLVHRRGNLISL
>Mature_290_residues
PDTGLTSHVIEAICAWFDANGRDLPWRRPGTSAWGVLVSEVMSQQTPMSRVIGPWHEWMNRWPTPDDLAEEDSGEAVAAW
GRLGYPRRALRLHSCAVTIATEHDGVVPNSYDELVALPGIGDYTASAVVSFAFGGRATVLDTNVRRLIARAESGIANCPT
SVTRAERIVADALVPDEDARAAKWAVASMELGALVCTARSPQCEVCPIRDGCRWVIDGRPDNAPARRGQPWKGTDRQCRG
VIMDVVRNSPHGVKVQMALSAWPELDQASRCLESLLDDGLVHRRGNLISL

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298654, Length=212, Percent_Identity=39.1509433962264, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI115298652, Length=212, Percent_Identity=39.1509433962264, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI115298650, Length=212, Percent_Identity=39.1509433962264, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI115298648, Length=212, Percent_Identity=39.1509433962264, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI6912520, Length=212, Percent_Identity=39.1509433962264, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI190358497, Length=212, Percent_Identity=39.1509433962264, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1789331, Length=209, Percent_Identity=35.4066985645933, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 31756; Mature: 31625

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTGLTSHVIEAICAWFDANGRDLPWRRPGTSAWGVLVSEVMSQQTPMSRVIGPWHEWM
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHH
NRWPTPDDLAEEDSGEAVAAWGRLGYPRRALRLHSCAVTIATEHDGVVPNSYDELVALPG
HCCCCCHHHCCCCCCCEEEHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCCHHHEEECCC
IGDYTASAVVSFAFGGRATVLDTNVRRLIARAESGIANCPTSVTRAERIVADALVPDEDA
CCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
RAAKWAVASMELGALVCTARSPQCEVCPIRDGCRWVIDGRPDNAPARRGQPWKGTDRQCR
HHHHHHHHHHHHCEEEEECCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHHH
GVIMDVVRNSPHGVKVQMALSAWPELDQASRCLESLLDDGLVHRRGNLISL
HHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
PDTGLTSHVIEAICAWFDANGRDLPWRRPGTSAWGVLVSEVMSQQTPMSRVIGPWHEWM
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHH
NRWPTPDDLAEEDSGEAVAAWGRLGYPRRALRLHSCAVTIATEHDGVVPNSYDELVALPG
HCCCCCHHHCCCCCCCEEEHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCCHHHEEECCC
IGDYTASAVVSFAFGGRATVLDTNVRRLIARAESGIANCPTSVTRAERIVADALVPDEDA
CCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
RAAKWAVASMELGALVCTARSPQCEVCPIRDGCRWVIDGRPDNAPARRGQPWKGTDRQCR
HHHHHHHHHHHHCEEEEECCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHHH
GVIMDVVRNSPHGVKVQMALSAWPELDQASRCLESLLDDGLVHRRGNLISL
HHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]