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The map label for this gene is treB [H]

Identifier: 50365249

GI number: 50365249

Start: 509047

End: 510591

Strand: Reverse

Name: treB [H]

Synonym: Mfl431

Alternate gene names: 50365249

Gene position: 510591-509047 (Counterclockwise)

Preceding gene: 50365250

Following gene: 50365248

Centisome position: 64.37

GC content: 27.77

Gene sequence:

>1545_bases
ATGAAATTTACAAAAGAAAATATTGTATTTCTTTTAGAAAATGTTGGTGGATCTGAAAATATTGAAAAAATAAGTCACTG
TATTTCTAGAATGAGATTTAAACTTGTAGATATTACAAAAGCTAACACTGAGGAAATCAAAAAGCAAAAATGATGTAAAG
GTGTTTTAATTGTTGGTGGTGAATATCAAGTTGTTATAGGAACAGAAATTGAAAGTTTTTATAAAATGTTTTTAAAAACA
ACTGGAATAAATGAAAACGCAAATGATGAGTTAAAAACTAAAAAACAAAAAATGACTTTTATTATGTTTGTTTCATCAAT
TTTTAGCCCAATAATGATTTTGCTTGTTGTATACGGAATGTGAGAAATGGTAAGAACGCCTTTCTTCTTATCTTCTAATT
CAACAGAAATTCCAACATTAATTGAAATTAATAGTTTTATGGAATTAATTTCAAAAGGATTAAGTTGATTTATTGTTATG
GCAGTTTGTTGAAGTACATTTAGAGTAATGGGAGGGACACCAATTATAGGTTTAGCAATTGGCGCAATTCTTATTAGTCC
AATGTTAACACCTCTTTCAGCACTTGATCTTGATGGTGGTAAAACAATAATACAAGCAATGAATGAACATGGTTGAAAAA
TATTTGGAAACGCTGTTTTCCCATGAAAAGTTTCATTTGAAGGTTTAGTAATACCGATGATTTTTGTTGGTTTACTAGGA
GTTTTCATTGAAAGAGGGATGAATAAAGTTAATTTAGGTGGAGCTAGAATGCTTATTCAACCAATGGTTGTTATTGCTGG
AACAGTTATTGTAACTATGTTTACAATTGCTCCAGCAGGATTAATAATAACAAATTATATGTCTATATCATTTAACTTTT
TAATGACACATAATATAACAAAATATATATTCTCACCATTAATAGGAATGCTATATGCACCAATGGTAGTATTTGGATTA
CATAGAACAATAACACCTATATTAATGCAAGATATATTAAAATTTAACGGTAGTTTTATAATGGGGTTAATGATCATTTC
TAACATTTGTTTAGCTGTAGGTTGTTTAACATTTGGAAGATTAAACAAAAACTGTAAAGAAGTAAAAAATATTGCATATT
CTAATGGTATTTCTGCATTTATCGCAGGAGTAACTGAGCCTGCTATTTATTCAATTTCTTTAAAATATCTTTTCCCTTTA
ATAGCTTCTGCTATAGGAACATATTTTGGATGCTTATTGTATACTTCAGCTGGAGTTTGAACAAATGCAGCACCTTTTGG
TATTTTAGGAATAATCGGTTTTGCTTCAACTATACCACAAGGCATAAATGTAGAACAATGAGCTGGTGGTTCAATTGTTT
GAGGAACACTAAGTGTAATTTTGGGAATATCAATAACAATATTTATGACAATAGTTTTAAGTCAAGTTAAATATTTTAAA
GAAAGAACAAATAAAATACTTTTTGATGAATACGGATTTACTCCTGAATTAAAACAAAAAAGTATATTTAAATTTAAAGG
TAATAAAAATGGAAAAATTAAGTAA

Upstream 100 bases:

>100_bases
AATACTAAACTAGTTTTTAGAAAACAAACTAGTTTAGTATTTTATTTTACTTTAGCATTTTTATAAAAAATGAGTTGAAA
TATATACAAAGGAGAATTTT

Downstream 100 bases:

>100_bases
AGATATAATGTTGGGAACTTCAATATCAGCTAATCAAGCAGAAGGTTCATGAAATATTAATGGTAAAGGTTTATCAATAG
CTGAAATGCGTAGATATAAT

Product: trehalose/sucrose/beta-glucoside PTS system IIBC component

Products: NA

Alternate protein names: EIIBC-Tre; EII-Tre; Trehalose-specific phosphotransferase enzyme IIB component; PTS system trehalose-specific EIIB component; Trehalose permease IIC component; PTS system trehalose-specific EIIC component [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKFTKENIVFLLENVGGSENIEKISHCISRMRFKLVDITKANTEEIKKQKWCKGVLIVGGEYQVVIGTEIESFYKMFLKT
TGINENANDELKTKKQKMTFIMFVSSIFSPIMILLVVYGMWEMVRTPFFLSSNSTEIPTLIEINSFMELISKGLSWFIVM
AVCWSTFRVMGGTPIIGLAIGAILISPMLTPLSALDLDGGKTIIQAMNEHGWKIFGNAVFPWKVSFEGLVIPMIFVGLLG
VFIERGMNKVNLGGARMLIQPMVVIAGTVIVTMFTIAPAGLIITNYMSISFNFLMTHNITKYIFSPLIGMLYAPMVVFGL
HRTITPILMQDILKFNGSFIMGLMIISNICLAVGCLTFGRLNKNCKEVKNIAYSNGISAFIAGVTEPAIYSISLKYLFPL
IASAIGTYFGCLLYTSAGVWTNAAPFGILGIIGFASTIPQGINVEQWAGGSIVWGTLSVILGISITIFMTIVLSQVKYFK
ERTNKILFDEYGFTPELKQKSIFKFKGNKNGKIK

Sequences:

>Translated_514_residues
MKFTKENIVFLLENVGGSENIEKISHCISRMRFKLVDITKANTEEIKKQK*CKGVLIVGGEYQVVIGTEIESFYKMFLKT
TGINENANDELKTKKQKMTFIMFVSSIFSPIMILLVVYGM*EMVRTPFFLSSNSTEIPTLIEINSFMELISKGLS*FIVM
AVC*STFRVMGGTPIIGLAIGAILISPMLTPLSALDLDGGKTIIQAMNEHG*KIFGNAVFP*KVSFEGLVIPMIFVGLLG
VFIERGMNKVNLGGARMLIQPMVVIAGTVIVTMFTIAPAGLIITNYMSISFNFLMTHNITKYIFSPLIGMLYAPMVVFGL
HRTITPILMQDILKFNGSFIMGLMIISNICLAVGCLTFGRLNKNCKEVKNIAYSNGISAFIAGVTEPAIYSISLKYLFPL
IASAIGTYFGCLLYTSAGV*TNAAPFGILGIIGFASTIPQGINVEQ*AGGSIV*GTLSVILGISITIFMTIVLSQVKYFK
ERTNKILFDEYGFTPELKQKSIFKFKGNKNGKIK
>Mature_514_residues
MKFTKENIVFLLENVGGSENIEKISHCISRMRFKLVDITKANTEEIKKQK*CKGVLIVGGEYQVVIGTEIESFYKMFLKT
TGINENANDELKTKKQKMTFIMFVSSIFSPIMILLVVYGM*EMVRTPFFLSSNSTEIPTLIEINSFMELISKGLS*FIVM
AVC*STFRVMGGTPIIGLAIGAILISPMLTPLSALDLDGGKTIIQAMNEHG*KIFGNAVFP*KVSFEGLVIPMIFVGLLG
VFIERGMNKVNLGGARMLIQPMVVIAGTVIVTMFTIAPAGLIITNYMSISFNFLMTHNITKYIFSPLIGMLYAPMVVFGL
HRTITPILMQDILKFNGSFIMGLMIISNICLAVGCLTFGRLNKNCKEVKNIAYSNGISAFIAGVTEPAIYSISLKYLFPL
IASAIGTYFGCLLYTSAGV*TNAAPFGILGIIGFASTIPQGINVEQ*AGGSIV*GTLSVILGISITIFMTIVLSQVKYFK
ERTNKILFDEYGFTPELKQKSIFKFKGNKNGKIK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=448, Percent_Identity=31.4732142857143, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1790159, Length=458, Percent_Identity=23.7991266375546, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI48994906, Length=427, Percent_Identity=23.8875878220141, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011296 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 55138; Mature: 55138

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS51098 PTS_EIIB_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKENIVFLLENVGGSENIEKISHCISRMRFKLVDITKANTEEIKKQKCKGVLIVGGE
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCEEEEECCC
YQVVIGTEIESFYKMFLKTTGINENANDELKTKKQKMTFIMFVSSIFSPIMILLVVYGME
EEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVRTPFFLSSNSTEIPTLIEINSFMELISKGLSFIVMAVCSTFRVMGGTPIIGLAIGAIL
HHCCCEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ISPMLTPLSALDLDGGKTIIQAMNEHGKIFGNAVFPKVSFEGLVIPMIFVGLLGVFIERG
HHHHHCCHHHHCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
MNKVNLGGARMLIQPMVVIAGTVIVTMFTIAPAGLIITNYMSISFNFLMTHNITKYIFSP
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEHHHHHHHHHHHHH
LIGMLYAPMVVFGLHRTITPILMQDILKFNGSFIMGLMIISNICLAVGCLTFGRLNKNCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EVKNIAYSNGISAFIAGVTEPAIYSISLKYLFPLIASAIGTYFGCLLYTSAGVTNAAPFG
HHHHHHHHCCHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ILGIIGFASTIPQGINVEQAGGSIVGTLSVILGISITIFMTIVLSQVKYFKERTNKILFD
HHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
EYGFTPELKQKSIFKFKGNKNGKIK
CCCCCCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKFTKENIVFLLENVGGSENIEKISHCISRMRFKLVDITKANTEEIKKQKCKGVLIVGGE
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCEEEEECCC
YQVVIGTEIESFYKMFLKTTGINENANDELKTKKQKMTFIMFVSSIFSPIMILLVVYGME
EEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVRTPFFLSSNSTEIPTLIEINSFMELISKGLSFIVMAVCSTFRVMGGTPIIGLAIGAIL
HHCCCEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ISPMLTPLSALDLDGGKTIIQAMNEHGKIFGNAVFPKVSFEGLVIPMIFVGLLGVFIERG
HHHHHCCHHHHCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
MNKVNLGGARMLIQPMVVIAGTVIVTMFTIAPAGLIITNYMSISFNFLMTHNITKYIFSP
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEHHHHHHHHHHHHH
LIGMLYAPMVVFGLHRTITPILMQDILKFNGSFIMGLMIISNICLAVGCLTFGRLNKNCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EVKNIAYSNGISAFIAGVTEPAIYSISLKYLFPLIASAIGTYFGCLLYTSAGVTNAAPFG
HHHHHHHHCCHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ILGIIGFASTIPQGINVEQAGGSIVGTLSVILGISITIFMTIVLSQVKYFKERTNKILFD
HHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
EYGFTPELKQKSIFKFKGNKNGKIK
CCCCCCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608078; 7610040; 9278503 [H]