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The map label for this gene is thyA [H]

Identifier: 50365237

GI number: 50365237

Start: 491075

End: 491941

Strand: Reverse

Name: thyA [H]

Synonym: Mfl419

Alternate gene names: 50365237

Gene position: 491941-491075 (Counterclockwise)

Preceding gene: 50365238

Following gene: 50365236

Centisome position: 62.02

GC content: 28.14

Gene sequence:

>867_bases
ATGAAACAATATTTAGAATTAGTTAATGAAATTTTAAAAGATGGAGAAAAAAGAGAAGATCGTACAAACACAGGAACAAT
ATCTAAGTTTGGTGTACAAAAAAGATATGATTTACGTGAAGGTTTTCCATTACTAACAACTAAAAAAGTTTTTTATAAAG
CAATCTTTCATGAGATGTTATGGTTTATAAAAGGTGATACCAATATTAAATACTTGGTTGAGAATAATGTAAAAATTTGA
AATGAATGACCATATGAAAATTTTAAAAAATCATCTGAGTTCAATAATGAGACTTTACAAGAGTTTGTTGAAAGATTAAA
GAATGATAATGAGTTCTGTAATAAATGAGGAGATTTAGGACCTGTTTATGGAAAACAATGAAGAGACTTTGGTGGAATTG
ATCAATTTGCAAAACTAATTAATGATATTAAGAAAAATCCATTTTCAAGAAGACATATTGTTTCAGCATGAAATCCAGCT
GAAGTTGATAACATGTTGTTACCACCATGTCATTCTTTTTGACAAGTTTATGTTTCAAAAGACGGTTGATTAGATTTACA
ACTATACCAAAGAAGTGGTGATGTGTTTTTAGGAGTGCCATTTAATATAGCATCGTATGCGTTACTAATGGAATTAATCG
CAAAAGAATGTAACTTAAAAGCAAGATACTTTGTTCATACAATTGGGGATGCTCATATTTATTTAAACCATCTAGAACAA
ATTAATGAGCAATTAAAAAGAAAACCATTGCCACTATGCAAAATTAAGATTAATTCTGAAAAATCAATTTTTGATATTGC
ATTTGAGGACATTGAAATTGAAGGTTATGAAAGTCATGCTAAAATAACAGGAGAAGTTGCAGTTTAG

Upstream 100 bases:

>100_bases
GGAAAAATATATTTAGAAAAAAATAAAAATCAACATATTTAGAAAAGAAAATTAATAATTTTAGAATTATAATCTTTACA
TTGAAATAAGGAGATTGTAT

Downstream 100 bases:

>100_bases
TAACTTTTTCTTTATTTTAAGAATTAGCACTCTATTATTGACTTTGCTAAAATTATGTATTAATATAATTACAAACCTAG
GAAAAAGGTTTTTATCAAGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKQYLELVNEILKDGEKREDRTNTGTISKFGVQKRYDLREGFPLLTTKKVFYKAIFHEMLWFIKGDTNIKYLVENNVKIW
NEWPYENFKKSSEFNNETLQEFVERLKNDNEFCNKWGDLGPVYGKQWRDFGGIDQFAKLINDIKKNPFSRRHIVSAWNPA
EVDNMLLPPCHSFWQVYVSKDGWLDLQLYQRSGDVFLGVPFNIASYALLMELIAKECNLKARYFVHTIGDAHIYLNHLEQ
INEQLKRKPLPLCKIKINSEKSIFDIAFEDIEIEGYESHAKITGEVAV

Sequences:

>Translated_288_residues
MKQYLELVNEILKDGEKREDRTNTGTISKFGVQKRYDLREGFPLLTTKKVFYKAIFHEMLWFIKGDTNIKYLVENNVKI*
NE*PYENFKKSSEFNNETLQEFVERLKNDNEFCNK*GDLGPVYGKQ*RDFGGIDQFAKLINDIKKNPFSRRHIVSA*NPA
EVDNMLLPPCHSF*QVYVSKDG*LDLQLYQRSGDVFLGVPFNIASYALLMELIAKECNLKARYFVHTIGDAHIYLNHLEQ
INEQLKRKPLPLCKIKINSEKSIFDIAFEDIEIEGYESHAKITGEVAV
>Mature_288_residues
MKQYLELVNEILKDGEKREDRTNTGTISKFGVQKRYDLREGFPLLTTKKVFYKAIFHEMLWFIKGDTNIKYLVENNVKI*
NE*PYENFKKSSEFNNETLQEFVERLKNDNEFCNK*GDLGPVYGKQ*RDFGGIDQFAKLINDIKKNPFSRRHIVSA*NPA
EVDNMLLPPCHSF*QVYVSKDG*LDLQLYQRSGDVFLGVPFNIASYALLMELIAKECNLKARYFVHTIGDAHIYLNHLEQ
INEQLKRKPLPLCKIKINSEKSIFDIAFEDIEIEGYESHAKITGEVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=296, Percent_Identity=47.2972972972973, Blast_Score=247, Evalue=9e-66,
Organism=Escherichia coli, GI1789191, Length=290, Percent_Identity=48.6206896551724, Blast_Score=264, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71993377, Length=297, Percent_Identity=45.7912457912458, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324648, Length=306, Percent_Identity=36.6013071895425, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137556, Length=300, Percent_Identity=45.6666666666667, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32460; Mature: 32460

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLELVNEILKDGEKREDRTNTGTISKFGVQKRYDLREGFPLLTTKKVFYKAIFHEML
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
WFIKGDTNIKYLVENNVKINEPYENFKKSSEFNNETLQEFVERLKNDNEFCNKGDLGPVY
HHEECCCCEEEEEECCEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
GKQRDFGGIDQFAKLINDIKKNPFSRRHIVSANPAEVDNMLLPPCHSFQVYVSKDGLDLQ
CCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHCCCCCCCCCCEEEEEECCCCEEE
LYQRSGDVFLGVPFNIASYALLMELIAKECNLKARYFVHTIGDAHIYLNHLEQINEQLKR
EEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
KPLPLCKIKINSEKSIFDIAFEDIEIEGYESHAKITGEVAV
CCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCEEEEEECC
>Mature Secondary Structure
MKQYLELVNEILKDGEKREDRTNTGTISKFGVQKRYDLREGFPLLTTKKVFYKAIFHEML
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
WFIKGDTNIKYLVENNVKINEPYENFKKSSEFNNETLQEFVERLKNDNEFCNKGDLGPVY
HHEECCCCEEEEEECCEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
GKQRDFGGIDQFAKLINDIKKNPFSRRHIVSANPAEVDNMLLPPCHSFQVYVSKDGLDLQ
CCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHCCCCCCCCCCEEEEEECCCCEEE
LYQRSGDVFLGVPFNIASYALLMELIAKECNLKARYFVHTIGDAHIYLNHLEQINEQLKR
EEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
KPLPLCKIKINSEKSIFDIAFEDIEIEGYESHAKITGEVAV
CCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA