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The map label for this gene is ruvB [H]

Identifier: 50365238

GI number: 50365238

Start: 492000

End: 492947

Strand: Reverse

Name: ruvB [H]

Synonym: Mfl420

Alternate gene names: 50365238

Gene position: 492947-492000 (Counterclockwise)

Preceding gene: 50365239

Following gene: 50365237

Centisome position: 62.14

GC content: 23.52

Gene sequence:

>948_bases
ATGAGTAATGATTTTAGACCTAGTAAGTGGAGTGAATACATAGGACAAGAAAAGGTCATTAAGAATTTAAAAATATGTAT
AGAATCATCAATCAAACAAAATAAAGTTTTAGATCCAATAATTTTCTCAGGACCAAGTGGAATGGGTAAAACAAGCTTAG
CTTATTTATTAAGCAAAATATTAAAAACTAAAATTCATATTGTTAATGGGCCTAGTTTACAAAGACCAAGTGATTTGATT
TCAGTTCTAACAAGTATAAAAGAAAATCAAATTCTTTTTATTGATGAAGTTCATTCAGTTAGCAAAGATATCATGGAAGT
GTTATATCCGGTTTTAGAAGAAAATAAACTTAGTATTATTATTGGAAAAGAATATAATTCTAAAATAGTTAATATAAAGT
TACCAAACTTTACTATCATAACTGCAACAACAGAAATAAATAAATTACCATTTCCTTTTTTGAATAGATTTCCAATACAA
TTCGAATTAGAGGGTTACAATCATGATGATTTAACAAAAATTATAATTAATACATTTAATAAATTGCAATACAAAATAGA
AATTGAACAAGCAAAGATTATTGCTAAATTTTCTCGATTAGTTCCAAGAGTTGCTATAAATTTAGTAAAAAGAATTTATG
ATTTTTTGATAACAGAAAAAATAAAAGATTTATCAGAAAAAAATTTAATATGAGTTTTTAAACAAATGGGTCTTTATGAA
TATGGTTTAAATGAAAAAGATTTAGACTACTTATTTACACTATTAGAAAATAATACATTGTCATTAGATAGCTTAGCGCA
AATTATAAATGTACCTAATCAAACAATTTTAAATAATATGGAGCCAATCTTTTTAAAAGAAAGATTAATTATAAAAACAG
GCCGTGGTAGACAATTAACTAACAAAGGAAAAATATATTTAGAAAAAAATAAAAATCAACATATTTAG

Upstream 100 bases:

>100_bases
CTGGGATTTTCTTATAAAACAATAATGAAAAATATTGCTAATGTTGATAATTTACTTTCAGTGGATGAAATAACCAAAAT
AGTTATGGAGCAAATTAGTT

Downstream 100 bases:

>100_bases
AAAAGAAAATTAATAATTTTAGAATTATAATCTTTACATTGAAATAAGGAGATTGTATATGAAACAATATTTAGAATTAG
TTAATGAAATTTTAAAAGAT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSNDFRPSKWSEYIGQEKVIKNLKICIESSIKQNKVLDPIIFSGPSGMGKTSLAYLLSKILKTKIHIVNGPSLQRPSDLI
SVLTSIKENQILFIDEVHSVSKDIMEVLYPVLEENKLSIIIGKEYNSKIVNIKLPNFTIITATTEINKLPFPFLNRFPIQ
FELEGYNHDDLTKIIINTFNKLQYKIEIEQAKIIAKFSRLVPRVAINLVKRIYDFLITEKIKDLSEKNLIWVFKQMGLYE
YGLNEKDLDYLFTLLENNTLSLDSLAQIINVPNQTILNNMEPIFLKERLIIKTGRGRQLTNKGKIYLEKNKNQHI

Sequences:

>Translated_315_residues
MSNDFRPSKWSEYIGQEKVIKNLKICIESSIKQNKVLDPIIFSGPSGMGKTSLAYLLSKILKTKIHIVNGPSLQRPSDLI
SVLTSIKENQILFIDEVHSVSKDIMEVLYPVLEENKLSIIIGKEYNSKIVNIKLPNFTIITATTEINKLPFPFLNRFPIQ
FELEGYNHDDLTKIIINTFNKLQYKIEIEQAKIIAKFSRLVPRVAINLVKRIYDFLITEKIKDLSEKNLI*VFKQMGLYE
YGLNEKDLDYLFTLLENNTLSLDSLAQIINVPNQTILNNMEPIFLKERLIIKTGRGRQLTNKGKIYLEKNKNQHI
>Mature_314_residues
SNDFRPSKWSEYIGQEKVIKNLKICIESSIKQNKVLDPIIFSGPSGMGKTSLAYLLSKILKTKIHIVNGPSLQRPSDLIS
VLTSIKENQILFIDEVHSVSKDIMEVLYPVLEENKLSIIIGKEYNSKIVNIKLPNFTIITATTEINKLPFPFLNRFPIQF
ELEGYNHDDLTKIIINTFNKLQYKIEIEQAKIIAKFSRLVPRVAINLVKRIYDFLITEKIKDLSEKNLI*VFKQMGLYEY
GLNEKDLDYLFTLLENNTLSLDSLAQIINVPNQTILNNMEPIFLKERLIIKTGRGRQLTNKGKIYLEKNKNQHI

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=301, Percent_Identity=30.2325581395349, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36225; Mature: 36094

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFRPSKWSEYIGQEKVIKNLKICIESSIKQNKVLDPIIFSGPSGMGKTSLAYLLSKI
CCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHH
LKTKIHIVNGPSLQRPSDLISVLTSIKENQILFIDEVHSVSKDIMEVLYPVLEENKLSII
HHHEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEE
IGKEYNSKIVNIKLPNFTIITATTEINKLPFPFLNRFPIQFELEGYNHDDLTKIIINTFN
EECCCCCEEEEEECCCEEEEEEECCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHH
KLQYKIEIEQAKIIAKFSRLVPRVAINLVKRIYDFLITEKIKDLSEKNLIVFKQMGLYEY
HEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHC
GLNEKDLDYLFTLLENNTLSLDSLAQIINVPNQTILNNMEPIFLKERLIIKTGRGRQLTN
CCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCEEECEEEEECCCCCEECC
KGKIYLEKNKNQHI
CCCEEEECCCCCCC
>Mature Secondary Structure 
SNDFRPSKWSEYIGQEKVIKNLKICIESSIKQNKVLDPIIFSGPSGMGKTSLAYLLSKI
CCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHH
LKTKIHIVNGPSLQRPSDLISVLTSIKENQILFIDEVHSVSKDIMEVLYPVLEENKLSII
HHHEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEE
IGKEYNSKIVNIKLPNFTIITATTEINKLPFPFLNRFPIQFELEGYNHDDLTKIIINTFN
EECCCCCEEEEEECCCEEEEEEECCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHH
KLQYKIEIEQAKIIAKFSRLVPRVAINLVKRIYDFLITEKIKDLSEKNLIVFKQMGLYEY
HEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHC
GLNEKDLDYLFTLLENNTLSLDSLAQIINVPNQTILNNMEPIFLKERLIIKTGRGRQLTN
CCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCEEECEEEEECCCCCEECC
KGKIYLEKNKNQHI
CCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA