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The map label for this gene is clpB [H]

Identifier: 50365236

GI number: 50365236

Start: 488827

End: 490962

Strand: Reverse

Name: clpB [H]

Synonym: Mfl418

Alternate gene names: 50365236

Gene position: 490962-488827 (Counterclockwise)

Preceding gene: 50365237

Following gene: 50365235

Centisome position: 61.89

GC content: 28.98

Gene sequence:

>2136_bases
ATGGAATTTCAACAAAAAGGTGAAGAAATTAACGCATTAGAAAAGTACACACGTGATTTAACTCAAAGTGCTAAGGATGG
AAAAATTGATCCTATTATTGGTAGAGAAGAAGAAATATTGAGAGTTATTAGAATTCTTTCAAGAAAAACAAAAAACAATC
CTGTTTTAATTGGAGAACCCGGAGTTGGTAAAACTGCAGTAGTTGAAGGATTAGCTCAAAGAATAGTTAAAGGTGATGTG
CCTTCAATTTTAAAAGGAAAAAGAATTTTAGAATTAGATATGGGAAGCTTAATGGCTGGTGCTATGTATCTTGGGGACTA
TGAGTCTAGGGTAAAAGCAATTGTTAATGAAATCAAAAAATCAGAAGGTGAAATAATTTTATTCATTGACGAACTTCATT
TAATAGTTGGTGCTGGTAAAACAGGTAATGGTGGAGGAATGGATGTTTCAAACCTTCTAAAACCAGCTCTTGCAAGAGGA
GAGTTAAAAGCTATTGGAGCTACAACTTTAAAAGAATATAGAGAATACATTGAAAAAGATTCTGCATTAGAAAGAAGATT
TCAAAGAGTAATGGTAGAAGAACCAACTGTTGATCAAACTATTTCTATTCTTAGAGGATTAAAAGAAAGATTTGAAACTT
ATCATGGAGTTAGAATTCATGATAATGCAATTGTAAGTGCTGCAAATTTATCAAATAGATATATAGCTGATAGATTCTTA
CCAGATAAAGCAATTGATTTAATTGATGAAGCATCTTCTACAATTAAAACTGAATTAGCAAGTGTACCTACTGAACTAGA
TCAAGTTAATCGTAAAGTTATGCAACTTGAAATTGAAAAAAGTGCACTTTCAAAAGAAAAAGACGATAAATCAAAAGAAC
GTTTAGCTGAAGCTGTTAAAGAACTTGAAAAAACAAAAATTGAACAAGATAAATTAGCAAGCGAATGAAATAAACAAAAA
TCTCAATTAGAAAAAATAAATAGTTTCAAAACAACTATTGAAAACTTAAAAAATGAATTAGAAATTTCACAGAATGAAGG
TAATTATAAACGTGCTGGAGAAATACAATATTCATTATTACCAAGTTTAGAAAAACAATTAAGTGAAGCTGAAAAAGATA
CTCGTGAAACATTAGTTAGTGAAGAAGTTACTGAAAAAGATGTTGCTGAAATTGTTGCTAAATGAACAGGTATTCCAGTT
GATAGACTAGTTGAGTCTGAAAAAACAAAATTACTTGAGTTAGAAAAAACTTTAGAAGAAAGAGTAAAAGGACAAAATCA
AGCAATCGAAGCTGTTTCTCAGGCTATCTTGAGAAGCAGAAGTGGTATTAAAGATCCCAATAAACCAATTGGTTCATTCT
TATTCTTAGGTCCAACAGGTGTTGGTAAGACTGAAGTTGCAAGAAGTTTAGCTGATATATTATTTAACTCAAATAAAAAA
ATGGTAAGAATAGATATGAGTGAATACATGGAAAAACATAGTGTATCTAAATTAATTGGTTCTCCTCCAGGTTATGTTGG
GTATGAAGAAGGTGGAAGATTAACTGAAGCTGTTAGAAGAAATCCGTACTCAATTGTTCTATTTGATGAAATTGAAAAAG
CGCACCCTGATGTATTTAACATTTTATTGCAAGTTTTGGATGAAGGAAGAATTACAGACTCACTAGGTAAAACAGTAGAC
TTCAAAAACACAATTATCATATTAACTTCAAATCTAGGTAGTGAATATATTTTAAAAAATGGTTCAACATCTGAAGAAAG
TAAAATAGCTATTCAAGCAGAATTGCTAAAACATTTTAGACCTGAATTTTTAAATAGAATTGATAATGTTGTTGTATTTA
ATCCATTGTCAAAAGAAAATGTTTCTGAAATAGTAACAAAAGAACTAAATACTTTATCAGCAAGATTAGAAAATGATAAA
AATTTCTTTTTAACATTTGATATGAAAGCAAAAAATAAAATAATTGATGAAGGATATGACTCTCAATTCGGAGCAAGACC
AATAAAAAGATATATTGAAAAAAATATTGAAACATTATTAGCTCAAAAAATAATTTCTGGTGAAATAGAAGAAAACATTA
GATATACAATAACAGTTGAAAATGATGACTTTGAATTAACAAAAATTAGAAATTAA

Upstream 100 bases:

>100_bases
TATTTTAAGAATTAGCACTCTATTATTGACTTTGCTAAAATTATGTATTAATATAATTACAAACCTAGGAAAAAGGTTTT
TATCAAGGAGGAAATTTATT

Downstream 100 bases:

>100_bases
GAAAAAAAGTAGCAATCTAATGTATAGAGTGCTAAAATAAAAATAGGTGATCAAATGTTAAGCGAAAGACAAGCTAAAAT
TTTAAAAGTAATTGTTTCAG

Product: ATPase subunit of ATP-dependant protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MEFQQKGEEINALEKYTRDLTQSAKDGKIDPIIGREEEILRVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDV
PSILKGKRILELDMGSLMAGAMYLGDYESRVKAIVNEIKKSEGEIILFIDELHLIVGAGKTGNGGGMDVSNLLKPALARG
ELKAIGATTLKEYREYIEKDSALERRFQRVMVEEPTVDQTISILRGLKERFETYHGVRIHDNAIVSAANLSNRYIADRFL
PDKAIDLIDEASSTIKTELASVPTELDQVNRKVMQLEIEKSALSKEKDDKSKERLAEAVKELEKTKIEQDKLASEWNKQK
SQLEKINSFKTTIENLKNELEISQNEGNYKRAGEIQYSLLPSLEKQLSEAEKDTRETLVSEEVTEKDVAEIVAKWTGIPV
DRLVESEKTKLLELEKTLEERVKGQNQAIEAVSQAILRSRSGIKDPNKPIGSFLFLGPTGVGKTEVARSLADILFNSNKK
MVRIDMSEYMEKHSVSKLIGSPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHPDVFNILLQVLDEGRITDSLGKTVD
FKNTIIILTSNLGSEYILKNGSTSEESKIAIQAELLKHFRPEFLNRIDNVVVFNPLSKENVSEIVTKELNTLSARLENDK
NFFLTFDMKAKNKIIDEGYDSQFGARPIKRYIEKNIETLLAQKIISGEIEENIRYTITVENDDFELTKIRN

Sequences:

>Translated_711_residues
MEFQQKGEEINALEKYTRDLTQSAKDGKIDPIIGREEEILRVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDV
PSILKGKRILELDMGSLMAGAMYLGDYESRVKAIVNEIKKSEGEIILFIDELHLIVGAGKTGNGGGMDVSNLLKPALARG
ELKAIGATTLKEYREYIEKDSALERRFQRVMVEEPTVDQTISILRGLKERFETYHGVRIHDNAIVSAANLSNRYIADRFL
PDKAIDLIDEASSTIKTELASVPTELDQVNRKVMQLEIEKSALSKEKDDKSKERLAEAVKELEKTKIEQDKLASE*NKQK
SQLEKINSFKTTIENLKNELEISQNEGNYKRAGEIQYSLLPSLEKQLSEAEKDTRETLVSEEVTEKDVAEIVAK*TGIPV
DRLVESEKTKLLELEKTLEERVKGQNQAIEAVSQAILRSRSGIKDPNKPIGSFLFLGPTGVGKTEVARSLADILFNSNKK
MVRIDMSEYMEKHSVSKLIGSPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHPDVFNILLQVLDEGRITDSLGKTVD
FKNTIIILTSNLGSEYILKNGSTSEESKIAIQAELLKHFRPEFLNRIDNVVVFNPLSKENVSEIVTKELNTLSARLENDK
NFFLTFDMKAKNKIIDEGYDSQFGARPIKRYIEKNIETLLAQKIISGEIEENIRYTITVENDDFELTKIRN
>Mature_711_residues
MEFQQKGEEINALEKYTRDLTQSAKDGKIDPIIGREEEILRVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDV
PSILKGKRILELDMGSLMAGAMYLGDYESRVKAIVNEIKKSEGEIILFIDELHLIVGAGKTGNGGGMDVSNLLKPALARG
ELKAIGATTLKEYREYIEKDSALERRFQRVMVEEPTVDQTISILRGLKERFETYHGVRIHDNAIVSAANLSNRYIADRFL
PDKAIDLIDEASSTIKTELASVPTELDQVNRKVMQLEIEKSALSKEKDDKSKERLAEAVKELEKTKIEQDKLASE*NKQK
SQLEKINSFKTTIENLKNELEISQNEGNYKRAGEIQYSLLPSLEKQLSEAEKDTRETLVSEEVTEKDVAEIVAK*TGIPV
DRLVESEKTKLLELEKTLEERVKGQNQAIEAVSQAILRSRSGIKDPNKPIGSFLFLGPTGVGKTEVARSLADILFNSNKK
MVRIDMSEYMEKHSVSKLIGSPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHPDVFNILLQVLDEGRITDSLGKTVD
FKNTIIILTSNLGSEYILKNGSTSEESKIAIQAELLKHFRPEFLNRIDNVVVFNPLSKENVSEIVTKELNTLSARLENDK
NFFLTFDMKAKNKIIDEGYDSQFGARPIKRYIEKNIETLLAQKIISGEIEENIRYTITVENDDFELTKIRN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=328, Percent_Identity=39.3292682926829, Blast_Score=228, Evalue=1e-59,
Organism=Escherichia coli, GI1788943, Length=686, Percent_Identity=56.7055393586006, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=327, Percent_Identity=41.2844036697248, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=49.5063469675599, Blast_Score=677, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=692, Percent_Identity=45.0867052023121, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 79700; Mature: 79700

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFQQKGEEINALEKYTRDLTQSAKDGKIDPIIGREEEILRVIRILSRKTKNNPVLIGEP
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECC
GVGKTAVVEGLAQRIVKGDVPSILKGKRILELDMGSLMAGAMYLGDYESRVKAIVNEIKK
CCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHH
SEGEIILFIDELHLIVGAGKTGNGGGMDVSNLLKPALARGELKAIGATTLKEYREYIEKD
CCCCEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SALERRFQRVMVEEPTVDQTISILRGLKERFETYHGVRIHDNAIVSAANLSNRYIADRFL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCEEEHHCCCCHHHHHHCC
PDKAIDLIDEASSTIKTELASVPTELDQVNRKVMQLEIEKSALSKEKDDKSKERLAEAVK
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ELEKTKIEQDKLASENKQKSQLEKINSFKTTIENLKNELEISQNEGNYKRAGEIQYSLLP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHH
SLEKQLSEAEKDTRETLVSEEVTEKDVAEIVAKTGIPVDRLVESEKTKLLELEKTLEERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KGQNQAIEAVSQAILRSRSGIKDPNKPIGSFLFLGPTGVGKTEVARSLADILFNSNKKMV
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEE
RIDMSEYMEKHSVSKLIGSPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHPDVFNIL
EECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHH
LQVLDEGRITDSLGKTVDFKNTIIILTSNLGSEYILKNGSTSEESKIAIQAELLKHFRPE
HHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHCHH
FLNRIDNVVVFNPLSKENVSEIVTKELNTLSARLENDKNFFLTFDMKAKNKIIDEGYDSQ
HHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCCC
FGARPIKRYIEKNIETLLAQKIISGEIEENIRYTITVENDDFELTKIRN
CCCHHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEECCCEEEEECCC
>Mature Secondary Structure
MEFQQKGEEINALEKYTRDLTQSAKDGKIDPIIGREEEILRVIRILSRKTKNNPVLIGEP
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECC
GVGKTAVVEGLAQRIVKGDVPSILKGKRILELDMGSLMAGAMYLGDYESRVKAIVNEIKK
CCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHH
SEGEIILFIDELHLIVGAGKTGNGGGMDVSNLLKPALARGELKAIGATTLKEYREYIEKD
CCCCEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SALERRFQRVMVEEPTVDQTISILRGLKERFETYHGVRIHDNAIVSAANLSNRYIADRFL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCEEEHHCCCCHHHHHHCC
PDKAIDLIDEASSTIKTELASVPTELDQVNRKVMQLEIEKSALSKEKDDKSKERLAEAVK
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ELEKTKIEQDKLASENKQKSQLEKINSFKTTIENLKNELEISQNEGNYKRAGEIQYSLLP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHH
SLEKQLSEAEKDTRETLVSEEVTEKDVAEIVAKTGIPVDRLVESEKTKLLELEKTLEERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KGQNQAIEAVSQAILRSRSGIKDPNKPIGSFLFLGPTGVGKTEVARSLADILFNSNKKMV
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEE
RIDMSEYMEKHSVSKLIGSPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHPDVFNIL
EECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHH
LQVLDEGRITDSLGKTVDFKNTIIILTSNLGSEYILKNGSTSEESKIAIQAELLKHFRPE
HHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHCHH
FLNRIDNVVVFNPLSKENVSEIVTKELNTLSARLENDKNFFLTFDMKAKNKIIDEGYDSQ
HHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCCC
FGARPIKRYIEKNIETLLAQKIISGEIEENIRYTITVENDDFELTKIRN
CCCHHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]