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The map label for this gene is lon [H]

Identifier: 50365222

GI number: 50365222

Start: 472161

End: 474524

Strand: Reverse

Name: lon [H]

Synonym: Mfl404

Alternate gene names: 50365222

Gene position: 474524-472161 (Counterclockwise)

Preceding gene: 50365223

Following gene: 50365221

Centisome position: 59.82

GC content: 28.81

Gene sequence:

>2364_bases
ATGAGTAAAAAAATAAAACTACCTATTTTCCAAATAAGAGGTTCATTTATTGTTCCTGGAATTAAAGAGAATTTAGAAGT
TGGAAGAAAAAACACATTAGCGAGCGTTAATTATGCGATTAAAAATTCAAATAATCAAATGATCGCAATACCACAGATTG
ATGCATCTGTTGAAAAACCTGAATTTTCAGATTTACATGAATTTGGAATTTTAATTGATTTTGAAGTAATCAAAGAATGA
AAAGATAATTCATTAACTATTAGCACAAACCCTATTCAAAGATGTAAAGTAATTAGCTTTTTTGAAAATGAAGATCAAGT
GCCATATGCGGAAGTTGAATTAATTGAATCAATTAATGATTTTAGTGATGAAGAGTTAAAAGAATTAATTGAAAAAATCT
CTGATGCTATCAAAACTAAAGCTAGTTTAGTAACAAAACAAATTAAACAGTTAATATCAGGTGAATCTGATGATTTAAGC
TTAGCTTTTGATTCAATTATGTTTAAACTGGCTCCATCAAAAATATTGACAAATCCAGAATACATAACATCTCCATCATT
AAAAACAAGATGATCAATTATTGAAAAAATAATTTTTGCTGAAGATGGAATCATAACAAGAAATGCAGAATCAATTGATG
CAGCAAGACAAAAGAATGAAATTGAACAAGAACTAAACCATAAATTAAAAGAAAAAATGGATAAACAACAAAAAGAATAT
TATCTAAGAGAAAAAATGAGAATTATCAAAGATGAATTAGAAGATGAAGATGATTCTGATGACAGTTCATTAGAAAAATA
TAAAGAACGTTTAGCTAAAGAACCTTTCCCTGAAGAAGTCAAAAGAAAAATTATGGCTTCTATCAAAAGAGTTGAAGCAC
TTCAATCAGGAACTCCTGAATGAAATACTGAGAAAAATTACATTGATTGAATGATGAGTATTCCTTGATGAGAAGAAACT
GAAGACTTAACTGATTTAAAATATGCTAAAAAAATTTTAGACAAACATCATTATGGTATGAAAAAAGTTAAAGAAAGAAT
TATTGAGTACTTAGCTGTTAAAACTAAAACAAAATCACTAAAAGCACCAATTATTACATTAGTTGGTCCCCCAGGTGTAG
GTAAAACAAGTTTAGCAAAATCAATTGCAGAAGCAGTTGGAAAAAACTTTGTTAAAGTAAGTTTAGGTGGAGTTAAAGAT
GAATCAGAAATTCGTGGACATAGAAAAACTTATGTTGGTTCAATGCCAGGAAGAATTATTCAAACAATGAAAAGAGCAAA
AGTAAAAAACCCATTATTCTTGCTTGATGAAATTGATAAAATGGCATCTGATCACAGAGGTGATCCAGCAAGTGCAATGT
TAGAGGTTCTTGACCCAGAACAAAACAAAGAGTTTTCAGATCATTATATTGAAGAACCATATGATCTAAGCCAAGTTATG
TTTATAGCAACTGCTAACTATCCTGAAGATATTCCTGAAGCCTTATATGACCGTATGGAAATCATAAATTTATCTAGCTA
TACTGAAATTGAAAAAGTTAAGATAGCACAAGATTATTTAGTTCCAAAAGCAATAGAACAACATGAATTAACTTCAGAAG
AAATATCATTTACTGAAGGTGCAATTAATGAAATTATTAAATATTACACTAGAGAAGCTGGTGTGCGTCAATTAGAAAGA
CATATTAATTCTATTATTAGAAAGTATATTGTTAAAAATCTAAATGGTGAAATGGATAAAATTGTTATTGATGAAAAACA
AGTAAATGATTTATTAGGTAAAAGAATTTTTGATCACACTGAAAAACAAGAAGAATCTCAAATTGGTGTGGTTACAGGTT
TAGCATATACACAATTTGGTGGAGATATTTTACCAATTGAAGTAAGTTTATACCCTGGTAAAGGAAATTTAATTCTTACA
GGTAAACTTGGTGAAGTTATGAAAGAATCTGCAACAATTGCGTTAACTTATGTTAAATCAAACTTTGAAAAATTTGGAGT
AGATAAAAAAGTATTTGAAGAAAATGATATTCATGTTCACGTTCCTGAAGGAGCTGTTCCAAAAGATGGACCAAGTGCTG
GTATCACAATTACAACTGCTTTAATTTCTGCACTTTCAGATAAACCAGTTTCAAAAGAAATTGGAATGACTGGTGAAATC
ACACTAAGAGGAAATGTATTGCCAATTGGTGGATTAAGAGAAAAATCAATTTCTGCTTCAAGAAGTGGATTGAAAACAAT
CATTATTCCAAAGAAAAATGAAAGAGACTTAGACGAAATTCCAGATGAAGTAAAAGCAAAACTAAAAATTATACCTGCTG
AAAAATATGAAGAGGTATTTGCAATAGTTTTTAAAACAAAATAA

Upstream 100 bases:

>100_bases
AAAACAAACCTGATAAAGGTTTTTTTATTTTTAGCACTCTACTATTGTAATTGCTAAACAATATGATAATATATATTTAA
CCACATAGGAGGTTAACAAA

Downstream 100 bases:

>100_bases
GAGTAAAATAATAACTAGTAAAAACTAGTTATTTTTTATTAAGGAGGAATTATGAATACACCATTAGCATATTTATTAAG
ACCAAAGACAACATCTGAAA

Product: class III heat shock DNA-binding ATP dependent Lon protease

Products: NA

Alternate protein names: ATP-dependent protease La [H]

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNNQMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKEW
KDNSLTISTNPIQRCKVISFFENEDQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLS
LAFDSIMFKLAPSKILTNPEYITSPSLKTRWSIIEKIIFAEDGIITRNAESIDAARQKNEIEQELNHKLKEKMDKQQKEY
YLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVKRKIMASIKRVEALQSGTPEWNTEKNYIDWMMSIPWWEET
EDLTDLKYAKKILDKHHYGMKKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVKD
ESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSQVM
FIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQDYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLER
HINSIIRKYIVKNLNGEMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKGNLILT
GKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAGITITTALISALSDKPVSKEIGMTGEI
TLRGNVLPIGGLREKSISASRSGLKTIIIPKKNERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK

Sequences:

>Translated_787_residues
MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNNQMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKE*
KDNSLTISTNPIQRCKVISFFENEDQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLS
LAFDSIMFKLAPSKILTNPEYITSPSLKTR*SIIEKIIFAEDGIITRNAESIDAARQKNEIEQELNHKLKEKMDKQQKEY
YLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVKRKIMASIKRVEALQSGTPE*NTEKNYID*MMSIP**EET
EDLTDLKYAKKILDKHHYGMKKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVKD
ESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSQVM
FIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQDYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLER
HINSIIRKYIVKNLNGEMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKGNLILT
GKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAGITITTALISALSDKPVSKEIGMTGEI
TLRGNVLPIGGLREKSISASRSGLKTIIIPKKNERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK
>Mature_786_residues
SKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNNQMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKE*K
DNSLTISTNPIQRCKVISFFENEDQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSL
AFDSIMFKLAPSKILTNPEYITSPSLKTR*SIIEKIIFAEDGIITRNAESIDAARQKNEIEQELNHKLKEKMDKQQKEYY
LREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVKRKIMASIKRVEALQSGTPE*NTEKNYID*MMSIP**EETE
DLTDLKYAKKILDKHHYGMKKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVKDE
SEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSQVMF
IATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQDYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLERH
INSIIRKYIVKNLNGEMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKGNLILTG
KLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAGITITTALISALSDKPVSKEIGMTGEIT
LRGNVLPIGGLREKSISASRSGLKTIIIPKKNERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK

Specific function: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced

COG id: COG0466

COG function: function code O; ATP-dependent Lon protease, bacterial type

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Lon domain [H]

Homologues:

Organism=Homo sapiens, GI31377667, Length=554, Percent_Identity=44.4043321299639, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI21396489, Length=576, Percent_Identity=41.6666666666667, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI1786643, Length=787, Percent_Identity=43.0749682337992, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17505831, Length=609, Percent_Identity=39.9014778325123, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17556486, Length=535, Percent_Identity=40.3738317757009, Blast_Score=426, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319449, Length=626, Percent_Identity=39.9361022364217, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24666867, Length=578, Percent_Identity=43.598615916955, Blast_Score=500, Evalue=1e-141,
Organism=Drosophila melanogaster, GI221513036, Length=578, Percent_Identity=43.598615916955, Blast_Score=499, Evalue=1e-141,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008269
- InterPro:   IPR004815
- InterPro:   IPR003111
- InterPro:   IPR008268
- InterPro:   IPR001984
- InterPro:   IPR015947
- InterPro:   IPR020568 [H]

Pfam domain/function: PF00004 AAA; PF02190 LON; PF05362 Lon_C [H]

EC number: =3.4.21.53 [H]

Molecular weight: Translated: 88058; Mature: 87927

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01046 LON_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNNQMIAIPQIDASVEKP
CCCCCCCCCEEECCCEECCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCC
EFSDLHEFGILIDFEVIKEKDNSLTISTNPIQRCKVISFFENEDQVPYAEVELIESINDF
CHHHHHHHCCEEEEHHHHCCCCEEEEECCCHHHHEEEEEECCCCCCCHHHHHHHHHHCCC
SDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAFDSIMFKLAPSKILTNPEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCC
ITSPSLKTRSIIEKIIFAEDGIITRNAESIDAARQKNEIEQELNHKLKEKMDKQQKEYYL
CCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVKRKIMASIKRVEALQSGTPENT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
EKNYIDMMSIPEETEDLTDLKYAKKILDKHHYGMKKVKERIIEYLAVKTKTKSLKAPIIT
CCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
LVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVKDESEIRGHRKTYVGSMPGRIIQTMKRA
EECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHCCCCCHHHHHHHHHH
KVKNPLFLLDEIDKMASDHRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSQVMFIATAN
HHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEEECC
YPEDIPEALYDRMEIINLSSYTEIEKVKIAQDYLVPKAIEQHELTSEEISFTEGAINEII
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHH
KYYTREAGVRQLERHINSIIRKYIVKNLNGEMDKIVIDEKQVNDLLGKRIFDHTEKQEES
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHC
QIGVVTGLAYTQFGGDILPIEVSLYPGKGNLILTGKLGEVMKESATIALTYVKSNFEKFG
CCEEEEEHHHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHCCCEEEEEEEHHHHHHHC
VDKKVFEENDIHVHVPEGAVPKDGPSAGITITTALISALSDKPVSKEIGMTGEITLRGNV
CCHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCEEEEEECCE
LPIGGLREKSISASRSGLKTIIIPKKNERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKT
EECCCCCHHHHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHEEEECHHHHHHHHHEEEEC
K
C
>Mature Secondary Structure 
SKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNNQMIAIPQIDASVEKP
CCCCCCCCEEECCCEECCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCC
EFSDLHEFGILIDFEVIKEKDNSLTISTNPIQRCKVISFFENEDQVPYAEVELIESINDF
CHHHHHHHCCEEEEHHHHCCCCEEEEECCCHHHHEEEEEECCCCCCCHHHHHHHHHHCCC
SDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAFDSIMFKLAPSKILTNPEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCC
ITSPSLKTRSIIEKIIFAEDGIITRNAESIDAARQKNEIEQELNHKLKEKMDKQQKEYYL
CCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVKRKIMASIKRVEALQSGTPENT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
EKNYIDMMSIPEETEDLTDLKYAKKILDKHHYGMKKVKERIIEYLAVKTKTKSLKAPIIT
CCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
LVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVKDESEIRGHRKTYVGSMPGRIIQTMKRA
EECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHCCCCCHHHHHHHHHH
KVKNPLFLLDEIDKMASDHRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSQVMFIATAN
HHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEEECC
YPEDIPEALYDRMEIINLSSYTEIEKVKIAQDYLVPKAIEQHELTSEEISFTEGAINEII
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHH
KYYTREAGVRQLERHINSIIRKYIVKNLNGEMDKIVIDEKQVNDLLGKRIFDHTEKQEES
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHC
QIGVVTGLAYTQFGGDILPIEVSLYPGKGNLILTGKLGEVMKESATIALTYVKSNFEKFG
CCEEEEEHHHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHCCCEEEEEEEHHHHHHHC
VDKKVFEENDIHVHVPEGAVPKDGPSAGITITTALISALSDKPVSKEIGMTGEITLRGNV
CCHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCEEEEEECCE
LPIGGLREKSISASRSGLKTIIIPKKNERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKT
EECCCCCHHHHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHEEEECHHHHHHHHHEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA