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The map label for this gene is tig [H]

Identifier: 50365223

GI number: 50365223

Start: 474634

End: 475914

Strand: Reverse

Name: tig [H]

Synonym: Mfl405

Alternate gene names: 50365223

Gene position: 475914-474634 (Counterclockwise)

Preceding gene: 50365224

Following gene: 50365222

Centisome position: 60.0

GC content: 28.26

Gene sequence:

>1281_bases
ATGAAATTCACAGAATTAAAAATTATTGAACAAGGTCAAGGTAAATGAACTGTTACTATTGATGGAACAGAATGAACAGA
AACTTTAAAAAAAGCAAAAAACAGAGTTCTTGCTAACTTAGAAGTTCCTGGATTTAGAAAAGGAAAAATTCCTGCTGCTC
AAGCTGAAAAATATGTAACTCCTTCAAAAATTTATAATGAAGCTTATAGAATGATGGTTTCACCTGCTTTTGACTTTGCA
AGAGCTCAAGAAGTTAAAGTTGAACCAATGAACTCACCAGAACCAATTCCAGCAAAAGTAAGTGAAAAAGAATTAGTTAT
TGAATTCTTATTTGACTTAAAACCTGAAATTAAATTAGGTGATTACAAAAACATTAAAACAGTTAAAAAAGAAACTGTTG
AAGTTACAAAAGAAGAAATTGAAGCAGTTATTGATCAATACTGTGAACAATTCATTATGGAAAAACCAAAAGCTGCTGAT
GCAAAAATTGAAAAAGGTGATATTGTTACTTTTGACTTTAAAGGATTTATGAATGGAGAAGCTTTCAAAGGTGGAGAAGC
TAAAGGGCATAAATTAGTTATAGGTTCAAACCAATTCATTCCTGGATTTGAAGATTCAATGATCGGTTTAGGACTTGGAG
AAGCTAAAATTGATGTAACTTTCCCAGAAGGTTATACTCCAGAATTAGCAAACAAACCTGCAACTTTTGAATTAAACATT
ATTGAAGTTAAAGCAAGAGAATTACCTAAAAAAGATGACGAACTTGTTAAAGATTTAAATTTACCTAATGTTGAAACTTT
TGCTCAATTTGAAGCAAAAGTAAAAGAAGACATTACAAAACAAAAATTACAAAATGTTAAAAATCAATTCGTTAATGATT
TAATTAATGAAATAATTAAAAATTCAACAATCGAATTGCCTAAAACAGCAATTGAAAACCAAACAGCAGATTTAAGAAAA
GAATTTGAAGCTCAATTAAAACAACAAGGATTAGATATTAAAAAATATAAAAAAGTTACTGGTTTATCAGATGAAGCTAT
TAAAGCTGAATTAACAGCTGATGCTAAAAATAAATTAGAAACTTACTTAGTTACATCTGAAATTAGAAGTAAAGAAAAAT
TTGAAGTTACTGAAGAAGCAATCAATGCTAAATTCGAAAACTTAGCAGCGCAATTTGGAATTCCTGCTGATCAAATTAAA
ACTATGGTAAATCCTGAAATGTTAAAATCAGAAATTGTTAACGATTTATTAGTTGATTTCTTATATTCAAATAACGGATA
A

Upstream 100 bases:

>100_bases
ATTAATAAAATTGTTGAAGAAATAATTGTAAAAACATTAAGCATAAAGCCTAAAAATATAGCTGTTGCTTATAAGAGATA
GAATAAACGAGGAATAGAAA

Downstream 100 bases:

>100_bases
TTATAAAATAAAACAAACCTGATAAAGGTTTTTTTATTTTTAGCACTCTACTATTGTAATTGCTAAACAATATGATAATA
TATATTTAACCACATAGGAG

Product: trigger factor, prolyl isomerase

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKFTELKIIEQGQGKWTVTIDGTEWTETLKKAKNRVLANLEVPGFRKGKIPAAQAEKYVTPSKIYNEAYRMMVSPAFDFA
RAQEVKVEPMNSPEPIPAKVSEKELVIEFLFDLKPEIKLGDYKNIKTVKKETVEVTKEEIEAVIDQYCEQFIMEKPKAAD
AKIEKGDIVTFDFKGFMNGEAFKGGEAKGHKLVIGSNQFIPGFEDSMIGLGLGEAKIDVTFPEGYTPELANKPATFELNI
IEVKARELPKKDDELVKDLNLPNVETFAQFEAKVKEDITKQKLQNVKNQFVNDLINEIIKNSTIELPKTAIENQTADLRK
EFEAQLKQQGLDIKKYKKVTGLSDEAIKAELTADAKNKLETYLVTSEIRSKEKFEVTEEAINAKFENLAAQFGIPADQIK
TMVNPEMLKSEIVNDLLVDFLYSNNG

Sequences:

>Translated_426_residues
MKFTELKIIEQGQGK*TVTIDGTE*TETLKKAKNRVLANLEVPGFRKGKIPAAQAEKYVTPSKIYNEAYRMMVSPAFDFA
RAQEVKVEPMNSPEPIPAKVSEKELVIEFLFDLKPEIKLGDYKNIKTVKKETVEVTKEEIEAVIDQYCEQFIMEKPKAAD
AKIEKGDIVTFDFKGFMNGEAFKGGEAKGHKLVIGSNQFIPGFEDSMIGLGLGEAKIDVTFPEGYTPELANKPATFELNI
IEVKARELPKKDDELVKDLNLPNVETFAQFEAKVKEDITKQKLQNVKNQFVNDLINEIIKNSTIELPKTAIENQTADLRK
EFEAQLKQQGLDIKKYKKVTGLSDEAIKAELTADAKNKLETYLVTSEIRSKEKFEVTEEAINAKFENLAAQFGIPADQIK
TMVNPEMLKSEIVNDLLVDFLYSNNG
>Mature_426_residues
MKFTELKIIEQGQGK*TVTIDGTE*TETLKKAKNRVLANLEVPGFRKGKIPAAQAEKYVTPSKIYNEAYRMMVSPAFDFA
RAQEVKVEPMNSPEPIPAKVSEKELVIEFLFDLKPEIKLGDYKNIKTVKKETVEVTKEEIEAVIDQYCEQFIMEKPKAAD
AKIEKGDIVTFDFKGFMNGEAFKGGEAKGHKLVIGSNQFIPGFEDSMIGLGLGEAKIDVTFPEGYTPELANKPATFELNI
IEVKARELPKKDDELVKDLNLPNVETFAQFEAKVKEDITKQKLQNVKNQFVNDLINEIIKNSTIELPKTAIENQTADLRK
EFEAQLKQQGLDIKKYKKVTGLSDEAIKAELTADAKNKLETYLVTSEIRSKEKFEVTEEAINAKFENLAAQFGIPADQIK
TMVNPEMLKSEIVNDLLVDFLYSNNG

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=318, Percent_Identity=31.7610062893082, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 47654; Mature: 47654

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTELKIIEQGQGKTVTIDGTETETLKKAKNRVLANLEVPGFRKGKIPAAQAEKYVTPS
CCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCHH
KIYNEAYRMMVSPAFDFARAQEVKVEPMNSPEPIPAKVSEKELVIEFLFDLKPEIKLGDY
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
KNIKTVKKETVEVTKEEIEAVIDQYCEQFIMEKPKAADAKIEKGDIVTFDFKGFMNGEAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCCCCCCC
KGGEAKGHKLVIGSNQFIPGFEDSMIGLGLGEAKIDVTFPEGYTPELANKPATFELNIIE
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCHHHCCCCCEEEEEEEE
VKARELPKKDDELVKDLNLPNVETFAQFEAKVKEDITKQKLQNVKNQFVNDLINEIIKNS
EEHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TIELPKTAIENQTADLRKEFEAQLKQQGLDIKKYKKVTGLSDEAIKAELTADAKNKLETY
CCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LVTSEIRSKEKFEVTEEAINAKFENLAAQFGIPADQIKTMVNPEMLKSEIVNDLLVDFLY
EEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
SNNG
CCCC
>Mature Secondary Structure
MKFTELKIIEQGQGKTVTIDGTETETLKKAKNRVLANLEVPGFRKGKIPAAQAEKYVTPS
CCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCHH
KIYNEAYRMMVSPAFDFARAQEVKVEPMNSPEPIPAKVSEKELVIEFLFDLKPEIKLGDY
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
KNIKTVKKETVEVTKEEIEAVIDQYCEQFIMEKPKAADAKIEKGDIVTFDFKGFMNGEAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCCCCCCC
KGGEAKGHKLVIGSNQFIPGFEDSMIGLGLGEAKIDVTFPEGYTPELANKPATFELNIIE
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCHHHCCCCCEEEEEEEE
VKARELPKKDDELVKDLNLPNVETFAQFEAKVKEDITKQKLQNVKNQFVNDLINEIIKNS
EEHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TIELPKTAIENQTADLRKEFEAQLKQQGLDIKKYKKVTGLSDEAIKAELTADAKNKLETY
CCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LVTSEIRSKEKFEVTEEAINAKFENLAAQFGIPADQIKTMVNPEMLKSEIVNDLLVDFLY
EEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
SNNG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA