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The map label for this gene is 50365220

Identifier: 50365220

GI number: 50365220

Start: 470174

End: 470854

Strand: Reverse

Name: 50365220

Synonym: Mfl402

Alternate gene names: NA

Gene position: 470854-470174 (Counterclockwise)

Preceding gene: 50365221

Following gene: 50365219

Centisome position: 59.36

GC content: 26.58

Gene sequence:

>681_bases
GTGAAAAAGATACTTATTGTATTAACTAATACAAACCAATATGGTAATTACAAGGAAAAAACTGGATTGTGGCTTGGTGA
AGCAACCGAATTTATCAATTTTTTAAAAAACACTGATATTAAATTTGAATTTACTTTCATCAGCCCTAAGGGAGGAAAAG
TTCCTGTAGATCCAAGAAGCTTAAAATTTGCAAGTGAGGAAGATATTAAACTTTATAAAGATAAATGATTTGTTGATAAA
GCGTTAACTAATTCATTAAAACCAAGTGATATAAAAGACGAGGAATATGATTGTATATATTTCACAGGTGGTCACGGAGT
AATGTGAGATTTTACAAATAACAAAGAAATTCACGACATAGCTTTAAAAATTTATGAAAAAAATAAAATAGTATCTTCAG
TATGTCATGGAATCGCAGGTTTACTTTATTTAAAGGATAAGAATAATAAATTTATTATTGAAAGTAGAAATATTACAGGA
TTTACAACGACAGAAGAAATTTTAAGTGGTAAATATAGGAAGGTACCTTTTCTTAATAAAAAAATAGCAATTAAAAATAA
TGCTAATTTTAAAAGCAAAAGATTTTATAAGCCATACGCGTTAAAGGATGGAAATTTAATTACAGGACAAAACCCCTTTT
CTGTAATTAAAGTAGCTAAATTGGTTTATGAAAATATTTAA

Upstream 100 bases:

>100_bases
ATTAAATATTTCAAACCTAAAACAAACTCTGCGTATGAAAGAAAACTTTGATCTTTATATGAGGAGATGAAAAAATAATA
CTAAAAGAGGAGTTATTATA

Downstream 100 bases:

>100_bases
TTAAATACAAAGAATAACAAATTTTTAAATTCTTTTTTAAGAGTTTTTAAATTTTTTTTATTTTTTGGAAAAAATTTTTT
AAATTTGTTGCAACTTGATA

Product: putative PFPI-like cystiene protease

Products: NA

Alternate protein names: ThiJ/PfpI Domain-Containing Protein; ThiJ/PfpI Domain Protein; ThiJ/PfpI; DJ-1/PfpI Family Protein; Intracellular Protease/Amidase; DJ-1/PfpI Family; NonF-Related Protein; Peptidase; Intracellular Protease/Amidase ThiJ/PfpL Family; Exported Pfp1 Family Protein; Intracellular Protease/Amidase-Like; C56 Family Peptidase; Amidotransferase; PFPI-Like Cystiene Protease; Protease I; Intracellular Protease Amidase; ThiJ/PfpI Protein; Regulatory Protein; ThiJ Protein; NonF Protein; Chaperone Protein; Thij/Pfpi Domain-Containing Protein; Transcriptional Regulator Fis Family; Protease; Intracellular Protease/Amidase ThiJ/PfpL Family Protein; Pfp1 Family Protein; Fis Family Transcriptional Regulator

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKKILIVLTNTNQYGNYKEKTGLWLGEATEFINFLKNTDIKFEFTFISPKGGKVPVDPRSLKFASEEDIKLYKDKWFVDK
ALTNSLKPSDIKDEEYDCIYFTGGHGVMWDFTNNKEIHDIALKIYEKNKIVSSVCHGIAGLLYLKDKNNKFIIESRNITG
FTTTEEILSGKYRKVPFLNKKIAIKNNANFKSKRFYKPYALKDGNLITGQNPFSVIKVAKLVYENI

Sequences:

>Translated_226_residues
MKKILIVLTNTNQYGNYKEKTGLWLGEATEFINFLKNTDIKFEFTFISPKGGKVPVDPRSLKFASEEDIKLYKDK*FVDK
ALTNSLKPSDIKDEEYDCIYFTGGHGVM*DFTNNKEIHDIALKIYEKNKIVSSVCHGIAGLLYLKDKNNKFIIESRNITG
FTTTEEILSGKYRKVPFLNKKIAIKNNANFKSKRFYKPYALKDGNLITGQNPFSVIKVAKLVYENI
>Mature_226_residues
MKKILIVLTNTNQYGNYKEKTGLWLGEATEFINFLKNTDIKFEFTFISPKGGKVPVDPRSLKFASEEDIKLYKDK*FVDK
ALTNSLKPSDIKDEEYDCIYFTGGHGVM*DFTNNKEIHDIALKIYEKNKIVSSVCHGIAGLLYLKDKNNKFIIESRNITG
FTTTEEILSGKYRKVPFLNKKIAIKNNANFKSKRFYKPYALKDGNLITGQNPFSVIKVAKLVYENI

Specific function: Unknown

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Saccharomyces cerevisiae, GI6320742, Length=228, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324967, Length=226, Percent_Identity=30.9734513274336, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324975, Length=226, Percent_Identity=30.9734513274336, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323984, Length=225, Percent_Identity=30.6666666666667, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25627; Mature: 25627

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILIVLTNTNQYGNYKEKTGLWLGEATEFINFLKNTDIKFEFTFISPKGGKVPVDPRS
CCEEEEEEECCCCCCCCHHHCCEEEHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
LKFASEEDIKLYKDKFVDKALTNSLKPSDIKDEEYDCIYFTGGHGVMDFTNNKEIHDIAL
EECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEECCCCEEEEEEE
KIYEKNKIVSSVCHGIAGLLYLKDKNNKFIIESRNITGFTTTEEILSGKYRKVPFLNKKI
EEEHHHHHHHHHHHHHEEEEEEEECCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCEE
AIKNNANFKSKRFYKPYALKDGNLITGQNPFSVIKVAKLVYENI
EEECCCCCCCCCCCCCEEECCCCEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKILIVLTNTNQYGNYKEKTGLWLGEATEFINFLKNTDIKFEFTFISPKGGKVPVDPRS
CCEEEEEEECCCCCCCCHHHCCEEEHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
LKFASEEDIKLYKDKFVDKALTNSLKPSDIKDEEYDCIYFTGGHGVMDFTNNKEIHDIAL
EECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEECCCCEEEEEEE
KIYEKNKIVSSVCHGIAGLLYLKDKNNKFIIESRNITGFTTTEEILSGKYRKVPFLNKKI
EEEHHHHHHHHHHHHHEEEEEEEECCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCEE
AIKNNANFKSKRFYKPYALKDGNLITGQNPFSVIKVAKLVYENI
EEECCCCCCCCCCCCCEEECCCCEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA