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The map label for this gene is ribF/mnmA [H]

Identifier: 50365150

GI number: 50365150

Start: 390800

End: 391624

Strand: Reverse

Name: ribF/mnmA [H]

Synonym: Mfl334

Alternate gene names: 50365150

Gene position: 391624-390800 (Counterclockwise)

Preceding gene: 50365152

Following gene: 50365149

Centisome position: 49.37

GC content: 23.52

Gene sequence:

>825_bases
ATGACTATACACTATAATGAAATGCTAATGATGCTATGAAATTTGCCTCAAACAGTAGCTGTATTTGCAGAATTTGATAA
ATGAGATGAAAATGAAACTAATTTAATTAAAAAAGCGAAAGAGAACAATTTAAAAGTTATTTTGATTCAAAAAGAAATTA
GTAACTTTAAAAATATAATTCCAAATAAAGGAATTGAGCAAAAAGCCAAAGATTTAGATTGTGAACTTGTTTTATGACAT
TCATTTAATCAAGAATATAATTATGAAAGAATTAAACAAGATTTAAATATTCAAAAAGTTTATTTATTTGATAACAATTC
AGATCTAGCCAAAATTGAAACAGCTTTTGAAGATAAAACAATTATTAAATATGATGAAAAACTTAATAATGATATTAAAA
AGAGTGAAGAGTTTTTAAAATTAGGAAAAATTCAAGAATATAAAAATTTAAATGGTTTTAATTTTGGTTTTAAAGGTTTT
GTTGAGCATGGTAACCACATTGGGAGAACAATTAATTATCCAACTGCAAATATCATTATTAATGATGAGTTGATTTTGAA
AGAAGCTGTTTATTTAACAACTACTAAATTACCAAATGACGAAAAAGCATATCCAAGTATTAGCGCTTATTGAACTAATA
AAAATGGAGTTAATGTATTTGAGAGTCATATTTTAGATTTTGATCAAGATATTTATGGATGAGGAATCGATGTAGAAATT
ATTGATTTTATACGTGAAAGTATTAAAGTAAATAACTTAGACGAACTGAAAGATTTATTAAATCAAGATAAAGCATATGC
AATAAAACATTTTAAAGGAGAATAA

Upstream 100 bases:

>100_bases
TATAATGTAAAATAGAAATAAATTAATAATTTAGTACAACATTAATTTGAATTTTAAAAATATAATTAAATTAAATTGTA
TTAAGTGGGGGAGAAACAAA

Downstream 100 bases:

>100_bases
AAAATGAAAAAACCAATTTTAGGTAGCCATGTTGGTATGAGCAAATCAAATAAACATGGAGAATATTTAATCGGAAGTGT
AAAAGAAGCTTTAAGTTATG

Product: riboflavin kinase

Products: NA

Alternate protein names: FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase; Riboflavin kinase; Flavokinase; tRNA-specific 2-thiouridylase mnmA [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTIHYNEMLMMLWNLPQTVAVFAEFDKWDENETNLIKKAKENNLKVILIQKEISNFKNIIPNKGIEQKAKDLDCELVLWH
SFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIKYDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGF
VEHGNHIGRTINYPTANIIINDELILKEAVYLTTTKLPNDEKAYPSISAYWTNKNGVNVFESHILDFDQDIYGWGIDVEI
IDFIRESIKVNNLDELKDLLNQDKAYAIKHFKGE

Sequences:

>Translated_274_residues
MTIHYNEMLMML*NLPQTVAVFAEFDK*DENETNLIKKAKENNLKVILIQKEISNFKNIIPNKGIEQKAKDLDCELVL*H
SFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIKYDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGF
VEHGNHIGRTINYPTANIIINDELILKEAVYLTTTKLPNDEKAYPSISAY*TNKNGVNVFESHILDFDQDIYG*GIDVEI
IDFIRESIKVNNLDELKDLLNQDKAYAIKHFKGE
>Mature_273_residues
TIHYNEMLMML*NLPQTVAVFAEFDK*DENETNLIKKAKENNLKVILIQKEISNFKNIIPNKGIEQKAKDLDCELVL*HS
FNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIKYDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGFV
EHGNHIGRTINYPTANIIINDELILKEAVYLTTTKLPNDEKAYPSISAY*TNKNGVNVFESHILDFDQDIYG*GIDVEII
DFIRESIKVNNLDELKDLLNQDKAYAIKHFKGE

Specific function: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 [H]

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the mnmA/TRMU family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729
- InterPro:   IPR004506 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase; PF03054 tRNA_Me_trans [H]

EC number: =2.7.7.2; =2.7.1.26 [H]

Molecular weight: Translated: 31182; Mature: 31051

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHYNEMLMMLNLPQTVAVFAEFDKDENETNLIKKAKENNLKVILIQKEISNFKNIIPN
CEEEHHEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCC
KGIEQKAKDLDCELVLHSFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIK
CCHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCEEEE
YDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGFVEHGNHIGRTINYPTANIIINDE
ECHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEECCCCEEEEECCH
LILKEAVYLTTTKLPNDEKAYPSISAYTNKNGVNVFESHILDFDQDIYGGIDVEIIDFIR
HHHHHHEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHH
ESIKVNNLDELKDLLNQDKAYAIKHFKGE
HHHCCCCHHHHHHHHCCCHHEEHHCCCCC
>Mature Secondary Structure 
TIHYNEMLMMLNLPQTVAVFAEFDKDENETNLIKKAKENNLKVILIQKEISNFKNIIPN
EEEHHEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCC
KGIEQKAKDLDCELVLHSFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIK
CCHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCEEEE
YDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGFVEHGNHIGRTINYPTANIIINDE
ECHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEECCCCEEEEECCH
LILKEAVYLTTTKLPNDEKAYPSISAYTNKNGVNVFESHILDFDQDIYGGIDVEIIDFIR
HHHHHHEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHH
ESIKVNNLDELKDLLNQDKAYAIKHFKGE
HHHCCCCHHHHHHHHCCCHHEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949158 [H]