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The map label for this gene is gyrB [H]

Identifier: 50365125

GI number: 50365125

Start: 356072

End: 358015

Strand: Direct

Name: gyrB [H]

Synonym: Mfl309

Alternate gene names: 50365125

Gene position: 356072-358015 (Clockwise)

Preceding gene: 50365124

Following gene: 50365126

Centisome position: 44.89

GC content: 30.56

Gene sequence:

>1944_bases
ATGGCAAAAGAAGTAAAATATGATGAATCGGCCATTCAGGTCCTTGAAGGTTTAGAAGCTGTTAGAAAACGTCCAGGGAT
GTATATTGGTTCAACTGATGTTAGAGGACTACACCACTTAGTATGAGAAATTGTAGATAACTCAATCGATGAAGCTTTAG
CAGGATATTGTACAGAAATTAACGTAACTTTAGAAAAAAATGGAAGTGTTACTGTCGCTGATAATGGTCGTGGAGTTCCA
ATTGGGATGCACTCAACAGGAAGACCAACACCTGAAGTTATTTTTAGTGTACTTCATGCTGGAGGAAAATTTGGTGGAAG
TGGTTATAAAACATCTGGAGGACTTCACGGAGTTGGGTCATCTGTTGTTAATGCTTTATCTAAAAAATTTAATGTTACAA
TTTATCGTGATAAAAAAGTTAATGAAATTGAATTTACTAATGGAGGAAAATTAAATATTCCTTTAACAGAAGTTGGAACA
ACTAACAAAACTGGTACTGTTGTTAACTTCTTACCAGATGATTCAATTTTTAATACTACAAAATTTAACTTCACAACAAT
TAGTGAAAGATTAAAAGAATCAGCTTTACTAAACTCAGGTTTAAAAATTACAATATCAGACAAAGTTAATGATAAGTTTG
TTGAATATCAATTTGAAAATGGTTTAGTAGAGTTTGTTAAGGAATTATCAAGTGAATTTACTCATATTACTGATCCTGTT
GTTATTACTGGAGAATCAAAAAGAATTGCTTCAGAAATTTGTATTCAATATACTGAAGATTTTAATGAAACTATTTTAGG
TTTTGCTAATAATGTTAAAACTGGAGATGGTGGAACACACATTACTGGATTTAAAACTGGATTAGTTAGAGCAATTAATG
ATTATGCTAAAAATAATAAAATCTTAAAGGATAAAGATCCAAGATTAGATTCAAATGATTTAAGAGAAGGATTAGTAGCT
ATAGTTACTGTTAAAATTCCTGAAGACTTAATTGAGTATGAAGGGCAAACTAAAGGTAAGTTAGGAACACCAGATGCTAA
AACAGCTGTTGAACAAGTTACTTATGACTTTATGAACTTCTGATTAATTGAAAATAAAGTACCAGCTACAAAAATTATTG
AAAAAGCAATGTTGGCAAGAAGAGCTAGAGAAGAAGCTCGTAAAGCAAGACAAGCAATTCGTGATTCAAAAGGTAAAAAA
ACTACAAGAGCAATGCTAGGTAAATTAACTCCAGCTCAAGGAAGAAAAAAAGAAATTAATGAATTATATCTTGTTGAAGG
GGATTCAGCTGGAGGAAGTGCAAAATCAGGAAGAGATCGTACATTCCAAGCTATTCTACCTTTAAGAGGAAAAGTTATTA
ACTCTGAAAAAGCTAAATTAACAGAATTAATGAAAAATGAAGAAATTCAAACAATTATTACTGCAATTGGTGCTGGAATT
GGCCAAGACTTTGATGTTAGTGACATTAACTATGGAAAAGTAATTATTATGACCGATGCTGATACTGATGGGGCACATAT
TCAAACCTTATTATTAACATTCTTTTACAGGTATATGAAAGATTTGATTATTAATAAGCATGTTTTTATTGCATTACCAC
CACTATATAAATTAACTTTTTCAGATCGTAAATTTATTTATTTATGAGATGAACAAGAATTAGCTGATTTTGCTAAAACA
GCTACTAAAAAATATGAAATCCAACGTTATAAAGGACTTGGGGAAATGAATGCTGATCAATTATGAGAAACAACAATGAA
TCCAGCACAACGTAAATTAATTGTGGTAACTATTGAAGATGCATTAATGGCAGAAAAATCATTCAGAACTTTAATGGGTG
AAGATGCTGAAAAACGTAAAGTATGAATTCAAGAAAATGTTAAATTCACTTTAGAAGATAATGATGATGCAATCATCTTA
AATGAGAAACAAGAAAATAACTAG

Upstream 100 bases:

>100_bases
TATTTTTGTATGGGAAATGCTCTTGTGGTTCCATTAGTAGCAAAAATAGCTGATTGTTTAAGTGAAAAAAATAACGAAAT
AAAAAAATAAGGAGATTATT

Downstream 100 bases:

>100_bases
AAAGGAGAAATATACATGGCAAAAAAAATAGATAATTCAAATATTGAATCTGAAAAAGGAATAATTAGTTATGCTTTAGA
AGACTTAATGGGTGAACGAT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MAKEVKYDESAIQVLEGLEAVRKRPGMYIGSTDVRGLHHLVWEIVDNSIDEALAGYCTEINVTLEKNGSVTVADNGRGVP
IGMHSTGRPTPEVIFSVLHAGGKFGGSGYKTSGGLHGVGSSVVNALSKKFNVTIYRDKKVNEIEFTNGGKLNIPLTEVGT
TNKTGTVVNFLPDDSIFNTTKFNFTTISERLKESALLNSGLKITISDKVNDKFVEYQFENGLVEFVKELSSEFTHITDPV
VITGESKRIASEICIQYTEDFNETILGFANNVKTGDGGTHITGFKTGLVRAINDYAKNNKILKDKDPRLDSNDLREGLVA
IVTVKIPEDLIEYEGQTKGKLGTPDAKTAVEQVTYDFMNFWLIENKVPATKIIEKAMLARRAREEARKARQAIRDSKGKK
TTRAMLGKLTPAQGRKKEINELYLVEGDSAGGSAKSGRDRTFQAILPLRGKVINSEKAKLTELMKNEEIQTIITAIGAGI
GQDFDVSDINYGKVIIMTDADTDGAHIQTLLLTFFYRYMKDLIINKHVFIALPPLYKLTFSDRKFIYLWDEQELADFAKT
ATKKYEIQRYKGLGEMNADQLWETTMNPAQRKLIVVTIEDALMAEKSFRTLMGEDAEKRKVWIQENVKFTLEDNDDAIIL
NEKQENN

Sequences:

>Translated_647_residues
MAKEVKYDESAIQVLEGLEAVRKRPGMYIGSTDVRGLHHLV*EIVDNSIDEALAGYCTEINVTLEKNGSVTVADNGRGVP
IGMHSTGRPTPEVIFSVLHAGGKFGGSGYKTSGGLHGVGSSVVNALSKKFNVTIYRDKKVNEIEFTNGGKLNIPLTEVGT
TNKTGTVVNFLPDDSIFNTTKFNFTTISERLKESALLNSGLKITISDKVNDKFVEYQFENGLVEFVKELSSEFTHITDPV
VITGESKRIASEICIQYTEDFNETILGFANNVKTGDGGTHITGFKTGLVRAINDYAKNNKILKDKDPRLDSNDLREGLVA
IVTVKIPEDLIEYEGQTKGKLGTPDAKTAVEQVTYDFMNF*LIENKVPATKIIEKAMLARRAREEARKARQAIRDSKGKK
TTRAMLGKLTPAQGRKKEINELYLVEGDSAGGSAKSGRDRTFQAILPLRGKVINSEKAKLTELMKNEEIQTIITAIGAGI
GQDFDVSDINYGKVIIMTDADTDGAHIQTLLLTFFYRYMKDLIINKHVFIALPPLYKLTFSDRKFIYL*DEQELADFAKT
ATKKYEIQRYKGLGEMNADQL*ETTMNPAQRKLIVVTIEDALMAEKSFRTLMGEDAEKRKV*IQENVKFTLEDNDDAIIL
NEKQENN
>Mature_646_residues
AKEVKYDESAIQVLEGLEAVRKRPGMYIGSTDVRGLHHLV*EIVDNSIDEALAGYCTEINVTLEKNGSVTVADNGRGVPI
GMHSTGRPTPEVIFSVLHAGGKFGGSGYKTSGGLHGVGSSVVNALSKKFNVTIYRDKKVNEIEFTNGGKLNIPLTEVGTT
NKTGTVVNFLPDDSIFNTTKFNFTTISERLKESALLNSGLKITISDKVNDKFVEYQFENGLVEFVKELSSEFTHITDPVV
ITGESKRIASEICIQYTEDFNETILGFANNVKTGDGGTHITGFKTGLVRAINDYAKNNKILKDKDPRLDSNDLREGLVAI
VTVKIPEDLIEYEGQTKGKLGTPDAKTAVEQVTYDFMNF*LIENKVPATKIIEKAMLARRAREEARKARQAIRDSKGKKT
TRAMLGKLTPAQGRKKEINELYLVEGDSAGGSAKSGRDRTFQAILPLRGKVINSEKAKLTELMKNEEIQTIITAIGAGIG
QDFDVSDINYGKVIIMTDADTDGAHIQTLLLTFFYRYMKDLIINKHVFIALPPLYKLTFSDRKFIYL*DEQELADFAKTA
TKKYEIQRYKGLGEMNADQL*ETTMNPAQRKLIVVTIEDALMAEKSFRTLMGEDAEKRKV*IQENVKFTLEDNDDAIILN
EKQENN

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=613, Percent_Identity=24.6329526916803, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI19913408, Length=638, Percent_Identity=24.294670846395, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI48994957, Length=537, Percent_Identity=45.8100558659218, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1789408, Length=601, Percent_Identity=37.4376039933444, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI212645845, Length=561, Percent_Identity=25.4901960784314, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17535065, Length=163, Percent_Identity=30.0613496932515, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI212645657, Length=182, Percent_Identity=27.4725274725275, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=668, Percent_Identity=24.1017964071856, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136538, Length=673, Percent_Identity=23.7741456166419, Blast_Score=119, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71131; Mature: 71000

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEVKYDESAIQVLEGLEAVRKRPGMYIGSTDVRGLHHLVEIVDNSIDEALAGYCTEIN
CCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
VTLEKNGSVTVADNGRGVPIGMHSTGRPTPEVIFSVLHAGGKFGGSGYKTSGGLHGVGSS
EEEECCCCEEEECCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
VVNALSKKFNVTIYRDKKVNEIEFTNGGKLNIPLTEVGTTNKTGTVVNFLPDDSIFNTTK
HHHHHHHHCEEEEEECCCCCEEEECCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCE
FNFTTISERLKESALLNSGLKITISDKVNDKFVEYQFENGLVEFVKELSSEFTHITDPVV
EEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEE
ITGESKRIASEICIQYTEDFNETILGFANNVKTGDGGTHITGFKTGLVRAINDYAKNNKI
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCE
LKDKDPRLDSNDLREGLVAIVTVKIPEDLIEYEGQTKGKLGTPDAKTAVEQVTYDFMNFL
ECCCCCCCCHHHHHCCEEEEEEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IENKVPATKIIEKAMLARRAREEARKARQAIRDSKGKKTTRAMLGKLTPAQGRKKEINEL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHCCCEE
YLVEGDSAGGSAKSGRDRTFQAILPLRGKVINSEKAKLTELMKNEEIQTIITAIGAGIGQ
EEEECCCCCCCCCCCCCCCHHEECCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCC
DFDVSDINYGKVIIMTDADTDGAHIQTLLLTFFYRYMKDLIINKHVFIALPPLYKLTFSD
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECC
RKFIYLDEQELADFAKTATKKYEIQRYKGLGEMNADQLETTMNPAQRKLIVVTIEDALMA
CEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEECHHHHH
EKSFRTLMGEDAEKRKVIQENVKFTLEDNDDAIILNEKQENN
HHHHHHHHCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure 
AKEVKYDESAIQVLEGLEAVRKRPGMYIGSTDVRGLHHLVEIVDNSIDEALAGYCTEIN
CCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
VTLEKNGSVTVADNGRGVPIGMHSTGRPTPEVIFSVLHAGGKFGGSGYKTSGGLHGVGSS
EEEECCCCEEEECCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
VVNALSKKFNVTIYRDKKVNEIEFTNGGKLNIPLTEVGTTNKTGTVVNFLPDDSIFNTTK
HHHHHHHHCEEEEEECCCCCEEEECCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCE
FNFTTISERLKESALLNSGLKITISDKVNDKFVEYQFENGLVEFVKELSSEFTHITDPVV
EEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEE
ITGESKRIASEICIQYTEDFNETILGFANNVKTGDGGTHITGFKTGLVRAINDYAKNNKI
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCE
LKDKDPRLDSNDLREGLVAIVTVKIPEDLIEYEGQTKGKLGTPDAKTAVEQVTYDFMNFL
ECCCCCCCCHHHHHCCEEEEEEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IENKVPATKIIEKAMLARRAREEARKARQAIRDSKGKKTTRAMLGKLTPAQGRKKEINEL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHCCCEE
YLVEGDSAGGSAKSGRDRTFQAILPLRGKVINSEKAKLTELMKNEEIQTIITAIGAGIGQ
EEEECCCCCCCCCCCCCCCHHEECCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCC
DFDVSDINYGKVIIMTDADTDGAHIQTLLLTFFYRYMKDLIINKHVFIALPPLYKLTFSD
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECC
RKFIYLDEQELADFAKTATKKYEIQRYKGLGEMNADQLETTMNPAQRKLIVVTIEDALMA
CEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEECHHHHH
EKSFRTLMGEDAEKRKVIQENVKFTLEDNDDAIILNEKQENN
HHHHHHHHCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7828871 [H]