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The map label for this gene is sau3AIM [H]

Identifier: 50365124

GI number: 50365124

Start: 354847

End: 356061

Strand: Direct

Name: sau3AIM [H]

Synonym: Mfl308

Alternate gene names: 50365124

Gene position: 354847-356061 (Clockwise)

Preceding gene: 50365122

Following gene: 50365125

Centisome position: 44.73

GC content: 26.58

Gene sequence:

>1215_bases
ATGTCTGATAAAATCAAAGTCGTTGAATTATTTGCAGGAGTAGGTGGATTTTCTCTTGCATTGAAGAAATCAAAAGGTAA
TTATGAAGTTATTTTTTCAAACCAGTGAGAACCTTCAACTAAAAATCAATTTGCTTTTAATGCACTTAATAAAAATTTTA
AAAAGCACATTTTATCTAATGAAGATATTCAGTTTGCAAAAGAAAAATTACCAAACGATTTTGATTTACTTGTTGGAGGA
TTTCCTTGTCAAGATTATTCGGTAGCAACTACAAACTCAAAAGGTATTGAAGGAAAAAAAGGTGTGCTTTGATGAGAAAT
TTCTTGAATTTTAGAAAAGCATAAACCAAATTTTGTTTTTCTTGAAAACGTGGATAGATTATTAAAGTCTCCCTCAAATA
AAAGAGGAAGAGACTTTGCAATAATACTTAAAGATCTTGACGAAAAAGGTTATAACGTCGAGTGAATGAATATAAATGCT
GGTGATTATGGCTATGTTCAAAGAAGAAGAAGAGTATTTATTCTTGCTTGAAGAAAAGAAAAATTAAATATTCAGAAGGA
ATTAAATATTTCAGATGCATTTCATAAAGGAATATTCAGAAAAGGTTTTAAAGTTAAAAAGTTTATATTAGAAAAAGAAA
TTGACTTAAATATTTTTTCAGATAAAGTTGATGTTACTGAAAATTATAATTATGGAAAATTTTTAAATTTTGGAGTAATG
ATAGACGGAAAAATAATTTCAGGAGATTTCGTTCCACTTTTTAAAGGAAAACAAAAAAAATTAAAAGATATATTAGAAGA
TAAAGTTGATGATTCATATTTTTTAACTGAAGAACAAATACAAAAAATAAGTATTTTAAAAGATAGCAAAAAAATTCCTA
GAACAAAACCTAATGGTGAAAAATATTTTTACTCTGAAGGTAAGATGTCAATACTTGAAAATATAAATATGCCATCAAGA
ACAATGTTGACATCTGAAGGAACAATAAATAGAGCATCGCATTTTATTGAAGATACTAATTCAAAAGGAATTAAAGGTAT
TAGAAGGATAACACCATTAGAAGCTGAAAGAATAAATGGTTTTACCGATAATTGGACTTCTGACGTAATGACAGAAAGAC
AAAGATATTTTTGTATGGGAAATGCTCTTGTGGTTCCATTAGTAGCAAAAATAGCTGATTGTTTAAGTGAAAAAAATAAC
GAAATAAAAAAATAA

Upstream 100 bases:

>100_bases
ATATCATCTATTGTCTTATTCATATTATATTTTCTCTTCCTATTTTAGTGTATAATTTATTAATGTTATTATAAAATATT
TTATGAAAGAGTGTGAAATA

Downstream 100 bases:

>100_bases
GGAGATTATTATGGCAAAAGAAGTAAAATATGATGAATCGGCCATTCAGGTCCTTGAAGGTTTAGAAGCTGTTAGAAAAC
GTCCAGGGATGTATATTGGT

Product: modification methylase Sau3AI (GATC cytosine-specific methyltransferase)

Products: NA

Alternate protein names: M.Sau3AI; Cytosine-specific methyltransferase Sau3AI [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSDKIKVVELFAGVGGFSLALKKSKGNYEVIFSNQWEPSTKNQFAFNALNKNFKKHILSNEDIQFAKEKLPNDFDLLVGG
FPCQDYSVATTNSKGIEGKKGVLWWEISWILEKHKPNFVFLENVDRLLKSPSNKRGRDFAIILKDLDEKGYNVEWMNINA
GDYGYVQRRRRVFILAWRKEKLNIQKELNISDAFHKGIFRKGFKVKKFILEKEIDLNIFSDKVDVTENYNYGKFLNFGVM
IDGKIISGDFVPLFKGKQKKLKDILEDKVDDSYFLTEEQIQKISILKDSKKIPRTKPNGEKYFYSEGKMSILENINMPSR
TMLTSEGTINRASHFIEDTNSKGIKGIRRITPLEAERINGFTDNWTSDVMTERQRYFCMGNALVVPLVAKIADCLSEKNN
EIKK

Sequences:

>Translated_404_residues
MSDKIKVVELFAGVGGFSLALKKSKGNYEVIFSNQ*EPSTKNQFAFNALNKNFKKHILSNEDIQFAKEKLPNDFDLLVGG
FPCQDYSVATTNSKGIEGKKGVL**EIS*ILEKHKPNFVFLENVDRLLKSPSNKRGRDFAIILKDLDEKGYNVE*MNINA
GDYGYVQRRRRVFILA*RKEKLNIQKELNISDAFHKGIFRKGFKVKKFILEKEIDLNIFSDKVDVTENYNYGKFLNFGVM
IDGKIISGDFVPLFKGKQKKLKDILEDKVDDSYFLTEEQIQKISILKDSKKIPRTKPNGEKYFYSEGKMSILENINMPSR
TMLTSEGTINRASHFIEDTNSKGIKGIRRITPLEAERINGFTDNWTSDVMTERQRYFCMGNALVVPLVAKIADCLSEKNN
EIKK
>Mature_403_residues
SDKIKVVELFAGVGGFSLALKKSKGNYEVIFSNQ*EPSTKNQFAFNALNKNFKKHILSNEDIQFAKEKLPNDFDLLVGGF
PCQDYSVATTNSKGIEGKKGVL**EIS*ILEKHKPNFVFLENVDRLLKSPSNKRGRDFAIILKDLDEKGYNVE*MNINAG
DYGYVQRRRRVFILA*RKEKLNIQKELNISDAFHKGIFRKGFKVKKFILEKEIDLNIFSDKVDVTENYNYGKFLNFGVMI
DGKIISGDFVPLFKGKQKKLKDILEDKVDDSYFLTEEQIQKISILKDSKKIPRTKPNGEKYFYSEGKMSILENINMPSRT
MLTSEGTINRASHFIEDTNSKGIKGIRRITPLEAERINGFTDNWTSDVMTERQRYFCMGNALVVPLVAKIADCLSEKNNE
IKK

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on C-4 on both strands and protects the DNA from cleavage by the Sau3AI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=217, Percent_Identity=24.4239631336406, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 45435; Mature: 45304

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKIKVVELFAGVGGFSLALKKSKGNYEVIFSNQEPSTKNQFAFNALNKNFKKHILSNE
CCCCEEEEEEHHCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
DIQFAKEKLPNDFDLLVGGFPCQDYSVATTNSKGIEGKKGVLEISILEKHKPNFVFLENV
CHHHHHHHCCCCHHEEECCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCEEEHHHH
DRLLKSPSNKRGRDFAIILKDLDEKGYNVEMNINAGDYGYVQRRRRVFILARKEKLNIQK
HHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHCCCEEEEEEECCCCCCHH
ELNISDAFHKGIFRKGFKVKKFILEKEIDLNIFSDKVDVTENYNYGKFLNFGVMIDGKII
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEEEECEEE
SGDFVPLFKGKQKKLKDILEDKVDDSYFLTEEQIQKISILKDSKKIPRTKPNGEKYFYSE
CCCCEECCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
GKMSILENINMPSRTMLTSEGTINRASHFIEDTNSKGIKGIRRITPLEAERINGFTDNWT
CCHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHCCCCCCCCH
SDVMTERQRYFCMGNALVVPLVAKIADCLSEKNNEIKK
HHHHHHHHHEEEECCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDKIKVVELFAGVGGFSLALKKSKGNYEVIFSNQEPSTKNQFAFNALNKNFKKHILSNE
CCCEEEEEEHHCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
DIQFAKEKLPNDFDLLVGGFPCQDYSVATTNSKGIEGKKGVLEISILEKHKPNFVFLENV
CHHHHHHHCCCCHHEEECCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCEEEHHHH
DRLLKSPSNKRGRDFAIILKDLDEKGYNVEMNINAGDYGYVQRRRRVFILARKEKLNIQK
HHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHCCCEEEEEEECCCCCCHH
ELNISDAFHKGIFRKGFKVKKFILEKEIDLNIFSDKVDVTENYNYGKFLNFGVMIDGKII
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEEEECEEE
SGDFVPLFKGKQKKLKDILEDKVDDSYFLTEEQIQKISILKDSKKIPRTKPNGEKYFYSE
CCCCEECCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
GKMSILENINMPSRTMLTSEGTINRASHFIEDTNSKGIKGIRRITPLEAERINGFTDNWT
CCHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHCCCCCCCCH
SDVMTERQRYFCMGNALVVPLVAKIADCLSEKNNEIKK
HHHHHHHHHEEEECCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2227451 [H]