Click here to switch to the map view.

The map label for this gene is guaA [H]

Identifier: 50365093

GI number: 50365093

Start: 320350

End: 320913

Strand: Direct

Name: guaA [H]

Synonym: Mfl277

Alternate gene names: 50365093

Gene position: 320350-320913 (Clockwise)

Preceding gene: 50365092

Following gene: 50365094

Centisome position: 40.39

GC content: 21.63

Gene sequence:

>564_bases
ATGGAAGACAAACAAAGATGTGATTGATCATCAAATGCTATTTTAAATGAATATCATGATAATGAGTGAAGTAAAATAAC
TCAAAATGATGATTACATATTTGAATTACTTATATTAGAAAATATGCAAGCGGGTTTAAATTGATTAACAATCTTACTTA
AAAGAGAAGAATTCAAAAAAGCATTTGATAATTTTGAATTAAAAAAAATTATTAGCTATGATGAAAATAAAATAAATGAA
TTAATGAATAATAAGGGTATTATAAGAAATAAACTAAAAATTAAATCACTAATTTCAAACGCAAAAGCTTTTAAAGAAAT
TCAAATACAATTTGGTTCTTTTTATAATTACATTTGAACTTTTACAAATAACAAGCAAGTTATTAATAATTGAAATTCAA
TTGATGAAGTACCAGCTGAATCAGATTTATCAAAAACAATATCAGTGGATTTAAGAAAAAGAGGATTTAAATTTGTAGGA
CCTGTTATTGTTTATTCATTTTTACAAGCAATAGGAATAATCGATGATCATTTAAATAAATGTTTTTGTAAAAATCAAAA
TTAA

Upstream 100 bases:

>100_bases
TCTATTACAAGAATACAAAGTTCAAAAACTTGTTAAATATTAAGATTAACACCTATAATTAAAATAGGTGTTTTTTTATT
TAAAACAGAAAGGTATATTT

Downstream 100 bases:

>100_bases
TTATATAAGAGTGTATAGACTTATTTTAAGTCTATTTTTTGTTATAATTTTAAAAATAGAATGGGTGATTTCATGGCTGG
TATAATTTTACCTAGAAGAA

Product: DNA-3-methyladenine glycosidase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MEDKQRCDWSSNAILNEYHDNEWSKITQNDDYIFELLILENMQAGLNWLTILLKREEFKKAFDNFELKKIISYDENKINE
LMNNKGIIRNKLKIKSLISNAKAFKEIQIQFGSFYNYIWTFTNNKQVINNWNSIDEVPAESDLSKTISVDLRKRGFKFVG
PVIVYSFLQAIGIIDDHLNKCFCKNQN

Sequences:

>Translated_187_residues
MEDKQRCD*SSNAILNEYHDNE*SKITQNDDYIFELLILENMQAGLN*LTILLKREEFKKAFDNFELKKIISYDENKINE
LMNNKGIIRNKLKIKSLISNAKAFKEIQIQFGSFYNYI*TFTNNKQVINN*NSIDEVPAESDLSKTISVDLRKRGFKFVG
PVIVYSFLQAIGIIDDHLNKCFCKNQN
>Mature_187_residues
MEDKQRCD*SSNAILNEYHDNE*SKITQNDDYIFELLILENMQAGLN*LTILLKREEFKKAFDNFELKKIISYDENKINE
LMNNKGIIRNKLKIKSLISNAKAFKEIQIQFGSFYNYI*TFTNNKQVINN*NSIDEVPAESDLSKTISVDLRKRGFKFVG
PVIVYSFLQAIGIIDDHLNKCFCKNQN

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 N-acetyltransferase domains [H]

Homologues:

Organism=Escherichia coli, GI1789971, Length=184, Percent_Identity=36.4130434782609, Blast_Score=136, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR005019
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011257
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF03352 Adenine_glyco; PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 21069; Mature: 21069

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKQRCDSSNAILNEYHDNESKITQNDDYIFELLILENMQAGLNLTILLKREEFKKAFD
CCCHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHC
NFELKKIISYDENKINELMNNKGIIRNKLKIKSLISNAKAFKEIQIQFGSFYNYITFTNN
CCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECC
KQVINNNSIDEVPAESDLSKTISVDLRKRGFKFVGPVIVYSFLQAIGIIDDHLNKCFCKN
CEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QN
CC
>Mature Secondary Structure
MEDKQRCDSSNAILNEYHDNESKITQNDDYIFELLILENMQAGLNLTILLKREEFKKAFD
CCCHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHC
NFELKKIISYDENKINELMNNKGIIRNKLKIKSLISNAKAFKEIQIQFGSFYNYITFTNN
CCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECC
KQVINNNSIDEVPAESDLSKTISVDLRKRGFKFVGPVIVYSFLQAIGIIDDHLNKCFCKN
CEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]