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The map label for this gene is spoT [H]

Identifier: 50365094

GI number: 50365094

Start: 320986

End: 323283

Strand: Direct

Name: spoT [H]

Synonym: Mfl278

Alternate gene names: 50365094

Gene position: 320986-323283 (Clockwise)

Preceding gene: 50365093

Following gene: 50365095

Centisome position: 40.47

GC content: 27.02

Gene sequence:

>2298_bases
ATGGCTGGTATAATTTTACCTAGAAGAACTAGTAAAAGAGCTTCAACAAACTTAAATATTGTTGAGATAAAAGACTATAT
AACACTAGAAAATGAATTAAAAAAATATATTAAAGATAGAAAAGCATTAATTAAAATTAAGGAAGCTTATTTATTTGCTG
AAAAAATGCATAGCGAACAAAAACGTAAAAATGGAGATCCCTATATTTATCACCCACTTTCAACTGCATACTATTTAGCA
CAACTGCAAATGGGACCAAAAACTATTAAAGCTGGTTTATTACATGATGTTGTTGAAGATACACCTGTTAAAATTGAAGA
GATAGAAGAGAAATTTGGAAAAGAAATAGCATCACTTGTTGAATCTGTAACTAAAGTTAGTTATTTTGCCGAAGAAAATC
GTGAGCAAATTAAATCAGAATATTTAAGAAAACTGTATATTTCTATGGCAAAAGATATAAGGGTTATTATTATTAAAATT
GCTGATAGATTACATAATATGTTAACAATTGAGAATTTACCAGAAGAAAAACAAAGAGTAGTTGCTAAAGAGACATTAAC
TATTTATGCAGCCATTGCTCACCGTATAGGAATGAAAAATGCTAAATCTAAATTAGAAGACATGGCATTTAAAGTTTTAA
ATCCAAATGATTTTACAGACATTGAAAATTTAATTGAAAAAGGTCGTGAATCTAGGGAAGATAATATTGCTAAAACAATT
GATGATATTTCGTTTTTCTTAAGAAAAGAAAAACACATTAAAATAATTGATATTTTTGGAAGAGAAAAAACAATTTATTC
AATTTACAGAAAAATGAATGTTAATGGTAAACAATTTGAAGAATTACATGACCTTGTTGCAATTAGGATAATTGCAAAAT
CAACTGATGATTGCTACAAAATTTTAGGGTACTTACACCAAAAATACTTACCATTATCAGGAAGATTTAAAGATTATATT
GCAACACCTAAAAATAACGTATATCAGTCATTGCATACTACTTTAAGTAATAGTAAAGGAATGTTTTTTGAAGTTCAAAT
AAGAACTCAACAAATGGATGATGTCGCTGAAACTGGGGCTGCTGCTCACTGAAGATATAAAGAGGGTGAAGTTGTAGATA
TTGCTAAGAGACAAAAACAAATTGACGAACAAATTGATATATTTAGCAGAATTTTGGATTTAACAGATCAAATAAATGAA
GAAGAAAATTCAAAAGAGAAAGAATTAGAAAATCAATTACAAAAAGATGTTTTTGGATCAATGATTTATGTTCTAACTCC
AACTCAAAATGTTATTACTTTGCCTTATGGAGCAACTGTATTAGATTTTGCTTACAGAATACACACTGAAATAGGAGAAA
AAACTACAGGAGCAAAAATTGATGGTATATTCTCTCCAATTAACACAGTCTTAGAATCAGGGCAAGTTGTTGAAATTAAA
ACTTCTTCTAAACAACAACCAACACACGAATGATTAAAAATAGCTACTACATCAAATGCTAAAAATAGAATTAGAAAATA
CTTGGGTAATAAACTAAAAGAAGATAACTCATTTGATGAAGATAGAAAAGAATTAGCAAGAAAAACAGAAAACTTAATTA
ACTCATATATTAATCAAAAAGAAATAAAATGAAAAAGAAAATCTCAAAGTGAAATTCTTGAAGCTGTTAAAAAAGCAGGT
TATGCCAATCTTGAAGAGTTTTTAATTAATGTTGGTAAAGGTGAAATCTCTATTGTTGAAGCCACTGATAAATTCTTCAT
TAATCACAATTTCTCAAAGGACGAAGAAGCTTTAAGAAGTATTCAATCAAAAACTATAAATGATAGAAGTTTAAAAAATG
ATATTGTTATAGATGGAATAACAAATATTAAAACATCAATTGCAAGTTGTTGTTTACCAATTCCATATGAAGATGTTATT
GGTTATGTTGCTAAATCAGGAAATGGTATAAAAGTTCATTTAAAAGAATGTTATAACCTATATACAACAGAAGAAACAAA
ACGTTTAGTTCAAGTACAATGAAACTCAGCTGTTGCTGAAAATAGTTTATATACAACAAAACTAAAATATTTTGCTACAG
ATAGACCAAATCTTTTATATGACATATCAAGAGCATTATCAAATTTAAAAGCAACAACAATAAATGTAAAACTTGGTGTT
GATGATAAATCTTTACTTGTTAATGGTGAATTAACAATTAAAGTTAAAAGTTCAGCACAATTAAATCAAATTATTTTAAC
AATTAAATCAATTGCAAATATCATAGATTGCGAAAGATCTGTAAAGAGTATTAAATAG

Upstream 100 bases:

>100_bases
TAAATGTTTTTGTAAAAATCAAAATTAATTATATAAGAGTGTATAGACTTATTTTAAGTCTATTTTTTGTTATAATTTTA
AAAATAGAATGGGTGATTTC

Downstream 100 bases:

>100_bases
GTTTTAAACCTATTTTTTATTTTTCTAAATTTTTTCCAATATTTTTTATTAATAATATAATGACATTGAAAGGAACTAAA
AATCGATGAAAAAAAGAAAA

Product: guanosine-3', 5'-bis(diphosphate)3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MAGIILPRRTSKRASTNLNIVEIKDYITLENELKKYIKDRKALIKIKEAYLFAEKMHSEQKRKNGDPYIYHPLSTAYYLA
QLQMGPKTIKAGLLHDVVEDTPVKIEEIEEKFGKEIASLVESVTKVSYFAEENREQIKSEYLRKLYISMAKDIRVIIIKI
ADRLHNMLTIENLPEEKQRVVAKETLTIYAAIAHRIGMKNAKSKLEDMAFKVLNPNDFTDIENLIEKGRESREDNIAKTI
DDISFFLRKEKHIKIIDIFGREKTIYSIYRKMNVNGKQFEELHDLVAIRIIAKSTDDCYKILGYLHQKYLPLSGRFKDYI
ATPKNNVYQSLHTTLSNSKGMFFEVQIRTQQMDDVAETGAAAHWRYKEGEVVDIAKRQKQIDEQIDIFSRILDLTDQINE
EENSKEKELENQLQKDVFGSMIYVLTPTQNVITLPYGATVLDFAYRIHTEIGEKTTGAKIDGIFSPINTVLESGQVVEIK
TSSKQQPTHEWLKIATTSNAKNRIRKYLGNKLKEDNSFDEDRKELARKTENLINSYINQKEIKWKRKSQSEILEAVKKAG
YANLEEFLINVGKGEISIVEATDKFFINHNFSKDEEALRSIQSKTINDRSLKNDIVIDGITNIKTSIASCCLPIPYEDVI
GYVAKSGNGIKVHLKECYNLYTTEETKRLVQVQWNSAVAENSLYTTKLKYFATDRPNLLYDISRALSNLKATTINVKLGV
DDKSLLVNGELTIKVKSSAQLNQIILTIKSIANIIDCERSVKSIK

Sequences:

>Translated_765_residues
MAGIILPRRTSKRASTNLNIVEIKDYITLENELKKYIKDRKALIKIKEAYLFAEKMHSEQKRKNGDPYIYHPLSTAYYLA
QLQMGPKTIKAGLLHDVVEDTPVKIEEIEEKFGKEIASLVESVTKVSYFAEENREQIKSEYLRKLYISMAKDIRVIIIKI
ADRLHNMLTIENLPEEKQRVVAKETLTIYAAIAHRIGMKNAKSKLEDMAFKVLNPNDFTDIENLIEKGRESREDNIAKTI
DDISFFLRKEKHIKIIDIFGREKTIYSIYRKMNVNGKQFEELHDLVAIRIIAKSTDDCYKILGYLHQKYLPLSGRFKDYI
ATPKNNVYQSLHTTLSNSKGMFFEVQIRTQQMDDVAETGAAAH*RYKEGEVVDIAKRQKQIDEQIDIFSRILDLTDQINE
EENSKEKELENQLQKDVFGSMIYVLTPTQNVITLPYGATVLDFAYRIHTEIGEKTTGAKIDGIFSPINTVLESGQVVEIK
TSSKQQPTHE*LKIATTSNAKNRIRKYLGNKLKEDNSFDEDRKELARKTENLINSYINQKEIK*KRKSQSEILEAVKKAG
YANLEEFLINVGKGEISIVEATDKFFINHNFSKDEEALRSIQSKTINDRSLKNDIVIDGITNIKTSIASCCLPIPYEDVI
GYVAKSGNGIKVHLKECYNLYTTEETKRLVQVQ*NSAVAENSLYTTKLKYFATDRPNLLYDISRALSNLKATTINVKLGV
DDKSLLVNGELTIKVKSSAQLNQIILTIKSIANIIDCERSVKSIK
>Mature_764_residues
AGIILPRRTSKRASTNLNIVEIKDYITLENELKKYIKDRKALIKIKEAYLFAEKMHSEQKRKNGDPYIYHPLSTAYYLAQ
LQMGPKTIKAGLLHDVVEDTPVKIEEIEEKFGKEIASLVESVTKVSYFAEENREQIKSEYLRKLYISMAKDIRVIIIKIA
DRLHNMLTIENLPEEKQRVVAKETLTIYAAIAHRIGMKNAKSKLEDMAFKVLNPNDFTDIENLIEKGRESREDNIAKTID
DISFFLRKEKHIKIIDIFGREKTIYSIYRKMNVNGKQFEELHDLVAIRIIAKSTDDCYKILGYLHQKYLPLSGRFKDYIA
TPKNNVYQSLHTTLSNSKGMFFEVQIRTQQMDDVAETGAAAH*RYKEGEVVDIAKRQKQIDEQIDIFSRILDLTDQINEE
ENSKEKELENQLQKDVFGSMIYVLTPTQNVITLPYGATVLDFAYRIHTEIGEKTTGAKIDGIFSPINTVLESGQVVEIKT
SSKQQPTHE*LKIATTSNAKNRIRKYLGNKLKEDNSFDEDRKELARKTENLINSYINQKEIK*KRKSQSEILEAVKKAGY
ANLEEFLINVGKGEISIVEATDKFFINHNFSKDEEALRSIQSKTINDRSLKNDIVIDGITNIKTSIASCCLPIPYEDVIG
YVAKSGNGIKVHLKECYNLYTTEETKRLVQVQ*NSAVAENSLYTTKLKYFATDRPNLLYDISRALSNLKATTINVKLGVD
DKSLLVNGELTIKVKSSAQLNQIILTIKSIANIIDCERSVKSIK

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=724, Percent_Identity=33.8397790055249, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1789147, Length=731, Percent_Identity=28.8645690834473, Blast_Score=333, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25143118, Length=123, Percent_Identity=36.5853658536585, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86918; Mature: 86787

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIILPRRTSKRASTNLNIVEIKDYITLENELKKYIKDRKALIKIKEAYLFAEKMHSEQ
CCCCCCCCCCCCHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRKNGDPYIYHPLSTAYYLAQLQMGPKTIKAGLLHDVVEDTPVKIEEIEEKFGKEIASLV
HHCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ESVTKVSYFAEENREQIKSEYLRKLYISMAKDIRVIIIKIADRLHNMLTIENLPEEKQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VAKETLTIYAAIAHRIGMKNAKSKLEDMAFKVLNPNDFTDIENLIEKGRESREDNIAKTI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
DDISFFLRKEKHIKIIDIFGREKTIYSIYRKMNVNGKQFEELHDLVAIRIIAKSTDDCYK
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
ILGYLHQKYLPLSGRFKDYIATPKNNVYQSLHTTLSNSKGMFFEVQIRTQQMDDVAETGA
HHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHCH
AAHRYKEGEVVDIAKRQKQIDEQIDIFSRILDLTDQINEEENSKEKELENQLQKDVFGSM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCE
IYVLTPTQNVITLPYGATVLDFAYRIHTEIGEKTTGAKIDGIFSPINTVLESGQVVEIKT
EEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
SSKQQPTHELKIATTSNAKNRIRKYLGNKLKEDNSFDEDRKELARKTENLINSYINQKEI
CCCCCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
KKRKSQSEILEAVKKAGYANLEEFLINVGKGEISIVEATDKFFINHNFSKDEEALRSIQS
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
KTINDRSLKNDIVIDGITNIKTSIASCCLPIPYEDVIGYVAKSGNGIKVHLKECYNLYTT
HCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHCC
EETKRLVQVQNSAVAENSLYTTKLKYFATDRPNLLYDISRALSNLKATTINVKLGVDDKS
HHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEECCCCCC
LLVNGELTIKVKSSAQLNQIILTIKSIANIIDCERSVKSIK
EEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGIILPRRTSKRASTNLNIVEIKDYITLENELKKYIKDRKALIKIKEAYLFAEKMHSEQ
CCCCCCCCCCCHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRKNGDPYIYHPLSTAYYLAQLQMGPKTIKAGLLHDVVEDTPVKIEEIEEKFGKEIASLV
HHCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ESVTKVSYFAEENREQIKSEYLRKLYISMAKDIRVIIIKIADRLHNMLTIENLPEEKQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VAKETLTIYAAIAHRIGMKNAKSKLEDMAFKVLNPNDFTDIENLIEKGRESREDNIAKTI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
DDISFFLRKEKHIKIIDIFGREKTIYSIYRKMNVNGKQFEELHDLVAIRIIAKSTDDCYK
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
ILGYLHQKYLPLSGRFKDYIATPKNNVYQSLHTTLSNSKGMFFEVQIRTQQMDDVAETGA
HHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHCH
AAHRYKEGEVVDIAKRQKQIDEQIDIFSRILDLTDQINEEENSKEKELENQLQKDVFGSM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCE
IYVLTPTQNVITLPYGATVLDFAYRIHTEIGEKTTGAKIDGIFSPINTVLESGQVVEIKT
EEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
SSKQQPTHELKIATTSNAKNRIRKYLGNKLKEDNSFDEDRKELARKTENLINSYINQKEI
CCCCCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
KKRKSQSEILEAVKKAGYANLEEFLINVGKGEISIVEATDKFFINHNFSKDEEALRSIQS
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
KTINDRSLKNDIVIDGITNIKTSIASCCLPIPYEDVIGYVAKSGNGIKVHLKECYNLYTT
HCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHCC
EETKRLVQVQNSAVAENSLYTTKLKYFATDRPNLLYDISRALSNLKATTINVKLGVDDKS
HHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEECCCCCC
LLVNGELTIKVKSSAQLNQIILTIKSIANIIDCERSVKSIK
EEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9244268 [H]