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The map label for this gene is era [H]

Identifier: 50365082

GI number: 50365082

Start: 304751

End: 305656

Strand: Direct

Name: era [H]

Synonym: Mfl266

Alternate gene names: 50365082

Gene position: 304751-305656 (Clockwise)

Preceding gene: 50365081

Following gene: 50365083

Centisome position: 38.42

GC content: 25.72

Gene sequence:

>906_bases
ATGAATAAAATTAAATCGGGATTTATATCTATAGTTGGTAGACCAAACGTTGGTAAATCAACTTTATTAAATAAAATAAT
AGGGCATAAAATTTCAATTGTTACAAATAAAGCTCAAACAACAAGAAATAATATAAGAGGTATTTTGACAGAAAAAGAAT
ATCAATTGATATTTGTAGATACACCAGGAATACACACTTCAAAAAATCAAATTGATAGATTTATGAATTCTAGTGCTATG
AGAAGTATGAAAGAAGTTGATGTTGTTGTATTTATGGCACCAGCTGATGAAACAATAGGAAAAAATGATTTATTTATATT
AAATGAATTATCAAAAAAGAATGACATTAAAAAGATACTTGTAATTTCAAAAGCTGACGTTGTTAGCAAAGAAAAATTAT
TTTTAAAAGCTACTGAATGAAATACATATGAACAAATTTTTGATGAAATTATTATTACTTCGTCAACTGAAAATATTAAT
ATTGATAAATTAATAGAAACAATTGTTGGTTTCTTGCCTGAAACAGGTCATTACTTTTATGATGAAGAATCTATTACAGA
TCAACCTAATCGTTTTGCAATAAGAGAAATAATAAGAGAAAGCGTACTTTTAAAAGCTGGACAAGAAGTTCCTCATTCAG
TTGCTATACTTGTAGATGAACTTGAAGAAACAGAAGATGAAATTAATATTGTTGCATCTATTATAGTTGAAAGAAAATCT
CAAAAAGGAATTATTATCGGTCATCAAGGTAAAAAAATAAGCGATATTAAATATAAATCAAGAAAACAAATAAGAGAACT
TTTTGAAAAAGATGTTAATCTAGAATTATTTGTAAAAGTCCAAGAGAATTGAAGAAACTCTGCAAGTTTAATTAAAAAGA
TGGGATACGACAAGGATAAATACTAA

Upstream 100 bases:

>100_bases
GAAGAAATGTTTTTCATTCAAGATGAAATACTTAAAATAAATAAAATAAATTACACAATAAAATTCACAGAAGAAGATTA
TAAAGAAATAGAGGAAAAAG

Downstream 100 bases:

>100_bases
TGAGTGAAGTAAAAATCAGAGGAATAGTTTTAGATTCTTTAAATTATGAAGAAAATGACAAAATTATTACAGTTTATTCT
GATGAATTTGGGAAACTAAG

Product: GTP-binding protein Era

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNKIKSGFISIVGRPNVGKSTLLNKIIGHKISIVTNKAQTTRNNIRGILTEKEYQLIFVDTPGIHTSKNQIDRFMNSSAM
RSMKEVDVVVFMAPADETIGKNDLFILNELSKKNDIKKILVISKADVVSKEKLFLKATEWNTYEQIFDEIIITSSTENIN
IDKLIETIVGFLPETGHYFYDEESITDQPNRFAIREIIRESVLLKAGQEVPHSVAILVDELEETEDEINIVASIIVERKS
QKGIIIGHQGKKISDIKYKSRKQIRELFEKDVNLELFVKVQENWRNSASLIKKMGYDKDKY

Sequences:

>Translated_301_residues
MNKIKSGFISIVGRPNVGKSTLLNKIIGHKISIVTNKAQTTRNNIRGILTEKEYQLIFVDTPGIHTSKNQIDRFMNSSAM
RSMKEVDVVVFMAPADETIGKNDLFILNELSKKNDIKKILVISKADVVSKEKLFLKATE*NTYEQIFDEIIITSSTENIN
IDKLIETIVGFLPETGHYFYDEESITDQPNRFAIREIIRESVLLKAGQEVPHSVAILVDELEETEDEINIVASIIVERKS
QKGIIIGHQGKKISDIKYKSRKQIRELFEKDVNLELFVKVQEN*RNSASLIKKMGYDKDKY
>Mature_301_residues
MNKIKSGFISIVGRPNVGKSTLLNKIIGHKISIVTNKAQTTRNNIRGILTEKEYQLIFVDTPGIHTSKNQIDRFMNSSAM
RSMKEVDVVVFMAPADETIGKNDLFILNELSKKNDIKKILVISKADVVSKEKLFLKATE*NTYEQIFDEIIITSSTENIN
IDKLIETIVGFLPETGHYFYDEESITDQPNRFAIREIIRESVLLKAGQEVPHSVAILVDELEETEDEINIVASIIVERKS
QKGIIIGHQGKKISDIKYKSRKQIRELFEKDVNLELFVKVQEN*RNSASLIKKMGYDKDKY

Specific function: An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism [H]

COG id: COG1159

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI24307899, Length=321, Percent_Identity=26.4797507788162, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1788919, Length=301, Percent_Identity=39.8671096345515, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI87082120, Length=130, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24646434, Length=310, Percent_Identity=22.5806451612903, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005662
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004044
- InterPro:   IPR002917
- InterPro:   IPR005225 [H]

Pfam domain/function: PF07650 KH_2; PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 34041; Mature: 34041

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50823 KH_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIKSGFISIVGRPNVGKSTLLNKIIGHKISIVTNKAQTTRNNIRGILTEKEYQLIFVD
CCCHHCCCEEEEECCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHCEEEECCCEEEEEEE
TPGIHTSKNQIDRFMNSSAMRSMKEVDVVVFMAPADETIGKNDLFILNELSKKNDIKKIL
CCCCCCCHHHHHHHHCHHHHHHHHHCEEEEEEECCCCCCCCCCEEEEECHHCCCCCEEEE
VISKADVVSKEKLFLKATENTYEQIFDEIIITSSTENINIDKLIETIVGFLPETGHYFYD
EEECCCCCCCCCEEEEECHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCEEEC
EESITDQPNRFAIREIIRESVLLKAGQEVPHSVAILVDELEETEDEINIVASIIVERKSQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGIIIGHQGKKISDIKYKSRKQIRELFEKDVNLELFVKVQENRNSASLIKKMGYDKDKY
CCEEEECCCCEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKIKSGFISIVGRPNVGKSTLLNKIIGHKISIVTNKAQTTRNNIRGILTEKEYQLIFVD
CCCHHCCCEEEEECCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHCEEEECCCEEEEEEE
TPGIHTSKNQIDRFMNSSAMRSMKEVDVVVFMAPADETIGKNDLFILNELSKKNDIKKIL
CCCCCCCHHHHHHHHCHHHHHHHHHCEEEEEEECCCCCCCCCCEEEEECHHCCCCCEEEE
VISKADVVSKEKLFLKATENTYEQIFDEIIITSSTENINIDKLIETIVGFLPETGHYFYD
EEECCCCCCCCCEEEEECHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCEEEC
EESITDQPNRFAIREIIRESVLLKAGQEVPHSVAILVDELEETEDEINIVASIIVERKSQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGIIIGHQGKKISDIKYKSRKQIRELFEKDVNLELFVKVQENRNSASLIKKMGYDKDKY
CCEEEECCCCEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA