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The map label for this gene is trmFO1 [H]

Identifier: 50365058

GI number: 50365058

Start: 269739

End: 271067

Strand: Direct

Name: trmFO1 [H]

Synonym: Mfl242

Alternate gene names: 50365058

Gene position: 269739-271067 (Clockwise)

Preceding gene: 50365057

Following gene: 50365059

Centisome position: 34.01

GC content: 26.79

Gene sequence:

>1329_bases
ATGCAAAAAGAAGTAAATATTATTGGTGCAGGATTAGCTGGTTGCGAAGCTGCTTATTTATTAGCTAACAATGGAGTTAA
AGTAAACTTATTTGAAGTTAAGTCTTTAATGAAAAACGATATTCAAAAAACTAATGATTTAGGAGAATTGGTTTGTTCTA
ATACTTTAAGAAGTAAATCGAAGAAAAATGCTGCAGGGATTTTAAAAAATGAAATGAAGTTATTAAATTCATTAGTTATA
AAAGCTGCATTAGAAAATGAAATACCAGGTGATGATGCATTGAGTGTTGATCGTTTTGGTTTTAGCAAATATATTACTGA
TAAAATTAAGAATCATAAAAATATAAATTTAATTGAACAAGAAGTTTCTGAAGTTGATTATACAAAAGTAACTATCATAG
CCTCAGGTCCGTTGACAACAGATAAATTAGGTAAAAATATTGAATTAATGACAGGTAATGAAAAATTATTCTTTTTAGAT
GCTTCAGCACCTATTATTACAAAGGATAGTATCGATTTTAACAAGGTATATTGAGCAAGTAGACATAATGATGGTAAAGA
TGGTCAATATATATGCATACCACTTAATGAAGAGCAATTTAATGCGTTTGTTGAAGAATTAAAGAATGCTGAAACTATTA
AATTAAAATCATTTGAGAAAGAAATATATTTCAAAGGATGTCAACCAATTGAGCAAATAGCAAAAACAAGTAAAAAAGTA
TTATTAAATGGACCACTATCGCCAAATAATTTAATTGATGAAAATGGAAATACACCTTTTGCTGTTGTTCAATTACGTCA
AGATGATGCAATTGATTCACTTTATAATTTTGTAGGTTTTCAAACTAATATTAAATGACCTGAGCAAAAAAGAATTTTAC
AGACATTACCTGGTCTTGAAAATTTAAATATTGTTAGATTTGGAGTTATGCATAAAAATTATTATATTAATTCTCCTAAA
TTGCTTAATAGATCACTACAAGTCAAAAGGAATAAAAACATTTTCTTTGCAGGTCAAATAACTGGGGTTGAAGGTTATAT
TGAATCTGCAAGTAGTGGTATTCTTACTGCAATCAATGTCTTAGCATATTTAAACAATATAAAAATTGAACAGCCATCAA
GAAAATCTATGTTAGGAGCTCTTAATTTTTATATAACTAATCCAAAGCATGATAAATTAAAACCAATGAAATGTAATTTA
GGAATTTTAGATCAACAAAATAAAAATGCTAAATCAGAGTTCTATTCATTTGATGAATCAGAAAGAGAAATAAGAAGATT
CATAAAGGGTATTAATAATTTTGCAAAGATAGGTGAAAACAATGAATAA

Upstream 100 bases:

>100_bases
GAAAGAACTAAAGTTTGATAATAAACTATTTAAACCGTCTTTAAGATGGTTTTTTTCTTGTTTTAACTTATAATTTTATA
TAGAAGCAAGAGGTTTAATA

Downstream 100 bases:

>100_bases
GAAATGAAATAGTTTATTAACAAATCAAGATCTTTCAAAAAGAATTGAAAACACAATCGAGTTGGCATATAAAACGAATG
AAAAAATTTATCCCTCAAAA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase 1; Folate-dependent tRNA(M-5-U54)-methyltransferase 1 [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MQKEVNIIGAGLAGCEAAYLLANNGVKVNLFEVKSLMKNDIQKTNDLGELVCSNTLRSKSKKNAAGILKNEMKLLNSLVI
KAALENEIPGDDALSVDRFGFSKYITDKIKNHKNINLIEQEVSEVDYTKVTIIASGPLTTDKLGKNIELMTGNEKLFFLD
ASAPIITKDSIDFNKVYWASRHNDGKDGQYICIPLNEEQFNAFVEELKNAETIKLKSFEKEIYFKGCQPIEQIAKTSKKV
LLNGPLSPNNLIDENGNTPFAVVQLRQDDAIDSLYNFVGFQTNIKWPEQKRILQTLPGLENLNIVRFGVMHKNYYINSPK
LLNRSLQVKRNKNIFFAGQITGVEGYIESASSGILTAINVLAYLNNIKIEQPSRKSMLGALNFYITNPKHDKLKPMKCNL
GILDQQNKNAKSEFYSFDESEREIRRFIKGINNFAKIGENNE

Sequences:

>Translated_442_residues
MQKEVNIIGAGLAGCEAAYLLANNGVKVNLFEVKSLMKNDIQKTNDLGELVCSNTLRSKSKKNAAGILKNEMKLLNSLVI
KAALENEIPGDDALSVDRFGFSKYITDKIKNHKNINLIEQEVSEVDYTKVTIIASGPLTTDKLGKNIELMTGNEKLFFLD
ASAPIITKDSIDFNKVY*ASRHNDGKDGQYICIPLNEEQFNAFVEELKNAETIKLKSFEKEIYFKGCQPIEQIAKTSKKV
LLNGPLSPNNLIDENGNTPFAVVQLRQDDAIDSLYNFVGFQTNIK*PEQKRILQTLPGLENLNIVRFGVMHKNYYINSPK
LLNRSLQVKRNKNIFFAGQITGVEGYIESASSGILTAINVLAYLNNIKIEQPSRKSMLGALNFYITNPKHDKLKPMKCNL
GILDQQNKNAKSEFYSFDESEREIRRFIKGINNFAKIGENNE
>Mature_442_residues
MQKEVNIIGAGLAGCEAAYLLANNGVKVNLFEVKSLMKNDIQKTNDLGELVCSNTLRSKSKKNAAGILKNEMKLLNSLVI
KAALENEIPGDDALSVDRFGFSKYITDKIKNHKNINLIEQEVSEVDYTKVTIIASGPLTTDKLGKNIELMTGNEKLFFLD
ASAPIITKDSIDFNKVY*ASRHNDGKDGQYICIPLNEEQFNAFVEELKNAETIKLKSFEKEIYFKGCQPIEQIAKTSKKV
LLNGPLSPNNLIDENGNTPFAVVQLRQDDAIDSLYNFVGFQTNIK*PEQKRILQTLPGLENLNIVRFGVMHKNYYINSPK
LLNRSLQVKRNKNIFFAGQITGVEGYIESASSGILTAINVLAYLNNIKIEQPSRKSMLGALNFYITNPKHDKLKPMKCNL
GILDQQNKNAKSEFYSFDESEREIRRFIKGINNFAKIGENNE

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49353; Mature: 49353

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKEVNIIGAGLAGCEAAYLLANNGVKVNLFEVKSLMKNDIQKTNDLGELVCSNTLRSKS
CCCCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKNAAGILKNEMKLLNSLVIKAALENEIPGDDALSVDRFGFSKYITDKIKNHKNINLIEQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHCCCCCCHHHHH
EVSEVDYTKVTIIASGPLTTDKLGKNIELMTGNEKLFFLDASAPIITKDSIDFNKVYASR
HHHHCCCEEEEEEECCCCCHHHCCCEEEEEECCCEEEEEECCCCEEEECCCCHHHHHHCC
HNDGKDGQYICIPLNEEQFNAFVEELKNAETIKLKSFEKEIYFKGCQPIEQIAKTSKKVL
CCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHCCCEEE
LNGPLSPNNLIDENGNTPFAVVQLRQDDAIDSLYNFVGFQTNIKPEQKRILQTLPGLENL
EECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
NIVRFGVMHKNYYINSPKLLNRSLQVKRNKNIFFAGQITGVEGYIESASSGILTAINVLA
CEEEEEEEECCEEECCHHHHCCCCEEEECCCEEEEEEECCHHHHHHCCCCCHHHHHHHHH
YLNNIKIEQPSRKSMLGALNFYITNPKHDKLKPMKCNLGILDQQNKNAKSEFYSFDESER
HHHCCCCCCCHHHHHHEEEEEEEECCCCCCCCCEECCCCEECCCCCCHHHHHHCCCCHHH
EIRRFIKGINNFAKIGENNE
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQKEVNIIGAGLAGCEAAYLLANNGVKVNLFEVKSLMKNDIQKTNDLGELVCSNTLRSKS
CCCCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKNAAGILKNEMKLLNSLVIKAALENEIPGDDALSVDRFGFSKYITDKIKNHKNINLIEQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHCCCCCCHHHHH
EVSEVDYTKVTIIASGPLTTDKLGKNIELMTGNEKLFFLDASAPIITKDSIDFNKVYASR
HHHHCCCEEEEEEECCCCCHHHCCCEEEEEECCCEEEEEECCCCEEEECCCCHHHHHHCC
HNDGKDGQYICIPLNEEQFNAFVEELKNAETIKLKSFEKEIYFKGCQPIEQIAKTSKKVL
CCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHCCCEEE
LNGPLSPNNLIDENGNTPFAVVQLRQDDAIDSLYNFVGFQTNIKPEQKRILQTLPGLENL
EECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
NIVRFGVMHKNYYINSPKLLNRSLQVKRNKNIFFAGQITGVEGYIESASSGILTAINVLA
CEEEEEEEECCEEECCHHHHCCCCEEEECCCEEEEEEECCHHHHHHCCCCCHHHHHHHHH
YLNNIKIEQPSRKSMLGALNFYITNPKHDKLKPMKCNLGILDQQNKNAKSEFYSFDESER
HHHCCCCCCCHHHHHHEEEEEEEECCCCCCCCCEECCCCEECCCCCCHHHHHHCCCCHHH
EIRRFIKGINNFAKIGENNE
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA