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The map label for this gene is metK [H]

Identifier: 50365056

GI number: 50365056

Start: 266561

End: 267694

Strand: Direct

Name: metK [H]

Synonym: Mfl240

Alternate gene names: 50365056

Gene position: 266561-267694 (Clockwise)

Preceding gene: 50365055

Following gene: 50365057

Centisome position: 33.6

GC content: 30.86

Gene sequence:

>1134_bases
ATGAGAAAATTATTTACTAGCGAAAGTGTTTCAGAAGGACATCCAGACAAAATATGTGATCAGATATCTGATGCGATATT
GGATGAAGTTCTGAAACAAGACCCAAACGCTAAGGTAGCCTGCGAAACATTCGCTACCACAAACTATTTATTAATCGGAG
GGCAGATAACTACAACTGCTTCTGTTGATTATGAAAAAATTGCACGTGATGTATTAAGAAAAATAGGATACAACAACGAT
GCATATGGTATCAATGCTGACACTTGTAAGATTGATATTAGAGTTGAACAACAATCAGCTGATATTGCTTTAGGAATCGA
TTTAGATACTGAAGTTATTGGAGCTGGTGATCAAGGAATTATGTTTGGATATGCAACAAACGAATCTAAAACATTCTTGC
CTTTAGCTATTACTATTTCACATGAATTAGTTTATTTAGCATCAAAGTTAAGAAAAGAAGGAAAATTTAAATGAGCAAGA
CCAGACATGAAATCTCAAGTAACAATTGATTATACAGATGAATCAAATCCTAAAATTGATACAATCTTAATGTCAATTCA
ACATGATGATGAAATGATTGAAGAAGAATTTAAAAAATTCATAAAATCTGAAATAATGGATGTTGTTGCAAAAGAATTTG
AATTGAATACTGATTTTAATGTTTTAATCAATCCAACAGGAAGATTTGTTATTGGTGGACCACAAGGTGATACTGGATTA
ACAGGTAGAAAAATTATTGTTGATACTTATGGTGGATATTCACGTCACGGTGGTGGAGCCTTTTCAGGTAAAGACGCAAC
AAAAGTTGATAGAAGTGCTGCCTATATGGCTAGATATGCTGCTAAAAATTTAGTTGCATCAGGATTAGCTGATAAAATTG
AAATTCAAGTTTCATATGCAATTGGTAAACCAGAACCAGTTTCAATTTTTATTGAAACATTTGGAACAGAAAAAGTTAGC
AAAGAAGTTATAGCAAAAGCATTAAATGAAAATTTTGATTTTTCAGTAAACGAAATTATTAAAAAATTAGATTTAAGAAA
ACCAACATTCTTAAAAACTGCAACTTATGGACACTTTGGAAAAGATGAATTCACATGAGAACAATTGGATAAAGTTAAGA
CAATTAAAAAATAA

Upstream 100 bases:

>100_bases
GAATAAGTATTTAAAAGCAAATTATTTGCTTTTTTTGTTTCAGATTTTTAAACCGATTTAGTATAATTTATATTGATATC
GCAGAAGGGAAAATGGAAAA

Downstream 100 bases:

>100_bases
AAACAAATCACTTTTTAAAAGTGATTTTTTTGTTATAAAATAATAACTAATAAAAGGAGAGATTATATGACAAAAATTAG
ACCACAAGATAATTTCTATG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MRKLFTSESVSEGHPDKICDQISDAILDEVLKQDPNAKVACETFATTNYLLIGGQITTTASVDYEKIARDVLRKIGYNND
AYGINADTCKIDIRVEQQSADIALGIDLDTEVIGAGDQGIMFGYATNESKTFLPLAITISHELVYLASKLRKEGKFKWAR
PDMKSQVTIDYTDESNPKIDTILMSIQHDDEMIEEEFKKFIKSEIMDVVAKEFELNTDFNVLINPTGRFVIGGPQGDTGL
TGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSAAYMARYAAKNLVASGLADKIEIQVSYAIGKPEPVSIFIETFGTEKVS
KEVIAKALNENFDFSVNEIIKKLDLRKPTFLKTATYGHFGKDEFTWEQLDKVKTIKK

Sequences:

>Translated_377_residues
MRKLFTSESVSEGHPDKICDQISDAILDEVLKQDPNAKVACETFATTNYLLIGGQITTTASVDYEKIARDVLRKIGYNND
AYGINADTCKIDIRVEQQSADIALGIDLDTEVIGAGDQGIMFGYATNESKTFLPLAITISHELVYLASKLRKEGKFK*AR
PDMKSQVTIDYTDESNPKIDTILMSIQHDDEMIEEEFKKFIKSEIMDVVAKEFELNTDFNVLINPTGRFVIGGPQGDTGL
TGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSAAYMARYAAKNLVASGLADKIEIQVSYAIGKPEPVSIFIETFGTEKVS
KEVIAKALNENFDFSVNEIIKKLDLRKPTFLKTATYGHFGKDEFT*EQLDKVKTIKK
>Mature_377_residues
MRKLFTSESVSEGHPDKICDQISDAILDEVLKQDPNAKVACETFATTNYLLIGGQITTTASVDYEKIARDVLRKIGYNND
AYGINADTCKIDIRVEQQSADIALGIDLDTEVIGAGDQGIMFGYATNESKTFLPLAITISHELVYLASKLRKEGKFK*AR
PDMKSQVTIDYTDESNPKIDTILMSIQHDDEMIEEEFKKFIKSEIMDVVAKEFELNTDFNVLINPTGRFVIGGPQGDTGL
TGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSAAYMARYAAKNLVASGLADKIEIQVSYAIGKPEPVSIFIETFGTEKVS
KEVIAKALNENFDFSVNEIIKKLDLRKPTFLKTATYGHFGKDEFT*EQLDKVKTIKK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=373, Percent_Identity=50.9383378016086, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI5174529, Length=373, Percent_Identity=51.2064343163539, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=53.7234042553192, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25145633, Length=376, Percent_Identity=49.468085106383, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=48.7935656836461, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17538494, Length=367, Percent_Identity=49.8637602179836, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17538498, Length=370, Percent_Identity=48.9189189189189, Blast_Score=351, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI32565905, Length=370, Percent_Identity=45.1351351351351, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=45.7575757575758, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32565911, Length=324, Percent_Identity=45.0617283950617, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI32565903, Length=298, Percent_Identity=45.9731543624161, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=60.4938271604938, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=84.2105263157895, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=52.3809523809524, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320710, Length=373, Percent_Identity=50.9383378016086, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45552159, Length=372, Percent_Identity=50.8064516129032, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580529, Length=372, Percent_Identity=50.8064516129032, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580537, Length=372, Percent_Identity=50.8064516129032, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580533, Length=372, Percent_Identity=50.8064516129032, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580535, Length=372, Percent_Identity=50.8064516129032, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580531, Length=372, Percent_Identity=50.8064516129032, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580527, Length=372, Percent_Identity=50.5376344086022, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580525, Length=372, Percent_Identity=50.5376344086022, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=48.1283422459893, Blast_Score=320, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=56.4102564102564, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41476; Mature: 41476

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFTSESVSEGHPDKICDQISDAILDEVLKQDPNAKVACETFATTNYLLIGGQITTTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCEEEEEC
SVDYEKIARDVLRKIGYNNDAYGINADTCKIDIRVEQQSADIALGIDLDTEVIGAGDQGI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCEECCCCCCE
MFGYATNESKTFLPLAITISHELVYLASKLRKEGKFKARPDMKSQVTIDYTDESNPKIDT
EEEEECCCCCEEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEE
ILMSIQHDDEMIEEEFKKFIKSEIMDVVAKEFELNTDFNVLINPTGRFVIGGPQGDTGLT
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCC
GRKIIVDTYGGYSRHGGGAFSGKDATKVDRSAAYMARYAAKNLVASGLADKIEIQVSYAI
CCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
GKPEPVSIFIETFGTEKVSKEVIAKALNENFDFSVNEIIKKLDLRKPTFLKTATYGHFGK
CCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCH
DEFTEQLDKVKTIKK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLFTSESVSEGHPDKICDQISDAILDEVLKQDPNAKVACETFATTNYLLIGGQITTTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCEEEEEC
SVDYEKIARDVLRKIGYNNDAYGINADTCKIDIRVEQQSADIALGIDLDTEVIGAGDQGI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCEECCCCCCE
MFGYATNESKTFLPLAITISHELVYLASKLRKEGKFKARPDMKSQVTIDYTDESNPKIDT
EEEEECCCCCEEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEE
ILMSIQHDDEMIEEEFKKFIKSEIMDVVAKEFELNTDFNVLINPTGRFVIGGPQGDTGLT
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCC
GRKIIVDTYGGYSRHGGGAFSGKDATKVDRSAAYMARYAAKNLVASGLADKIEIQVSYAI
CCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
GKPEPVSIFIETFGTEKVSKEVIAKALNENFDFSVNEIIKKLDLRKPTFLKTATYGHFGK
CCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCH
DEFTEQLDKVKTIKK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA