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The map label for this gene is infC

Identifier: 50365004

GI number: 50365004

Start: 217758

End: 218300

Strand: Direct

Name: infC

Synonym: Mfl188

Alternate gene names: 50365004

Gene position: 217758-218300 (Clockwise)

Preceding gene: 50365003

Following gene: 50365005

Centisome position: 27.45

GC content: 29.28

Gene sequence:

>543_bases
ATGGATCAAAGAAGAAACAATTCAAAACCTATTAAAAACCAAGACCCTATTAACGCTTTTATTAGAGCTAGAGAAGTTTT
AATCATTGGAGACAATGGAGAAAAACTTGGACCTTTAAAAAGAAATGAAGCTATTCAATTAGCTGAAGAAAAAGGTTTAG
ATTTAATGCAAGTTGGTCAACAACCTGATGGTTTAGCAATTTGTAAAATTTTAGATTATGGTAAATTCAGATACCAACAA
CAAAAGAAAAACAAAGAAGCTAAGAAAAATCAAGTTAAGGTTGAAAATAAGGAAATTAGATTAACTGTTAATATAGGTCA
ACATGACTTAGTTACAAAAGCTAAAAAAGCTAGAGAGTTTTTAGAAGCTGGGGATAGAGTTAAAATTTCTTTAAAATTCA
AAGGTAGAGAAATCGCATACATGGATTTAGGAAAAGAAACACTGGACAGATTCTACAAAGAAATTGAAGATATAGCTAAA
ATTGAAAAAGAAGCTAAGTTAACTTCAAGATTCTTAGATATGTACGTTGTGCCAAAGAAATAA

Upstream 100 bases:

>100_bases
ACTAAAGTGAAGTGGTAGAATGAAATAATATAAAAATATTATAATGTGTGCTATCTAATTTTTTGACGGCACACATTTTA
TTTATCCAAAGGAGTATTAG

Downstream 100 bases:

>100_bases
TTAGAAAAGTATTGAAAGGAAATTAAGTATTATGCCAAAAATGAAATCAAAAAAATCATTAGCAAAAAGAGTTATTGCTA
AGAAAAACGGTACTTTAAAA

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MDQRRNNSKPIKNQDPINAFIRAREVLIIGDNGEKLGPLKRNEAIQLAEEKGLDLMQVGQQPDGLAICKILDYGKFRYQQ
QKKNKEAKKNQVKVENKEIRLTVNIGQHDLVTKAKKAREFLEAGDRVKISLKFKGREIAYMDLGKETLDRFYKEIEDIAK
IEKEAKLTSRFLDMYVVPKK

Sequences:

>Translated_180_residues
MDQRRNNSKPIKNQDPINAFIRAREVLIIGDNGEKLGPLKRNEAIQLAEEKGLDLMQVGQQPDGLAICKILDYGKFRYQQ
QKKNKEAKKNQVKVENKEIRLTVNIGQHDLVTKAKKAREFLEAGDRVKISLKFKGREIAYMDLGKETLDRFYKEIEDIAK
IEKEAKLTSRFLDMYVVPKK
>Mature_180_residues
MDQRRNNSKPIKNQDPINAFIRAREVLIIGDNGEKLGPLKRNEAIQLAEEKGLDLMQVGQQPDGLAICKILDYGKFRYQQ
QKKNKEAKKNQVKVENKEIRLTVNIGQHDLVTKAKKAREFLEAGDRVKISLKFKGREIAYMDLGKETLDRFYKEIEDIAK
IEKEAKLTSRFLDMYVVPKK

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family

Homologues:

Organism=Escherichia coli, GI1788012, Length=165, Percent_Identity=38.7878787878788, Blast_Score=103, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF3_MESFL (Q6F1S8)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053429.1
- HSSP:   P03000
- ProteinModelPortal:   Q6F1S8
- GeneID:   2898202
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl188
- HOGENOM:   HBG744268
- OMA:   FDRRAPV
- ProtClustDB:   PRK00028
- BioCyc:   MFLO265311:MFL188-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00080
- InterPro:   IPR019813
- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814
- Gene3D:   G3DSA:3.10.20.80
- Gene3D:   G3DSA:3.30.110.10
- PANTHER:   PTHR10938
- TIGRFAMs:   TIGR00168

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N; SSF54364 IF3; SSF55200 IF3

EC number: NA

Molecular weight: Translated: 20847; Mature: 20847

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00938 IF3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQRRNNSKPIKNQDPINAFIRAREVLIIGDNGEKLGPLKRNEAIQLAEEKGLDLMQVGQ
CCCCCCCCCCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHCC
QPDGLAICKILDYGKFRYQQQKKNKEAKKNQVKVENKEIRLTVNIGQHDLVTKAKKAREF
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEECCCHHHHHHHHHHHHH
LEAGDRVKISLKFKGREIAYMDLGKETLDRFYKEIEDIAKIEKEAKLTSRFLDMYVVPKK
HHCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure
MDQRRNNSKPIKNQDPINAFIRAREVLIIGDNGEKLGPLKRNEAIQLAEEKGLDLMQVGQ
CCCCCCCCCCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHCC
QPDGLAICKILDYGKFRYQQQKKNKEAKKNQVKVENKEIRLTVNIGQHDLVTKAKKAREF
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEECCCHHHHHHHHHHHHH
LEAGDRVKISLKFKGREIAYMDLGKETLDRFYKEIEDIAKIEKEAKLTSRFLDMYVVPKK
HHCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA