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The map label for this gene is crr [H]

Identifier: 50365003

GI number: 50365003

Start: 217111

End: 217584

Strand: Direct

Name: crr [H]

Synonym: Mfl187

Alternate gene names: 50365003

Gene position: 217111-217584 (Clockwise)

Preceding gene: 50365001

Following gene: 50365004

Centisome position: 27.37

GC content: 33.33

Gene sequence:

>474_bases
ATGGGATTATTTAGTAAAAAAAATAAACTTGTTGAAATCTATGCACCAGTAGATGGTGAAGTTGTAGGATTAGATAAAGT
TGAAGATGAAGTATTTAGTGGAAAAATGATGGGTGATGGTTTAGCTATCGTTCCAGCAAATGGTGATTTTGTTTCACCAA
TGGCTGGTGAACTAGCAAGTGTTTTCCCAACAAAACATGCATACGGATTTAGAGAAAAATCAGGAGTTGAAGTTTTAGTT
CATATCGGATTAGACACTGTTAACCTTGATGGTGAAGGATTCGAATCATTTGTTAAACAAGGAGACAAAGTAAGCCAAGG
TGACCCAATGGTTAAAGTTGACTTAAAATTTGTTAAACCAAAAGTTCCTTCAATTACAACACCTATTATCGTAACTAACC
CAAATGGTAAAGAAATTACTATTGTTAAAATGGGAAAAGTTAAAAAAGGTGAATTAATCGCAACTGTTGGTTAA

Upstream 100 bases:

>100_bases
TTTATTTTTTATATTTTTTGTTTATATAACTATTATTATGTAAAAATTTAAATGATATAATTTTATTAGCCTAATTAAGT
TTAAAAAGAGGAGAAAAATA

Downstream 100 bases:

>100_bases
TAATATTTACAAATCATTAATTTATTTGTTATAATTTAAATGTTAAAGAAAAAGAAGCACAAGCTCACCACCAACTAAAG
TGAAGTGGTAGAATGAAATA

Product: glucose-specific PTS system IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MGLFSKKNKLVEIYAPVDGEVVGLDKVEDEVFSGKMMGDGLAIVPANGDFVSPMAGELASVFPTKHAYGFREKSGVEVLV
HIGLDTVNLDGEGFESFVKQGDKVSQGDPMVKVDLKFVKPKVPSITTPIIVTNPNGKEITIVKMGKVKKGELIATVG

Sequences:

>Translated_157_residues
MGLFSKKNKLVEIYAPVDGEVVGLDKVEDEVFSGKMMGDGLAIVPANGDFVSPMAGELASVFPTKHAYGFREKSGVEVLV
HIGLDTVNLDGEGFESFVKQGDKVSQGDPMVKVDLKFVKPKVPSITTPIIVTNPNGKEITIVKMGKVKKGELIATVG
>Mature_156_residues
GLFSKKNKLVEIYAPVDGEVVGLDKVEDEVFSGKMMGDGLAIVPANGDFVSPMAGELASVFPTKHAYGFREKSGVEVLVH
IGLDTVNLDGEGFESFVKQGDKVSQGDPMVKVDLKFVKPKVPSITTPIIVTNPNGKEITIVKMGKVKKGELIATVG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=161, Percent_Identity=39.1304347826087, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786894, Length=144, Percent_Identity=36.8055555555556, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790159, Length=123, Percent_Identity=41.4634146341463, Blast_Score=109, Evalue=7e-26,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16735; Mature: 16604

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFSKKNKLVEIYAPVDGEVVGLDKVEDEVFSGKMMGDGLAIVPANGDFVSPMAGELAS
CCCCCCCCCEEEEEECCCCEEEECCCCCHHHHCCEECCCCEEEEECCCCCCCCHHHHHHH
VFPTKHAYGFREKSGVEVLVHIGLDTVNLDGEGFESFVKQGDKVSQGDPMVKVDLKFVKP
HCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEEEECC
KVPSITTPIIVTNPNGKEITIVKMGKVKKGELIATVG
CCCCCCCCEEEECCCCCEEEEEEECCCCCCCEEEECC
>Mature Secondary Structure 
GLFSKKNKLVEIYAPVDGEVVGLDKVEDEVFSGKMMGDGLAIVPANGDFVSPMAGELAS
CCCCCCCCEEEEEECCCCEEEECCCCCHHHHCCEECCCCEEEEECCCCCCCCHHHHHHH
VFPTKHAYGFREKSGVEVLVHIGLDTVNLDGEGFESFVKQGDKVSQGDPMVKVDLKFVKP
HCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEEEECC
KVPSITTPIIVTNPNGKEITIVKMGKVKKGELIATVG
CCCCCCCCEEEECCCCCEEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7703858; 9705652 [H]