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The map label for this gene is map [H]

Identifier: 50364960

GI number: 50364960

Start: 158856

End: 159614

Strand: Direct

Name: map [H]

Synonym: Mfl145

Alternate gene names: 50364960

Gene position: 158856-159614 (Clockwise)

Preceding gene: 50364959

Following gene: 50364961

Centisome position: 20.03

GC content: 32.54

Gene sequence:

>759_bases
ATGGCTGTTACAATAAAATCACCTCAAGAAATTGAAAAAATGCGTGTTGCTGGTCAAGTTTTGGCAGAAGCTATTGACAT
GTTAAAATCAATGATAAAAGTAGGCGTAAATTGCTTAGATTTGGATAAAGAATTTGAAAAATTTATTACTGAAAAAGGAT
GTAAATCTAATTTTAAAAATTATCATGGATACCCAAAAACTATTTGTATATCAATCAATGAGCAATTAGTACATGGAATT
CCACAAGATAGAATTTTAGAAGAAGGAGATTTAGTTTCTGTTGATACAGGGTGTGTTTTCGAAGGATATCATGCTGATAG
TGCATTTAGTATGATAGTAGGCATTGCAAAAGATACAAAACATGATATACTATTAAAAGTCACTGAAGAATGTCTGGATT
TAGCTATTGAAGCTTTAGCTCCAGGGGTTCGTATTGGTACTATTGGGAGCATAATTCAAAACCATGCAGAAAGTTTTGGT
TTTGGAGTTCCAAGAGACTATACAGGACATGGAATTGGTACAGAAATGCATGAAGATCCTTTTATACCAAATTATGGTAA
AAAAGATACAGGAATGCGTTTGCAAGCAGGGATGGTAATTTGTATTGAACCTATGATTCAAATGGGTACTTTCAAAACTA
AACTTGCAGCAGATAATTGAACAGTTCTTTCAGCTGACAAGAGTATGACTGCTCACTTTGAACACACTATCTTAATTACT
GATAGTGGTTATGAAGTATTAACTAAATCAAAAAGATAG

Upstream 100 bases:

>100_bases
AGTAGATTACTATAAAAATAAACCTGGATTTATTCATATTGTAGATAATGCGAACACAACTGCTGAAGAAGTTTATGCAG
AAGTTTTAGGAGCTTTATAG

Downstream 100 bases:

>100_bases
GAGTTGTAGTTTATGGCAAAAGAAGCAGAAATGGAATTTGAAGGTACGGTTGTAGAAGTATTGCCTAATGCAAAATTCAA
AGTACAACTGGAAAACGACA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MAVTIKSPQEIEKMRVAGQVLAEAIDMLKSMIKVGVNCLDLDKEFEKFITEKGCKSNFKNYHGYPKTICISINEQLVHGI
PQDRILEEGDLVSVDTGCVFEGYHADSAFSMIVGIAKDTKHDILLKVTEECLDLAIEALAPGVRIGTIGSIIQNHAESFG
FGVPRDYTGHGIGTEMHEDPFIPNYGKKDTGMRLQAGMVICIEPMIQMGTFKTKLAADNWTVLSADKSMTAHFEHTILIT
DSGYEVLTKSKR

Sequences:

>Translated_252_residues
MAVTIKSPQEIEKMRVAGQVLAEAIDMLKSMIKVGVNCLDLDKEFEKFITEKGCKSNFKNYHGYPKTICISINEQLVHGI
PQDRILEEGDLVSVDTGCVFEGYHADSAFSMIVGIAKDTKHDILLKVTEECLDLAIEALAPGVRIGTIGSIIQNHAESFG
FGVPRDYTGHGIGTEMHEDPFIPNYGKKDTGMRLQAGMVICIEPMIQMGTFKTKLAADN*TVLSADKSMTAHFEHTILIT
DSGYEVLTKSKR
>Mature_251_residues
AVTIKSPQEIEKMRVAGQVLAEAIDMLKSMIKVGVNCLDLDKEFEKFITEKGCKSNFKNYHGYPKTICISINEQLVHGIP
QDRILEEGDLVSVDTGCVFEGYHADSAFSMIVGIAKDTKHDILLKVTEECLDLAIEALAPGVRIGTIGSIIQNHAESFGF
GVPRDYTGHGIGTEMHEDPFIPNYGKKDTGMRLQAGMVICIEPMIQMGTFKTKLAADN*TVLSADKSMTAHFEHTILITD
SGYEVLTKSKR

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=247, Percent_Identity=38.8663967611336, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI40385867, Length=248, Percent_Identity=31.8548387096774, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI11559925, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1786364, Length=253, Percent_Identity=41.501976284585, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1788728, Length=213, Percent_Identity=29.5774647887324, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996291, Length=247, Percent_Identity=36.8421052631579, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323273, Length=252, Percent_Identity=34.9206349206349, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21355531, Length=243, Percent_Identity=37.4485596707819, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24583427, Length=247, Percent_Identity=34.412955465587, Blast_Score=149, Evalue=1e-36,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27597; Mature: 27466

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTIKSPQEIEKMRVAGQVLAEAIDMLKSMIKVGVNCLDLDKEFEKFITEKGCKSNFKN
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHH
YHGYPKTICISINEQLVHGIPQDRILEEGDLVSVDTGCVFEGYHADSAFSMIVGIAKDTK
CCCCCCEEEEEECHHHHHCCCHHHHCCCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCC
HDILLKVTEECLDLAIEALAPGVRIGTIGSIIQNHAESFGFGVPRDYTGHGIGTEMHEDP
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
FIPNYGKKDTGMRLQAGMVICIEPMIQMGTFKTKLAADNTVLSADKSMTAHFEHTILITD
CCCCCCCCCCCCEEECCEEEEEHHHHHHCCCHHHHCCCCEEEECCCCCEEEEEEEEEEEC
SGYEVLTKSKR
CCHHHHCCCCC
>Mature Secondary Structure 
AVTIKSPQEIEKMRVAGQVLAEAIDMLKSMIKVGVNCLDLDKEFEKFITEKGCKSNFKN
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHH
YHGYPKTICISINEQLVHGIPQDRILEEGDLVSVDTGCVFEGYHADSAFSMIVGIAKDTK
CCCCCCEEEEEECHHHHHCCCHHHHCCCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCC
HDILLKVTEECLDLAIEALAPGVRIGTIGSIIQNHAESFGFGVPRDYTGHGIGTEMHEDP
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
FIPNYGKKDTGMRLQAGMVICIEPMIQMGTFKTKLAADNTVLSADKSMTAHFEHTILITD
CCCCCCCCCCCCEEECCEEEEEHHHHHHCCCHHHHCCCCEEEECCCCCEEEEEEEEEEEC
SGYEVLTKSKR
CCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476192 [H]