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The map label for this gene is mdeA [H]

Identifier: 49188243

GI number: 49188243

Start: 5140199

End: 5141497

Strand: Reverse

Name: mdeA [H]

Synonym: BAS5258

Alternate gene names: 49188243

Gene position: 5141497-5140199 (Counterclockwise)

Preceding gene: 49188245

Following gene: 49188242

Centisome position: 98.33

GC content: 39.49

Gene sequence:

>1299_bases
ATGGGAGAATCATGGGGGAAAGGAACGATTTGTGTGCAAGGTGGCTATACGCCAAAGAATGGAGAACCGCGTGTTTTACC
GCTTTATCAAAGCACGACGTATAAATATGATACTTCGGATGATTTAGCAGCATTATTTAATTTAGAGGCAGAAGGATATA
TTTATACGCGTATTGGTAATCCTACTTTAGCTGCATTTGAGCAAAAGCTGGCGGAATTAGAGGGCGGTGTAGGGGCTGTT
GCAACAGCTTCTGGGCAGGCTGCTATTATGCTCGCCGTTTTAAATATTTGTAGTAGCGGAGACCACTTGCTTTGTTCTTC
CACAGTTTATGGCGGGACGTTTAATTTATTTGGGGTGAGTTTGCGTAAGCTTGGTATTGATGTTACGTTCTTTAATCCGA
ATTTAACTGCTGATGAAATTGTGGCCCTTGCTAATGATAAAACGAAACTCGTTTATGCAGAATCGCTAGGAAATCCAGCG
ATGAACGTTTTAAATTTCAAAGAGTTTTCAGATGCAGCGAAAGAGCTAGAAGTACCTTTTATCGTGGATAATACATTAGC
AACTCCTTATTTATGCCAAGCATTTGAACACGGAGCAAATATTATCGTTCATTCTACGACGAAATATATTGATGGTCATG
CAAGTTCTTTGGGCGGCATTGTCATTGATGGAGGAAACTTCGATTGGACAAATGGAAAATATCCTGAACTTGTTGAACCG
GATCCAAGTTATCATGGCGTAAGTTACGTTCAAAATTTTGGAGCGGCAGCTTATATTGTGAAGGCACGTGTTCAATTATT
AAGAGACTATGGAAACTGTATGAGCCCATTTAATGCGTATATTAGCAATATTGGCTTAGAAACATTGCATTTACGAATGG
AGCGTCATAGTGAAAATGCTCTCGCAGTTGCTAAATGGCTTGCTGATCATGAACGTATTGAATGGGTGAATTATCCGGGA
TTAGATAGTAATGAAAATTATTCGTTAGCACAAAAGTATTTGAAAAAAGGTGCGAGTGGTGTTTTAACATTCGGCATTAA
GGGAGGATTAGAAGCAGCAAAAGAATTTATCGCAAATGTAAAACTAGCAACTCTTGTAACGCATGTAGCTGATGCACGAA
CTTGCGTTATACATCCTGCTAGTACAACGCATAGACAATTAAGTGCTGAAGATCAACGTTTAGCTGGCGTCACATCAGAT
TTAATTCGTTTATCAGTTGGCATAGAAGATGTTTCTGATATTATTGCAGATTTAGAAGCAGCGCTAGTCGGAGGCAAACA
ACATGCCGATCATAATTGA

Upstream 100 bases:

>100_bases
GATAAGAAAGAGCCTTTAGAGCGTGTTTTCAAACTGCTCCTTTCTTGTTTTCAGGAAAGGGGCAGTTTTTTATTTTGTAT
AAAAGAAAGGAGAATGAGAA

Downstream 100 bases:

>100_bases
TAAAGATTTACCAGCTCGCAAAGTATTGCAGGAGGAAAATATTTTTGTAATGACAAAAGAGCGAGCAGAAACACAAGATA
TCCGGGCATTGAAAATTGCT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTLAAFEQKLAELEGGVGAV
ATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPA
MNVLNFKEFSDAAKELEVPFIVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALAVAKWLADHERIEWVNYPG
LDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANVKLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSD
LIRLSVGIEDVSDIIADLEAALVGGKQHADHN

Sequences:

>Translated_432_residues
MGESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTLAAFEQKLAELEGGVGAV
ATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPA
MNVLNFKEFSDAAKELEVPFIVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALAVAKWLADHERIEWVNYPG
LDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANVKLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSD
LIRLSVGIEDVSDIIADLEAALVGGKQHADHN
>Mature_431_residues
GESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTLAAFEQKLAELEGGVGAVA
TASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPAM
NVLNFKEFSDAAKELEVPFIVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEPD
PSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALAVAKWLADHERIEWVNYPGL
DSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANVKLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSDL
IRLSVGIEDVSDIIADLEAALVGGKQHADHN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=30.5210918114144, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI25453487, Length=401, Percent_Identity=28.1795511221945, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=27.2952853598015, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=31.1392405063291, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789383, Length=374, Percent_Identity=28.6096256684492, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=31.3725490196078, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=408, Percent_Identity=34.8039215686275, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=408, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI193202265, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=42.7184466019417, Blast_Score=318, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6319307, Length=402, Percent_Identity=32.089552238806, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=28.6585365853659, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321254, Length=438, Percent_Identity=24.8858447488584, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=406, Percent_Identity=23.8916256157635, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=33.4123222748815, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46619; Mature: 46488

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHEECCCCCEEEEEECC
PTLAAFEQKLAELEGGVGAVATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVS
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECEECCCCHHHHHHH
LRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPAMNVLNFKEFSDAAKELEVPF
HHHHCEEEEEECCCCCHHHEEEEECCCEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCE
IVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
EECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENA
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEHHHCCCCH
LAVAKWLADHERIEWVNYPGLDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANV
HHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
KLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSDLIRLSVGIEDVSDIIADLEA
HHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
ALVGGKQHADHN
HHCCCCCCCCCC
>Mature Secondary Structure 
GESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGN
CCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHEECCCCCEEEEEECC
PTLAAFEQKLAELEGGVGAVATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVS
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECEECCCCHHHHHHH
LRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPAMNVLNFKEFSDAAKELEVPF
HHHHCEEEEEECCCCCHHHEEEEECCCEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCE
IVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
EECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENA
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEHHHCCCCH
LAVAKWLADHERIEWVNYPGLDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANV
HHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
KLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSDLIRLSVGIEDVSDIIADLEA
HHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
ALVGGKQHADHN
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]