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The map label for this gene is tyrS2 [H]

Identifier: 49187924

GI number: 49187924

Start: 4818783

End: 4820042

Strand: Reverse

Name: tyrS2 [H]

Synonym: BAS4936

Alternate gene names: 49187924

Gene position: 4820042-4818783 (Counterclockwise)

Preceding gene: 49187925

Following gene: 49187920

Centisome position: 92.18

GC content: 38.02

Gene sequence:

>1260_bases
ATGAATATCATTGATGAATTAGAATGGCGCGGTGCTGTTAATCAACAGACTGACGAAGAAGGTTTACGTAAGCTAGTAGA
GGAGAAAAAGATTTCGCTATACTGCGGAGTTGATCCGACTGGTGATAGTATGCATATCGGACATTTGATTCCGTTTATGA
TGATGAAAAGATTCCAATTAGCTGGTCATCATCCAGTTATTTTAATCGGCGGTGCAACAGGAACAATTGGTGATCCGAGT
GGGCGTCAATCAGAACGCCAACTGCAAACGTTAGAAGTAGTGCAGCATAATGTGGATGCATTAACAGCGCAAATGAAAAA
GCTATTTGATTTTGGCGGAAATAGCGAAGTGAAGATGGTAAATAACTACGACTGGACACATGAAATAAATATTATTGAGT
TTTTACGTGATTACGGGAAAAACTTTAGCATTAATAGCATGTTAGCAAAAGACATCGTAGCAAGTCGTTTAGATACAGGA
ATTTCTTTCACAGAATTTACGTACCAAATCTTGCAAGCGATGGACTTCCATCATTTATACACGAAAGAAGATGTTCAACT
ACAAATTGGTGGTAGTGACCAATGGGGAAATATTACGAGTGGTTTAGATTTAATTCGTAAGTTAGAAGGGCACGAAGCAA
AAGTATTTGGATTGACGATTCCGTTACTATTGAAATCAGATGGTACAAAGTTTGGTAAATCAGCAGGTGGTGCGGTTTGG
CTTGATCCTGAAAAAACAACACCATTTGAATTTTACCAGTTCTGGGTAAATACAGATGACCGTGATGTCGTTAAATACTT
AAAATACTTTACGTTCTTAACGAAAGAGCGTATTGATGAATTGGCAGTGAAGGTAGAAACAGAGCCTCATAAACGTGAAG
CGCAAAAAGTATTAGCGGAAGAAATGACGAAATTCGTTCATGGAGAAGAAGCATTGCTGCAAGCTGTGAAAATTACAGCA
GCGTTATTTAGCGGAGATATTAAATCGTTAACAGCTGATGAAATTGAGCAAGGCTTTAAAGAAATGCCAACATTCCAGTC
TTCAAAAGAGACGAAAAACATCGTAGAATGGCTAGTTGATTTAGGAATTGAACCATCTAGACGACAAGCGCGTGAAGATA
TTAATAACGGAGCTATTTCTATGAATGGCGAAAAAGTAACGGATGTAGGTACGGATGTTACGGTAGAAAATTCCTTTGAT
GGACGATTTATTATTATTCGTAAAGGGAAGAAGAACTACAGCCTTGTGAAGTTAGGATAA

Upstream 100 bases:

>100_bases
GCTATTTATACAAACTGGGTGGTAACACGGTGAATAATCGTCCCTATGTCATAGACATAGGGATTTTTTGTTTTTAAAAA
AATAAAGGGGATGACAAAAG

Downstream 100 bases:

>100_bases
TGAAGAAGGTGGAAAAATTATATCGGCGATTTTTTGAATATATAGACCGTAACTCGAATTATATCGGCGATTTTTAAAAT
ATATCGATCGTAATTTGAAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 2; TyrRS 2 [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNIIDELEWRGAVNQQTDEEGLRKLVEEKKISLYCGVDPTGDSMHIGHLIPFMMMKRFQLAGHHPVILIGGATGTIGDPS
GRQSERQLQTLEVVQHNVDALTAQMKKLFDFGGNSEVKMVNNYDWTHEINIIEFLRDYGKNFSINSMLAKDIVASRLDTG
ISFTEFTYQILQAMDFHHLYTKEDVQLQIGGSDQWGNITSGLDLIRKLEGHEAKVFGLTIPLLLKSDGTKFGKSAGGAVW
LDPEKTTPFEFYQFWVNTDDRDVVKYLKYFTFLTKERIDELAVKVETEPHKREAQKVLAEEMTKFVHGEEALLQAVKITA
ALFSGDIKSLTADEIEQGFKEMPTFQSSKETKNIVEWLVDLGIEPSRRQAREDINNGAISMNGEKVTDVGTDVTVENSFD
GRFIIIRKGKKNYSLVKLG

Sequences:

>Translated_419_residues
MNIIDELEWRGAVNQQTDEEGLRKLVEEKKISLYCGVDPTGDSMHIGHLIPFMMMKRFQLAGHHPVILIGGATGTIGDPS
GRQSERQLQTLEVVQHNVDALTAQMKKLFDFGGNSEVKMVNNYDWTHEINIIEFLRDYGKNFSINSMLAKDIVASRLDTG
ISFTEFTYQILQAMDFHHLYTKEDVQLQIGGSDQWGNITSGLDLIRKLEGHEAKVFGLTIPLLLKSDGTKFGKSAGGAVW
LDPEKTTPFEFYQFWVNTDDRDVVKYLKYFTFLTKERIDELAVKVETEPHKREAQKVLAEEMTKFVHGEEALLQAVKITA
ALFSGDIKSLTADEIEQGFKEMPTFQSSKETKNIVEWLVDLGIEPSRRQAREDINNGAISMNGEKVTDVGTDVTVENSFD
GRFIIIRKGKKNYSLVKLG
>Mature_419_residues
MNIIDELEWRGAVNQQTDEEGLRKLVEEKKISLYCGVDPTGDSMHIGHLIPFMMMKRFQLAGHHPVILIGGATGTIGDPS
GRQSERQLQTLEVVQHNVDALTAQMKKLFDFGGNSEVKMVNNYDWTHEINIIEFLRDYGKNFSINSMLAKDIVASRLDTG
ISFTEFTYQILQAMDFHHLYTKEDVQLQIGGSDQWGNITSGLDLIRKLEGHEAKVFGLTIPLLLKSDGTKFGKSAGGAVW
LDPEKTTPFEFYQFWVNTDDRDVVKYLKYFTFLTKERIDELAVKVETEPHKREAQKVLAEEMTKFVHGEEALLQAVKITA
ALFSGDIKSLTADEIEQGFKEMPTFQSSKETKNIVEWLVDLGIEPSRRQAREDINNGAISMNGEKVTDVGTDVTVENSFD
GRFIIIRKGKKNYSLVKLG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=37.2262773722628, Blast_Score=258, Evalue=8e-69,
Organism=Escherichia coli, GI1787925, Length=420, Percent_Identity=50.4761904761905, Blast_Score=426, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145802, Length=394, Percent_Identity=36.8020304568528, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI25145805, Length=331, Percent_Identity=35.0453172205438, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=37.5854214123007, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI20130383, Length=415, Percent_Identity=38.5542168674699, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47351; Mature: 47351

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDELEWRGAVNQQTDEEGLRKLVEEKKISLYCGVDPTGDSMHIGHLIPFMMMKRFQL
CCCHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHHPVILIGGATGTIGDPSGRQSERQLQTLEVVQHNVDALTAQMKKLFDFGGNSEVKMV
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NNYDWTHEINIIEFLRDYGKNFSINSMLAKDIVASRLDTGISFTEFTYQILQAMDFHHLY
CCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
TKEDVQLQIGGSDQWGNITSGLDLIRKLEGHEAKVFGLTIPLLLKSDGTKFGKSAGGAVW
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEE
LDPEKTTPFEFYQFWVNTDDRDVVKYLKYFTFLTKERIDELAVKVETEPHKREAQKVLAE
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
EMTKFVHGEEALLQAVKITAALFSGDIKSLTADEIEQGFKEMPTFQSSKETKNIVEWLVD
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LGIEPSRRQAREDINNGAISMNGEKVTDVGTDVTVENSFDGRFIIIRKGKKNYSLVKLG
CCCCCCHHHHHHHHCCCEEEECCCEEEECCCCEEEECCCCCEEEEEEECCCCCCEEEEC
>Mature Secondary Structure
MNIIDELEWRGAVNQQTDEEGLRKLVEEKKISLYCGVDPTGDSMHIGHLIPFMMMKRFQL
CCCHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHHPVILIGGATGTIGDPSGRQSERQLQTLEVVQHNVDALTAQMKKLFDFGGNSEVKMV
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NNYDWTHEINIIEFLRDYGKNFSINSMLAKDIVASRLDTGISFTEFTYQILQAMDFHHLY
CCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
TKEDVQLQIGGSDQWGNITSGLDLIRKLEGHEAKVFGLTIPLLLKSDGTKFGKSAGGAVW
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEE
LDPEKTTPFEFYQFWVNTDDRDVVKYLKYFTFLTKERIDELAVKVETEPHKREAQKVLAE
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
EMTKFVHGEEALLQAVKITAALFSGDIKSLTADEIEQGFKEMPTFQSSKETKNIVEWLVD
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LGIEPSRRQAREDINNGAISMNGEKVTDVGTDVTVENSFDGRFIIIRKGKKNYSLVKLG
CCCCCCHHHHHHHHCCCEEEECCCEEEECCCCEEEECCCCCEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]