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The map label for this gene is vanS [H]

Identifier: 49187735

GI number: 49187735

Start: 4626300

End: 4627406

Strand: Reverse

Name: vanS [H]

Synonym: BAS4744

Alternate gene names: 49187735

Gene position: 4627406-4626300 (Counterclockwise)

Preceding gene: 49187736

Following gene: 49187734

Centisome position: 88.5

GC content: 34.06

Gene sequence:

>1107_bases
ATGGATAACATGATGAAAAGCTTTCGATTTAAAATGATTGCCTTATTTGCGTTAAGTATGGTGCTCGCTGCAACTGTAAC
TTATATAATTTATAAAGGATTGCAGTTGTATTATAAAACGATGGTTCGCTATGAAGAGCCTTTGGCACAATTTAGGTCAA
TCGTAAGGGAATTTGGAGACATTAACTTCTTTTTAATTTTCTTTATCCCGTTATCTATTATCTTTTTCTTCTTTTTGACA
AAACCGTATTTAAAGTATTTTAGTGAGATTTCTAACGGGATTCATCATCTTGCGAACGGTAACTTTACGAATAGAGTTCA
AGTTTCATCAAATGATGAGTTTGGAGACATCGCGCGTGAAATAAATGTTGCGAGTGAAAAGTTAAAAGAAGCTGTGGAAA
GAGGGGACTTTGCTGAAAGTAGTAAAGACCAGCTTGTTGTGAATTTAGCCCATGATTTAAGGACACCATTAACATCCGTG
TTAGGGTATTTAGATTTAATTCTTAAAGATGAGAATTTAACAAAAGAACAAATTAAACATTTTTCCACGATTGCATATAC
GAAATCGCAAAGGCTTGAAAGCTTAATTGATGAATTATTTGAAATTACACGTATGAATTATGGTATGTTACAACTAAATA
AAAAGCCGATTGATATAAGTGAGCTGCTTATACAGCTAGATGAGGAATTGTATCCGTTATTAGAAAAACATCATTTAGAA
GCTAGATTGAATGTTGATCCGCATTTGCCGATTAACGGTGATGGAAAATTGTTAGCTAGAGTATTTGAAAACTTGTTAAC
AAATGCCGTTCGCTACGGATATGATGGGCAATTTGTTGATCTGAATGGGTATATCGATAATGAAGAAGTTGTCGTACAAG
TTATGAATTACGGAGATAGCATTCCAGAAGAAGATTTACCGTATCTTTTTGATATGTTTTATACAGGTGATAAATCGAGA
ACTGAGAACCGTGGCGGGACAGGCCTTGGACTATTTATTGCGAAAAATATTGTCGAGCAACATAACGGTACGATTTCTGC
TGAAAGTAATGTAGTTAGAACGATATTTGAAGTACGATTGCCAAAAGACAAGAAGTTGACAATTTAA

Upstream 100 bases:

>100_bases
ACAGTTATGGTACATATTCGTACGTTGCGGAAAAAGCTTGGGGAAGATAAAAGAAAAGATAAGCTAATAAAAACAGTGTG
GGGAGTAGGGTATACTTTCA

Downstream 100 bases:

>100_bases
TAAAAATTTAAACTTTCCCCCACTTTTTATTTTAATAGTTTTTTCTATTCTAGACGTAAGTTACGTTAAGGAGGAACTAG
AAATGAAAAAGTGGGTATTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MDNMMKSFRFKMIALFALSMVLAATVTYIIYKGLQLYYKTMVRYEEPLAQFRSIVREFGDINFFLIFFIPLSIIFFFFLT
KPYLKYFSEISNGIHHLANGNFTNRVQVSSNDEFGDIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLQLNKKPIDISELLIQLDEELYPLLEKHHLE
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNEEVVVQVMNYGDSIPEEDLPYLFDMFYTGDKSR
TENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTIFEVRLPKDKKLTI

Sequences:

>Translated_368_residues
MDNMMKSFRFKMIALFALSMVLAATVTYIIYKGLQLYYKTMVRYEEPLAQFRSIVREFGDINFFLIFFIPLSIIFFFFLT
KPYLKYFSEISNGIHHLANGNFTNRVQVSSNDEFGDIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLQLNKKPIDISELLIQLDEELYPLLEKHHLE
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNEEVVVQVMNYGDSIPEEDLPYLFDMFYTGDKSR
TENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTIFEVRLPKDKKLTI
>Mature_368_residues
MDNMMKSFRFKMIALFALSMVLAATVTYIIYKGLQLYYKTMVRYEEPLAQFRSIVREFGDINFFLIFFIPLSIIFFFFLT
KPYLKYFSEISNGIHHLANGNFTNRVQVSSNDEFGDIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLQLNKKPIDISELLIQLDEELYPLLEKHHLE
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNEEVVVQVMNYGDSIPEEDLPYLFDMFYTGDKSR
TENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTIFEVRLPKDKKLTI

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=289, Percent_Identity=29.757785467128, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789149, Length=333, Percent_Identity=26.7267267267267, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=28.4518828451883, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082128, Length=360, Percent_Identity=27.7777777777778, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788713, Length=295, Percent_Identity=26.4406779661017, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=28.82096069869, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=28.3261802575107, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786600, Length=215, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=29.3103448275862, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790346, Length=267, Percent_Identity=25.0936329588015, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787894, Length=291, Percent_Identity=24.0549828178694, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=249, Percent_Identity=28.5140562248996, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788279, Length=330, Percent_Identity=26.969696969697, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=229, Percent_Identity=23.5807860262009, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=25.5707762557078, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=27.3858921161826, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789808, Length=205, Percent_Identity=26.8292682926829, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42412; Mature: 42412

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNMMKSFRFKMIALFALSMVLAATVTYIIYKGLQLYYKTMVRYEEPLAQFRSIVREFGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
INFFLIFFIPLSIIFFFFLTKPYLKYFSEISNGIHHLANGNFTNRVQVSSNDEFGDIARE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHH
INVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKEQIKH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FSTIAYTKSQRLESLIDELFEITRMNYGMLQLNKKPIDISELLIQLDEELYPLLEKHHLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNEEVVVQVMNYGDS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCC
IPEEDLPYLFDMFYTGDKSRTENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTIFEVRL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
PKDKKLTI
CCCCCCCC
>Mature Secondary Structure
MDNMMKSFRFKMIALFALSMVLAATVTYIIYKGLQLYYKTMVRYEEPLAQFRSIVREFGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
INFFLIFFIPLSIIFFFFLTKPYLKYFSEISNGIHHLANGNFTNRVQVSSNDEFGDIARE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHH
INVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKEQIKH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FSTIAYTKSQRLESLIDELFEITRMNYGMLQLNKKPIDISELLIQLDEELYPLLEKHHLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNEEVVVQVMNYGDS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCC
IPEEDLPYLFDMFYTGDKSRTENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTIFEVRL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
PKDKKLTI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]