Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 49187547

GI number: 49187547

Start: 4458681

End: 4459856

Strand: Reverse

Name: mdeA [H]

Synonym: BAS4553

Alternate gene names: 49187547

Gene position: 4459856-4458681 (Counterclockwise)

Preceding gene: 49187550

Following gene: 49187545

Centisome position: 85.3

GC content: 39.8

Gene sequence:

>1176_bases
ATGAAAAAGAAGCATATGGAGACAGCGTTAATTCATCACGGTTATACATCAGAGGAACATAAAGGAAGTTTAACACCGCC
TTTATTTCAAACGTCTACATTTACATTTGAGACTGCGCAGCAAGGAGAGGCGAGTTTTGCGGGAGTGGATCCATCTTATA
TTTACTCAAGACTTGGAAATCCGACTGTGAAATTATTTGAAGAACGTATGGCGGTGTTAGAAGGAGGAGAAGAAGCGCTT
GCTTTCGGGTCCGGTATGGCAGCTATTTCCGCAACGTTAATTGGTTTTCTAAAAGCTGGAGATCATATTATTTGTTCAAA
TGGATTATACGGATGCACGTACGGCTTTTTGGAAGTGTTGGAAGAAAAATTTATGATTACGCATTCGTTTTGTGATATGG
AGACAGAGGCTGATATTGAAAATAAAATTCGCCCAAATACAAAGCTTATTTTCGTTGAAACACCGATTAATCCGACAATG
AAATTAATTGATTTAAAACAAGTGATTCGGGTTGCGAAGCGAAATGGTTTACTTGTCATTGTTGACAATACGTTTTGTTC
ACCTTATTTACAAAGACCGCTTGAACTTGGCTGTGACGCCGTTGTTCATAGTGCGACAAAATATATTGGTGGCCACGGTG
ATGTTGTGGCGGGTGTAACAATTTGTAAAACGAGAGCGTTAGCTGAAAAAATCCGCCCGATGCGAAAAGATATCGGCGGT
ATTATGGCACCGTTTGATGCATGGTTATTGTTACGCGGATTAAAGACACTAGCGGTAAGAATGGACCGCCATTGTGATAA
TGCAGAAAAAATTGTATCGTTCCTAAAAAATCATGATGCGGTAGAAGGTGTTTGGTATCCAGAAGGGGAGTTAGCATCTC
GCCAAATGAAACGGGGCGGCGTTATATCTTTTTCAATTAAAGGCGGGAAAGAAGAGACGCAAGCGTTTATTAATGACCTC
TACTTTATTACAATTGCAGTCAGTTTGGGAGATACAGAAACGTTAATTCAGCACCCGGCAACGATGACGCACGCTGCGAT
TCCAGCTGAGTTAAGACAAGAAATGGGTATTTATGATAATTTAATACGTTTATCTGTCGGTTTAGAATCGTGGGAGGATA
TCGTTTCTGATTTAGAGCAAGCATTAAAGAAAATATCTACTGTTAGTAATCAATAA

Upstream 100 bases:

>100_bases
TAGAAACACAAATAAAACGCTTACATAAAATGAAGTAAAACGTTCATCAAATTTTTAATGATGAATATGCGGCAATAAAA
ACATAAAAGGGGATGTTTTT

Downstream 100 bases:

>100_bases
AAAAAGAGCTAGCTATCGGTTTTGTACGTAATGTACTCAATCGATAACTAGCTCTTTTTTGTTACATATGTTTTAGGAGT
GTTTCCACAAATTTTTCTAA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGVISFSIKGGKEETQAFINDL
YFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Sequences:

>Translated_391_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGVISFSIKGGKEETQAFINDL
YFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
>Mature_391_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGVISFSIKGGKEETQAFINDL
YFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=40.6169665809769, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI299473759, Length=389, Percent_Identity=36.2467866323907, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=35.5670103092784, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=36.4102564102564, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=27.9596977329975, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=40.2564102564103, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=41.2820512820513, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=39.4594594594595, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI193202265, Length=88, Percent_Identity=37.5, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=38.8746803069054, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6323333, Length=446, Percent_Identity=30.0448430493274, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=30.5084745762712, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=30.25, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=294, Percent_Identity=30.952380952381, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922554, Length=385, Percent_Identity=38.1818181818182, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43221; Mature: 43221

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEERMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHCCC
VISFSIKGGKEETQAFINDLYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDN
EEEEEECCCHHHHHHHHHHEEEEEEEEECCCHHHHHHCCCHHHHHHCCHHHHHHCCHHHH
LIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEERMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEADIENKIRPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHCCC
VISFSIKGGKEETQAFINDLYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDN
EEEEEECCCHHHHHHHHHHEEEEEEEEECCCHHHHHHCCCHHHHHHCCHHHHHHCCHHHH
LIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]