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The map label for this gene is tyrS1

Identifier: 49187550

GI number: 49187550

Start: 4461570

End: 4462826

Strand: Reverse

Name: tyrS1

Synonym: BAS4556

Alternate gene names: 49187550

Gene position: 4462826-4461570 (Counterclockwise)

Preceding gene: 49187551

Following gene: 49187547

Centisome position: 85.35

GC content: 39.22

Gene sequence:

>1257_bases
ATGGGTATTTTACAAGATCTTGAATTTCGCGGTCTAATTAATCAGCAAACAGACGCTGAGGGCCTTGAGCAATTATTAGA
AAAAGAAAGCGTTAAATTATACTGTGGTTTCGACCCGACAGCGGATAGCTTACACATCGGTCATATGTTACCAGTATTAA
TGTTACGTCGTTTCCAATTAGCTGGTCACCAACCAATCGCACTTGTTGGCGGTGGTACTGGTATGATCGGTGATCCAAGT
GGTAAAAAAGCAGAGCGTACATTAAATACGAAAGATACAGTTGCTTACTACACAGAAAGCATTAAAAACCAACTTTCAAA
CTTCTTAGAGTTCGAAAACGTGGAAAACCCAGCAACGATGGCTAACAACTATGACTGGCTTGGTAACTTAGATGTCATTT
CATTCTTACGCGATATCGGTAAAAACTTCGGTTTAAACTATATGCTAGCAAAAGATACAGTAGCATCTCGTTTAGAAACT
GGTATTTCATTTACTGAGTTTAGTTACATGATTTTACAATCATACGACTTCTTAAACTTATACCAACACCATAATTGCCG
CCTACAAATCGGTGGTAGTGACCAATGGGGTAACATTACAGCTGGTCTTGAATTAATCCGTAAATCAGAAGAAGATGCGA
AAGCATTCGGTTTAACAATTCCACTTGTTACAAAATCTGACGGTACGAAGTTTGGTAAAACAGAAGGCGGCGCAATTTGG
TTAGACCCAGAGAAAACAACTCCTTACGAGTTCTACCAATTCTGGATTAACACAGATGACCGTGATGTTGTTAAATACTT
AAAATACTTCACATTCTTATCTCATGAAGAAATTCTTGAGCTTGAAAAGCAAGTAGCTGAAGCGCCAGAAAAACGTGCAG
CACAAAAAGCATTAGGTGCAGAAATGACAAAACTTGTTCACGGCGAAGAAGCATTAGAGCAAGCGATTAAAATTTCAGCT
GCATTATTCAGCGGTTCTGTAGCAGAACTGACTGCAAGCGAAATCGAGCAAGGATTCAAAGACGTACCATCTGTAGAACG
TACTGCAGAAGATACAGTATTAATCGACTTACTTGTAGAAAGCAAAATCTCTCCATCAAAACGTCAAGCACGTGAAGATG
TAACGAACGGCGCAATCTACGTAAACGGTGAGCGTACACAAGCATTAGACTACGTTGTAACAGAAAAAGACCGCATCGAA
GGTAAATTCACAATCATTCGCCGCGGTAAGAAGAAATATTTCTTAATTCGTTACTAA

Upstream 100 bases:

>100_bases
GGTAACGCGATAATCAAAATCGTCCCTTATTTGAAGGGGCGATTTTTTTATGTTTTTAACCTTCACGCCAGTAATGAACT
TAAAGGAGAGTATGTAGGAT

Downstream 100 bases:

>100_bases
TTAGAAACGAATATAAACAAATAGTACCCCGAATTGTAGGCTGAAAAGTTTATGATTCGGGGTTTTTATGTATGGATTTA
AATCGCCGGATCTTTTCTTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPVLMLRRFQLAGHQPIALVGGGTGMIGDPS
GKKAERTLNTKDTVAYYTESIKNQLSNFLEFENVENPATMANNYDWLGNLDVISFLRDIGKNFGLNYMLAKDTVASRLET
GISFTEFSYMILQSYDFLNLYQHHNCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAIW
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILELEKQVAEAPEKRAAQKALGAEMTKLVHGEEALEQAIKISA
ALFSGSVAELTASEIEQGFKDVPSVERTAEDTVLIDLLVESKISPSKRQAREDVTNGAIYVNGERTQALDYVVTEKDRIE
GKFTIIRRGKKKYFLIRY

Sequences:

>Translated_418_residues
MGILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPVLMLRRFQLAGHQPIALVGGGTGMIGDPS
GKKAERTLNTKDTVAYYTESIKNQLSNFLEFENVENPATMANNYDWLGNLDVISFLRDIGKNFGLNYMLAKDTVASRLET
GISFTEFSYMILQSYDFLNLYQHHNCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAIW
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILELEKQVAEAPEKRAAQKALGAEMTKLVHGEEALEQAIKISA
ALFSGSVAELTASEIEQGFKDVPSVERTAEDTVLIDLLVESKISPSKRQAREDVTNGAIYVNGERTQALDYVVTEKDRIE
GKFTIIRRGKKKYFLIRY
>Mature_417_residues
GILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPVLMLRRFQLAGHQPIALVGGGTGMIGDPSG
KKAERTLNTKDTVAYYTESIKNQLSNFLEFENVENPATMANNYDWLGNLDVISFLRDIGKNFGLNYMLAKDTVASRLETG
ISFTEFSYMILQSYDFLNLYQHHNCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAIWL
DPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILELEKQVAEAPEKRAAQKALGAEMTKLVHGEEALEQAIKISAA
LFSGSVAELTASEIEQGFKDVPSVERTAEDTVLIDLLVESKISPSKRQAREDVTNGAIYVNGERTQALDYVVTEKDRIEG
KFTIIRRGKKKYFLIRY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=402, Percent_Identity=36.318407960199, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI1787925, Length=420, Percent_Identity=53.0952380952381, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI25145802, Length=399, Percent_Identity=37.0927318295739, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI25145805, Length=321, Percent_Identity=34.8909657320872, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325160, Length=435, Percent_Identity=34.7126436781609, Blast_Score=281, Evalue=9e-77,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=40.9195402298851, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY1_BACAN (Q81KS9)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847109.1
- RefSeq:   YP_021554.1
- RefSeq:   YP_030803.1
- ProteinModelPortal:   Q81KS9
- EnsemblBacteria:   EBBACT00000010887
- EnsemblBacteria:   EBBACT00000016457
- EnsemblBacteria:   EBBACT00000020903
- GeneID:   1084086
- GeneID:   2818653
- GeneID:   2850360
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4911
- KEGG:   bar:GBAA_4911
- KEGG:   bat:BAS4556
- TIGR:   BA_4911
- TIGR:   GBAA_4911
- GeneTree:   EBGT00050000000599
- HOGENOM:   HBG288125
- OMA:   DRIDNQL
- ProtClustDB:   PRK05912
- BioCyc:   BANT260799:BAS4556-MONOMER
- BioCyc:   BANT261594:GBAA4911-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47036; Mature: 46905

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 34-34 BINDING 169-169 BINDING 173-173 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPVLMLRRFQL
CCCCCCCHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHQPIALVGGGTGMIGDPSGKKAERTLNTKDTVAYYTESIKNQLSNFLEFENVENPATM
CCCCCEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ANNYDWLGNLDVISFLRDIGKNFGLNYMLAKDTVASRLETGISFTEFSYMILQSYDFLNL
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
YQHHNCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAIW
HHCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCCCCCEEE
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILELEKQVAEAPEKRAAQKALGA
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHH
EMTKLVHGEEALEQAIKISAALFSGSVAELTASEIEQGFKDVPSVERTAEDTVLIDLLVE
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEHHH
SKISPSKRQAREDVTNGAIYVNGERTQALDYVVTEKDRIEGKFTIIRRGKKKYFLIRY
CCCCCHHHHHHHHHCCCEEEECCCCHHHHHEEECCCHHCCCEEEEEECCCCCEEEEEC
>Mature Secondary Structure 
GILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPVLMLRRFQL
CCCCCCHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHQPIALVGGGTGMIGDPSGKKAERTLNTKDTVAYYTESIKNQLSNFLEFENVENPATM
CCCCCEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ANNYDWLGNLDVISFLRDIGKNFGLNYMLAKDTVASRLETGISFTEFSYMILQSYDFLNL
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
YQHHNCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAIW
HHCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCCCCCEEE
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILELEKQVAEAPEKRAAQKALGA
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHH
EMTKLVHGEEALEQAIKISAALFSGSVAELTASEIEQGFKDVPSVERTAEDTVLIDLLVE
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEHHH
SKISPSKRQAREDVTNGAIYVNGERTQALDYVVTEKDRIEGKFTIIRRGKKKYFLIRY
CCCCCHHHHHHHHHCCCEEEECCCCHHHHHEEECCCHHCCCEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629