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The map label for this gene is rluF [H]

Identifier: 49187441

GI number: 49187441

Start: 4357323

End: 4358012

Strand: Reverse

Name: rluF [H]

Synonym: BAS4446

Alternate gene names: 49187441

Gene position: 4358012-4357323 (Counterclockwise)

Preceding gene: 49187442

Following gene: 49187440

Centisome position: 83.35

GC content: 37.97

Gene sequence:

>690_bases
ATGAAAATCAACCAATATATAAGCGAAGCAAATTCTTGCTCAAGACGCGAAACAGATCGCCTTATTAAAGCCGGGCGCGT
AGCGATTAACGGCGAAATATGTACGCACGGTGCACTTGTTAATGAAGGTGATGTTGTAACGATTGATGGACAGGTTATTG
CAAAAGAAGAGAAAGAAAAGGTGTATATTGCCTTTCATAAACCAGTTGGGATTACCTGCACAGCAGCTGAGCATATTGAA
GGTAATATTATTGATTATATTAATTACCCAGAGCGTATCTTTCCTGTTGGACGATTAGATAAAGCATCAGAAGGACTTAT
TTTATTAACGAATGACGGCGGGATTGCCAATCAAATTTTACACGGTGATCATGAGCACGAGAAAGAATATGTTGTTACAG
TTGATAAACCTTTTACAGATTGGTTTATTAATGAGATGTCCAGCGGTGTAAAGATTAAAGATGGAATAACTAAGCCATGT
AAAGTGACGAGAGTGGATAAGTCTACGTGTCGCATCGTTTTAACGCAAGGAATGAACAGACAAATTCGTAGAATGAGCCG
AGCTTTCGGGTTTACTGTAACAAAGTTAAAGCGAGAACGCATTATGAATATAGAGCTAAATGATCTTGAAGTAGGAAAGT
GGCGAGACATTACAGCTGAAGAATTAGAATTATTAATAGGACAGTTATAG

Upstream 100 bases:

>100_bases
TATTTATTTAGTTGTTTTTGCGAAGTAAACGAGCGTCAATCGTGGCAGAGTAATATTGCCATAGATTGACGCTTTTAATA
TATATGAAAAGGAATAAGTT

Downstream 100 bases:

>100_bases
GGAGGAAAAATTTTCTCCCTATATTTTTTTGTCAGAAAAAATAAAACTATTGATTTTTTAAAAAATAGCCCATATAATAA
AAAACAACAAGTTTCGACAA

Product: RNA pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKINQYISEANSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEKVYIAFHKPVGITCTAAEHIE
GNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQILHGDHEHEKEYVVTVDKPFTDWFINEMSSGVKIKDGITKPC
KVTRVDKSTCRIVLTQGMNRQIRRMSRAFGFTVTKLKRERIMNIELNDLEVGKWRDITAEELELLIGQL

Sequences:

>Translated_229_residues
MKINQYISEANSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEKVYIAFHKPVGITCTAAEHIE
GNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQILHGDHEHEKEYVVTVDKPFTDWFINEMSSGVKIKDGITKPC
KVTRVDKSTCRIVLTQGMNRQIRRMSRAFGFTVTKLKRERIMNIELNDLEVGKWRDITAEELELLIGQL
>Mature_229_residues
MKINQYISEANSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEKVYIAFHKPVGITCTAAEHIE
GNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQILHGDHEHEKEYVVTVDKPFTDWFINEMSSGVKIKDGITKPC
KVTRVDKSTCRIVLTQGMNRQIRRMSRAFGFTVTKLKRERIMNIELNDLEVGKWRDITAEELELLIGQL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=223, Percent_Identity=51.5695067264574, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=30.4721030042918, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=33.4763948497854, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 25855; Mature: 25855

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINQYISEANSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEK
CCCHHHHHHHHCCHHHHHHHHHHHCEEEECCEEEECCEEECCCCEEEECCEEEEECCCCE
VYIAFHKPVGITCTAAEHIEGNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQIL
EEEEEECCCCEEEEEHHCCCCCEEEECCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHH
HGDHEHEKEYVVTVDKPFTDWFINEMSSGVKIKDGITKPCKVTRVDKSTCRIVLTQGMNR
CCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEECCCCCCEEEEEECCCCEEEEEECCCCH
QIRRMSRAFGFTVTKLKRERIMNIELNDLEVGKWRDITAEELELLIGQL
HHHHHHHHHCHHHHHHHHHHEEEEEECCEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKINQYISEANSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEK
CCCHHHHHHHHCCHHHHHHHHHHHCEEEECCEEEECCEEECCCCEEEECCEEEEECCCCE
VYIAFHKPVGITCTAAEHIEGNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQIL
EEEEEECCCCEEEEEHHCCCCCEEEECCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHH
HGDHEHEKEYVVTVDKPFTDWFINEMSSGVKIKDGITKPCKVTRVDKSTCRIVLTQGMNR
CCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEECCCCCCEEEEEECCCCEEEEEECCCCH
QIRRMSRAFGFTVTKLKRERIMNIELNDLEVGKWRDITAEELELLIGQL
HHHHHHHHHCHHHHHHHHHHEEEEEECCEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]