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The map label for this gene is braB [H]

Identifier: 49187440

GI number: 49187440

Start: 4355521

End: 4356846

Strand: Reverse

Name: braB [H]

Synonym: BAS4445

Alternate gene names: 49187440

Gene position: 4356846-4355521 (Counterclockwise)

Preceding gene: 49187441

Following gene: 49187439

Centisome position: 83.33

GC content: 35.9

Gene sequence:

>1326_bases
ATGAAATCATCTTTAAAGTTTTCTGAGATGTTTGCAATAAGTTTAATGTTATTTGCACTATTTTTCGGTGCAGGTAATAT
GATTTTCCCACCAGCGTTAGGACAAGGCGCTGGAACAGATGTATGGATTGCGTTATTTGGTTTTATCGTAACAGGCGTTG
GACTACCTTTATTAGGAGTAATTGTTATAGCTTTAAAGGGAGATATTAATGAGCTAGCAGGACGTGTACATCCTATATTC
GCACTTGTTTTTATCACTGCAATTTATTTATGTTTAGGCGTATTTGTAAGTGTGCCACGTACAGGAACTGTTGCTTATGA
AATGAGTGTCGCACCGTTTTTACCAAGTGAGATAGCTGGTCAATCGTATTCACTTGTTATCTTTACATTCATTTTCTTTG
CTGTCACTTTCTATTTAGCTTTAAACCCATCGAAATTAGTGGGTCGTATTGGTAAAGTGCTAACTCCAATATTATTAGCT
GTTATTGCAATTATTGTAGGTAAAGCACTTATTACACCAATCGGAGAATTTGGTGCACCGACAGAAGCGTATAGTGCCCC
TCTTTTTAAAGGATTTATTGATGGATATTTAACTTTAGATGGACTTGCAGCACTGGTATTCGGAAATGTTGTAATTCATG
CGTTAAAAGAAAAAGGCATTACAAATAAAAACAGCATTGCAAAAGTAACAATCTTTGCAGGATTTATTGCAGCGCTTGGT
CTACTACTTGTGTATTTAGCACTTGCTTATCTTGGAGCTTCTAGTGTGTCACTTGGAATGGGCGCAAACGGAGGAATTAT
TTTAACAAATGTCGTGAATCATTTATTTGGTAGCTACGGAACTTTATTACTAGGGATCGCAATTACAGCAGCATGTTTAA
CAACTTCTGTAGGTATTGTTGCAGCTTGTGGTGATTACTTCTCTTCTTTATTACCAAAGCTTTCTTATCAAAAAGTTGTT
TTCATTTTCTGTGTATTAGCATTTATGGTAGCAAACCTTGGGTTAACTCAGTTAAATGCATTAGCATTACCAATCTTAAT
CGCAATTTATCCAATTGGAATCGTACTTATCGTATTATCACTTGTTGAAAACTACGTTCGTCTTCCATTAGCGATGTATG
TGGGCGGGATTGTAGGAGCATTTGCAATAAGTTTCTTTGATGGGTTACACAATGCAAACCTTCAAATTGCAGCGCTAGCA
CCTATTTTAGATAAGATTCCATTATATAGTGTAGGAATTGGTTGGTTAGTTCCAGGCATAATCGGTATAGGATTTGGTTA
TATAGTTTCTAAGTTTCAAAAGCAAGAAGTTGCTGTAGAAAAATAG

Upstream 100 bases:

>100_bases
GTTTTTTGCGTTCTTTTACGGGGAAGTATGCTTGAAAGAGGATATATGACGGATTTTAATTCAGAATATTTTGTTAAATT
AAATTAGGAGGTTAGTAGAA

Downstream 100 bases:

>100_bases
AGAAAAGAGGCTTTCTATAATAGAAAGTCTCTTTTTTTGTAAAAAAAGCCGTATATAGAGAAATTTAGATATTAAAAATT
TTATACGTAATATAGTTGAC

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIF
ALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQSYSLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLA
VIAIIVGKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDGLAALVFGNVVIHALKEKGITNKNSIAKVTIFAGFIAALG
LLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGTLLLGIAITAACLTTSVGIVAACGDYFSSLLPKLSYQKVV
FIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIVLSLVENYVRLPLAMYVGGIVGAFAISFFDGLHNANLQIAALA
PILDKIPLYSVGIGWLVPGIIGIGFGYIVSKFQKQEVAVEK

Sequences:

>Translated_441_residues
MKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIF
ALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQSYSLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLA
VIAIIVGKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDGLAALVFGNVVIHALKEKGITNKNSIAKVTIFAGFIAALG
LLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGTLLLGIAITAACLTTSVGIVAACGDYFSSLLPKLSYQKVV
FIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIVLSLVENYVRLPLAMYVGGIVGAFAISFFDGLHNANLQIAALA
PILDKIPLYSVGIGWLVPGIIGIGFGYIVSKFQKQEVAVEK
>Mature_441_residues
MKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIF
ALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQSYSLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLA
VIAIIVGKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDGLAALVFGNVVIHALKEKGITNKNSIAKVTIFAGFIAALG
LLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGTLLLGIAITAACLTTSVGIVAACGDYFSSLLPKLSYQKVV
FIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIVLSLVENYVRLPLAMYVGGIVGAFAISFFDGLHNANLQIAALA
PILDKIPLYSVGIGWLVPGIIGIGFGYIVSKFQKQEVAVEK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=444, Percent_Identity=35.8108108108108, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46349; Mature: 46349

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGV
CCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IVIALKGDINELAGRVHPIFALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCC
QSYSLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLAVIAIIVGKALITPIGEFGAP
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC
TEAYSAPLFKGFIDGYLTLDGLAALVFGNVVIHALKEKGITNKNSIAKVTIFAGFIAALG
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGTLLLGIAITAACLTTSVGIV
HHHHHHHHHHHCCCCCEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AACGDYFSSLLPKLSYQKVVFIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIVLS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVENYVRLPLAMYVGGIVGAFAISFFDGLHNANLQIAALAPILDKIPLYSVGIGWLVPGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHH
IGIGFGYIVSKFQKQEVAVEK
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGV
CCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IVIALKGDINELAGRVHPIFALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCC
QSYSLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLAVIAIIVGKALITPIGEFGAP
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC
TEAYSAPLFKGFIDGYLTLDGLAALVFGNVVIHALKEKGITNKNSIAKVTIFAGFIAALG
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGTLLLGIAITAACLTTSVGIV
HHHHHHHHHHHCCCCCEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AACGDYFSSLLPKLSYQKVVFIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIVLS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVENYVRLPLAMYVGGIVGAFAISFFDGLHNANLQIAALAPILDKIPLYSVGIGWLVPGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHH
IGIGFGYIVSKFQKQEVAVEK
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]