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The map label for this gene is rph

Identifier: 49187372

GI number: 49187372

Start: 4289398

End: 4290135

Strand: Reverse

Name: rph

Synonym: BAS4377

Alternate gene names: 49187372

Gene position: 4290135-4289398 (Counterclockwise)

Preceding gene: 49187373

Following gene: 49187371

Centisome position: 82.05

GC content: 40.65

Gene sequence:

>738_bases
ATGCGAGTAGATGGTAGAGAGAAAACAGAGTTACGCCATATACATATTCATACGAATTATTTAAAACATCCAGAAGGATC
AGTATTAATTGAGGTTGGGGATACAAAGGTAATTTGCTCAGCGACAATTGAAGAGCGCGTCCCGCCATTTATGCGTGGAG
AAGGAAAGGGATGGGTAACAGCTGAATATGCGATGATTCCACGTGCGACAGAACAACGTACAATTAGAGAGTCAAGTAAA
GGAAAAGTAACAGGACGTACAATGGAAATCCAGCGCTTAATTGGACGAGCATTACGTGCGGTAGTTGACTTAGAAGCGCT
TGGCGAGAGAACGGTTTGGATTGATTGTGATGTTATTCAAGCGGATGGTGGAACGCGAACGGCTTCTATTACAGGTGCAT
ATGTAGCGATGGTATTAGCTTTCGAGAAATTATTACAAGCAGAAAAAGTATCTAAAATTCCAGTAAAAGATTATTTAGCA
GCAACGTCAGTAGGAATTGTTGAAGAGCAAGGCGTTGTTTTAGATTTAAACTACGCAGAAGATTCTAAAGCAGACGTTGA
TATGAACGTGATTATGACTGGAAAAGGCCAGTTTGTTGAAGTGCAAGGAACTGGAGAAGAAGCGACGTTTAGCAGAGCAC
AGTTAAATGAATTACTTGATGCTGCGGAACAAGGCATTTTCCAACTGATTGATATTCAAAAAGAAGCGTTAGGTGACATC
GTATCTCATATAGAGTAG

Upstream 100 bases:

>100_bases
GAAAGAGGAATTGCTTTTTGGGTTCCTCTTCTTTTATTGTATACATATCATACATTTAAATTTGGCTATACTAACAAGTA
GTTATGAGGAGGTTATTTTT

Downstream 100 bases:

>100_bases
AGGTGGAAAATATGAAACAAGTTGTTGTAGCGACAAAAAATATGGGGAAAGTACGTGAATTTGCTGAGTTATTTGAGCGA
TTTGATTTAGAAGTAAAATC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRVDGREKTELRHIHIHTNYLKHPEGSVLIEVGDTKVICSATIEERVPPFMRGEGKGWVTAEYAMIPRATEQRTIRESSK
GKVTGRTMEIQRLIGRALRAVVDLEALGERTVWIDCDVIQADGGTRTASITGAYVAMVLAFEKLLQAEKVSKIPVKDYLA
ATSVGIVEEQGVVLDLNYAEDSKADVDMNVIMTGKGQFVEVQGTGEEATFSRAQLNELLDAAEQGIFQLIDIQKEALGDI
VSHIE

Sequences:

>Translated_245_residues
MRVDGREKTELRHIHIHTNYLKHPEGSVLIEVGDTKVICSATIEERVPPFMRGEGKGWVTAEYAMIPRATEQRTIRESSK
GKVTGRTMEIQRLIGRALRAVVDLEALGERTVWIDCDVIQADGGTRTASITGAYVAMVLAFEKLLQAEKVSKIPVKDYLA
ATSVGIVEEQGVVLDLNYAEDSKADVDMNVIMTGKGQFVEVQGTGEEATFSRAQLNELLDAAEQGIFQLIDIQKEALGDI
VSHIE
>Mature_245_residues
MRVDGREKTELRHIHIHTNYLKHPEGSVLIEVGDTKVICSATIEERVPPFMRGEGKGWVTAEYAMIPRATEQRTIRESSK
GKVTGRTMEIQRLIGRALRAVVDLEALGERTVWIDCDVIQADGGTRTASITGAYVAMVLAFEKLLQAEKVSKIPVKDYLA
ATSVGIVEEQGVVLDLNYAEDSKADVDMNVIMTGKGQFVEVQGTGEEATFSRAQLNELLDAAEQGIFQLIDIQKEALGDI
VSHIE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=59.2417061611374, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71981632, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_BACAA (C3PA74)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868764.1
- ProteinModelPortal:   C3PA74
- SMR:   C3PA74
- EnsemblBacteria:   EBBACT00000124675
- GeneID:   7852274
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4732
- GeneTree:   EBGT00050000001825
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26983; Mature: 26983

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDGREKTELRHIHIHTNYLKHPEGSVLIEVGDTKVICSATIEERVPPFMRGEGKGWVT
CCCCCCCCCEEEEEEEEECEEECCCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCCEEE
AEYAMIPRATEQRTIRESSKGKVTGRTMEIQRLIGRALRAVVDLEALGERTVWIDCDVIQ
EEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAYVAMVLAFEKLLQAEKVSKIPVKDYLAATSVGIVEEQGVVLDLNYAE
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCEEEEEECCC
DSKADVDMNVIMTGKGQFVEVQGTGEEATFSRAQLNELLDAAEQGIFQLIDIQKEALGDI
CCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSHIE
HHHCC
>Mature Secondary Structure
MRVDGREKTELRHIHIHTNYLKHPEGSVLIEVGDTKVICSATIEERVPPFMRGEGKGWVT
CCCCCCCCCEEEEEEEEECEEECCCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCCEEE
AEYAMIPRATEQRTIRESSKGKVTGRTMEIQRLIGRALRAVVDLEALGERTVWIDCDVIQ
EEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAYVAMVLAFEKLLQAEKVSKIPVKDYLAATSVGIVEEQGVVLDLNYAE
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCEEEEEECCC
DSKADVDMNVIMTGKGQFVEVQGTGEEATFSRAQLNELLDAAEQGIFQLIDIQKEALGDI
CCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSHIE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA