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The map label for this gene is hemC

Identifier: 49187356

GI number: 49187356

Start: 4272282

End: 4273211

Strand: Reverse

Name: hemC

Synonym: BAS4361

Alternate gene names: 49187356

Gene position: 4273211-4272282 (Counterclockwise)

Preceding gene: 49187357

Following gene: 49187355

Centisome position: 81.73

GC content: 39.68

Gene sequence:

>930_bases
ATGCGCAAAATTATTGTAGGGTCACGAAAGAGTAAATTAGCATTAACGCAAACAAACTGGTTTATTGATCAGTTAAAAGC
GCTAGGTTTACCATATGAATTTGAAGTGAAAGAAATCGTCACAAAAGGTGATGTTATTTTAGATGTTACTCTTTCAAAAG
TAGGCGGAAAAGGTTTGTTTGTGAAAGAGATTGAACATGCGTTACTTACGAAAGAAATTGATATGGCTGTACATAGTATG
AAAGATATGCCGGCCGTACTTCCAGAAGGATTAATGATCGGTTGTACACCAAAGCGTGTTGACCCTCGTGATGCTTTTAT
CTCAAAAAGTGGAGCGTCGTATAAAGAGTTAGCAGAAGGCGCTATTTTAGGTACGAGTAGTTTAAGACGTAGTGCACAGC
TGCTCGCTGCAAGACCAGATTTACAAGTGAAGTGGATTCGCGGAAATATCGATACACGTTTACGCAAATTAAAAGAAGAA
GATTACGATGCAATTATTTTAGCGACGGCGGGATTACAAAGAATGGGCTGGGATAATGAAGTTATTACAGAACATTTAGA
TGAGACGTTATGTGTACCTGCTGTAGGACAAGGCGCATTAGCGATTGAGTGTCGTGAGGATGATAAGGATTTACTACAGT
TGTTAGCGCATATAAATGATGCAGTAACAGAGAAAACAGTGGCAGCAGAACGAGTATTTCTTCATAAACTTGAAGGTGGA
TGCCAAGTTCCGATCGCTGGATATGCAACTATTACAGAAAACGATGCAATCGAATTAACAGCTCTTGTCGGGTCTATGGA
TGGTTCTGTTTTATTGAAAGAGACAGTAGTAGGAACTGATCCAGAGAAAGTAGGATTAGAGGCTGCGGACCGTTTAATTA
AGCAAGGCGCAAAAGAACTCATTCTTGCTGCAAATAAGGGGCAACAATAA

Upstream 100 bases:

>100_bases
GTGGAACATTGGGGCATTTCTCGTAATGCTAATTAATATTTTTCTATTAAGCAGTTTATCCAACTTCCACTTTTGGTATT
TATAATGAGGAGAGAAAATT

Downstream 100 bases:

>100_bases
TATGAACGCTCTCGCTGGCAAAACGGTATTGATTACACGTGCTCAGCACCAAGCAAAACAAATGAGTGTAGCGGTGAAAG
AAAAGAGCGGAATTCCATTG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLFVKEIEHALLTKEIDMAVHSM
KDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASYKELAEGAILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEE
DYDAIILATAGLQRMGWDNEVITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTEKTVAAERVFLHKLEGG
CQVPIAGYATITENDAIELTALVGSMDGSVLLKETVVGTDPEKVGLEAADRLIKQGAKELILAANKGQQ

Sequences:

>Translated_309_residues
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLFVKEIEHALLTKEIDMAVHSM
KDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASYKELAEGAILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEE
DYDAIILATAGLQRMGWDNEVITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTEKTVAAERVFLHKLEGG
CQVPIAGYATITENDAIELTALVGSMDGSVLLKETVVGTDPEKVGLEAADRLIKQGAKELILAANKGQQ
>Mature_309_residues
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLFVKEIEHALLTKEIDMAVHSM
KDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASYKELAEGAILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEE
DYDAIILATAGLQRMGWDNEVITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTEKTVAAERVFLHKLEGG
CQVPIAGYATITENDAIELTALVGSMDGSVLLKETVVGTDPEKVGLEAADRLIKQGAKELILAANKGQQ

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=315, Percent_Identity=46.3492063492063, Blast_Score=244, Evalue=5e-65,
Organism=Homo sapiens, GI20149500, Length=315, Percent_Identity=46.3492063492063, Blast_Score=244, Evalue=6e-65,
Organism=Escherichia coli, GI48994974, Length=304, Percent_Identity=47.6973684210526, Blast_Score=256, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=38.9240506329114, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI20130425, Length=309, Percent_Identity=41.1003236245955, Blast_Score=204, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_BACAA (C3P9E9)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868748.1
- ProteinModelPortal:   C3P9E9
- SMR:   C3P9E9
- EnsemblBacteria:   EBBACT00000124930
- GeneID:   7852500
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4714
- GeneTree:   EBGT00050000001150
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33663; Mature: 33663

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLF
CCCEEECCCCCEEEEEEHHHHHHHHHHHCCCCEEHHHHHHCCCCEEEEEEEHHCCCCCEE
VKEIEHALLTKEIDMAVHSMKDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASYKELAEG
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHCC
AILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEEDYDAIILATAGLQRMGWDNE
CEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEECCHHHCCCCHH
VITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTEKTVAAERVFLHKLEGG
HHHHHHCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CQVPIAGYATITENDAIELTALVGSMDGSVLLKETVVGTDPEKVGLEAADRLIKQGAKEL
CCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEHHCCCCHHHHCHHHHHHHHHHCHHHE
ILAANKGQQ
EEECCCCCC
>Mature Secondary Structure
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLF
CCCEEECCCCCEEEEEEHHHHHHHHHHHCCCCEEHHHHHHCCCCEEEEEEEHHCCCCCEE
VKEIEHALLTKEIDMAVHSMKDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASYKELAEG
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHCC
AILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEEDYDAIILATAGLQRMGWDNE
CEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEECCHHHCCCCHH
VITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTEKTVAAERVFLHKLEGG
HHHHHHCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CQVPIAGYATITENDAIELTALVGSMDGSVLLKETVVGTDPEKVGLEAADRLIKQGAKEL
CCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEHHCCCCHHHHCHHHHHHHHHHCHHHE
ILAANKGQQ
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA