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The map label for this gene is sigK

Identifier: 49187232

GI number: 49187232

Start: 4150337

End: 4151050

Strand: Reverse

Name: sigK

Synonym: BAS4236

Alternate gene names: 49187232

Gene position: 4151050-4150337 (Counterclockwise)

Preceding gene: 49187235

Following gene: 49187231

Centisome position: 79.39

GC content: 36.97

Gene sequence:

>714_bases
TTGAGTCTATTCGCCGCAATTGGATATATGGTTCGAGAAGTGTTTGTTTTTGTTTCTTATGTGAAGAACAATGCGTTTCC
GCAGCCATTATCATCAGACGATGAGAGAAAGTACTTAGAGTTAATGGAGCAAGGTGATGCTCAAGCGAGGAATCTGTTAA
TTGAACATAATTTACGGCTTGTAGCTCATATCGTTAAAAAATTTGAAAATACAGGGGAAGATGCAGAAGATTTAATTTCA
ATTGGTACAATCGGGCTCATTAAAGCAATCGAGAGCTATTCGGCAGGAAAAGGTACAAAACTTGCGACGTACGCAGCACG
CTGTATTGAAAATGAAATTTTGATGCATTTACGTGTATTAAAGAAAACGAAAAAGGACGTTTCACTTCATGATCCAATCG
GGCAAGATAAAGAGGGGAATGAAATATCGCTTATTGATATATTAAAATCAGAGTCTGAAGATGTAATTGACATGATCCAG
CTTAGTATGGAGTTAGAAAAGATTAAAGAGTATATCGATATTTTAGACGAACGAGAGAAAGAAGTAATCGTGAAGCGTTT
TGGACTGGGGCTTGATAAGGAGAAGACGCAACGAGAGATTGCGAAGGCACTTGGTATTTCCAGAAGCTATGTATCAAGAA
TTGAAAAGCGCGCTTTAATGAAAATGTTCCATGAATTTGTAAGAGCAGAGAAAGAGAAAAAAGCAAAAGAATAA

Upstream 100 bases:

>100_bases
ATTGGAAGTGTTGAGTTTTATAAATAAACTGTACAGACACAAACAACCTCACGCCCACATACACTTAGTAAAGACCACTA
TTTTGGCGGAGGTGAAAGGT

Downstream 100 bases:

>100_bases
TGTACATTACAAGCAGCCGTATTTTAAAATGGGCTGCTTTTCTTTTTGGAAATAAGGAAAGAAAAAGAGTTGTGTTCAGA
AGAAATCCATGTTCACTGTT

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQ
LSMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEKKAKE

Sequences:

>Translated_237_residues
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQ
LSMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEKKAKE
>Mature_236_residues
SLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLISI
GTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQL
SMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEKKAKE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=24.21875, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789871, Length=109, Percent_Identity=37.6146788990826, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): RP28_BACAN (P62182)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846788.1
- RefSeq:   YP_021211.1
- RefSeq:   YP_030484.1
- ProteinModelPortal:   P62182
- SMR:   P62182
- EnsemblBacteria:   EBBACT00000010179
- EnsemblBacteria:   EBBACT00000016313
- EnsemblBacteria:   EBBACT00000019367
- GeneID:   1088335
- GeneID:   2818972
- GeneID:   2852916
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4566
- KEGG:   bar:GBAA_4566
- KEGG:   bat:BAS4236
- TIGR:   BA_4566
- TIGR:   GBAA_4566
- GeneTree:   EBGT00070000031774
- HOGENOM:   HBG745096
- OMA:   IVEARIM
- ProtClustDB:   PRK05803
- BioCyc:   BANT260799:BAS4236-MONOMER
- BioCyc:   BANT261594:GBAA4566-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF000770
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02846

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 27137; Mature: 27006

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQLSMELEKIKEYIDILDEREK
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEKKAKE
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQLSMELEKIKEYIDILDEREK
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEKKAKE
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629